Literature DB >> 368649

Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm.

P Bassford, J Beckwith.   

Abstract

The maltose binding protein of Escherichia coli is secreted into the external periplasmic compartment of the cell. This article describes a selection procedure for the isolation of mutants which fail to export this protein. These mutants probably result from alterations in the amino terminal 'signal sequence', causing the maltose binding protein produced to accumulate in the cytoplasm in its precursor form.

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Year:  1979        PMID： 368649     DOI： 10.1038/277538a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  56 in total

1.  Green fluorescent protein functions as a reporter for protein localization in Escherichia coli.

Authors:  B J Feilmeier; G Iseminger; D Schroeder; H Webber; G J Phillips
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

2.  Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm.

Authors:  P J Bassford; T J Silhavy; J R Beckwith
Journal:  J Bacteriol       Date:  1979-07       Impact factor: 3.490

Review 3.  The sec and prl genes of Escherichia coli.

Authors:  K L Bieker; G J Phillips; T J Silhavy
Journal:  J Bioenerg Biomembr       Date:  1990-06       Impact factor: 2.945

4.  Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

Authors:  J P Fandl; P C Tai
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

Review 5.  The chemistry and enzymology of the type I signal peptidases.

Authors:  R E Dalbey; M O Lively; S Bron; J M van Dijl
Journal:  Protein Sci       Date:  1997-06       Impact factor: 6.725

6.  A mutation in the amino terminus of a hybrid TrpC-TonB protein relieves overproduction lethality and results in cytoplasmic accumulation.

Authors:  J T Skare; S K Roof; K Postle
Journal:  J Bacteriol       Date:  1989-08       Impact factor: 3.490

7.  Role of the leader peptide of maltose-binding protein in two steps of the export process.

Authors:  J R Thom; L L Randall
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

8.  An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins.

Authors:  K L Strauch; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

Review 9.  The Sec-dependent pathway.

Authors:  Jon Beckwith
Journal:  Res Microbiol       Date:  2013-03-26       Impact factor: 3.992

10.  In vitro synthesis of a putative precursor of mitochondrial ornithine transcarbamoylase.

Authors:  J G Conboy; F Kalousek; L E Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205
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