Literature DB >> 3682063

Effect of NH4+ ions on phi 29 DNA-protein p3 replication: formation of a complex between the terminal protein and the DNA polymerase.

L Blanco1, I Prieto, J Gutiérrez, A Bernad, J M Lázaro, J M Hermoso, M Salas.   

Abstract

Ammonium ions stimulated the formation of the phi diameter 29 protein p3-dAMP initiation complex by decreasing the Km value for dATP in a purified system containing the viral terminal protein p3, the viral DNA polymerase p2, and the phi 29 DNA-protein p3 complex as a template. In addition, NH4+ ions stimulated the amount of p3-dAMP complex elongation and increased by about twofold the rate of elongation. The stimulatory effect of NH4+ ions on in vitro phi 29 DNA replication is probably related to the formation of a stable complex between the terminal protein and the DNA polymerase, which was detected only in the presence of NH4+ ions.

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Year:  1987        PMID： 3682063      PMCID： PMC256019     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  30 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Cloning and template activity of the origins of replication of phage phi 29 DNA.

Authors:  J Gutiérrez; J A García; L Blanco; M Salas
Journal:  Gene       Date:  1986       Impact factor: 3.688

3.  Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors:  M W McDonell; M N Simon; F W Studier
Journal:  J Mol Biol       Date:  1977-02-15       Impact factor: 5.469

4.  Nucleotide sequences at the termini of phi 29 DNA.

Authors:  H Yoshikawa; T Friedmann; J Ito
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205

5.  The protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi 29 is involved in the initiation of DNA replication.

Authors:  R P Mellado; M A Peñalva; M R Inciarte; M Salas
Journal:  Virology       Date:  1980-07-15       Impact factor: 3.616

6.  Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29.

Authors:  M R Inciarte; M Salas; J M Sogo
Journal:  J Virol       Date:  1980-04       Impact factor: 5.103

7.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

8.  DNA replication of bacteriophage phi 29: characterization of the intermediates and location of the termini of replication.

Authors:  N E Harding; J Ito
Journal:  Virology       Date:  1980-07-30       Impact factor: 3.616

9.  DNA-protein complex in circular DNA from phage phi-29.

Authors:  J Ortĭn; E Viñuela; M Salas; C Vasquez
Journal:  Nat New Biol       Date:  1971-12-29

10.  Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.

Authors:  C Escarmís; M Salas
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205
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  22 in total

1.  Phi29 family of phages.

Authors:  W J Meijer; J A Horcajadas; M Salas
Journal:  Microbiol Mol Biol Rev       Date:  2001-06       Impact factor: 11.056

2.  The integral membrane protein p16.7 organizes in vivo phi29 DNA replication through interaction with both the terminal protein and ssDNA.

Authors:  Alejandro Serna-Rico; Daniel Muñoz-Espín; Laurentino Villar; Margarita Salas; Wilfried J J Meijer
Journal:  EMBO J       Date:  2003-05-01       Impact factor: 11.598

3.  Initiation of phi 29 DNA replication occurs at the second 3' nucleotide of the linear template: a sliding-back mechanism for protein-primed DNA replication.

Authors:  J Méndez; L Blanco; J A Esteban; A Bernad; M Salas
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

4.  Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage Phi29.

Authors:  Mario Mencía; Pablo Gella; Ana Camacho; Miguel de Vega; Margarita Salas
Journal:  Proc Natl Acad Sci U S A       Date:  2011-11-07       Impact factor: 11.205

5.  Viral terminal protein directs early organization of phage DNA replication at the bacterial nucleoid.

Authors:  Daniel Muñoz-Espín; Isabel Holguera; David Ballesteros-Plaza; Rut Carballido-López; Margarita Salas
Journal:  Proc Natl Acad Sci U S A       Date:  2010-09-07       Impact factor: 11.205

6.  Structural and functional analysis of temperature-sensitive mutants of the phage phi 29 DNA polymerase.

Authors:  M A Blasco; L Blanco; E Parés; M Salas; A Bernad
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

7.  Insights into the Determination of the Templating Nucleotide at the Initiation of φ29 DNA Replication.

Authors:  Alicia Del Prado; José M Lázaro; Elisa Longás; Laurentino Villar; Miguel de Vega; Margarita Salas
Journal:  J Biol Chem       Date:  2015-09-23       Impact factor: 5.157

8.  The phi29 DNA polymerase:protein-primer structure suggests a model for the initiation to elongation transition.

Authors:  Satwik Kamtekar; Andrea J Berman; Jimin Wang; José M Lázaro; Miguel de Vega; Luis Blanco; Margarita Salas; Thomas A Steitz
Journal:  EMBO J       Date:  2006-03-02       Impact factor: 11.598

9.  Phage phi29 and Nf terminal protein-priming domain specifies the internal template nucleotide to initiate DNA replication.

Authors:  Elisa Longás; Laurentino Villar; José M Lázaro; Miguel de Vega; Margarita Salas
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-14       Impact factor: 11.205

10.  Protein-primed DNA replication: a transition between two modes of priming by a unique DNA polymerase.

Authors:  J Mendez; L Blanco; M Salas
Journal:  EMBO J       Date:  1997-05-01       Impact factor: 11.598
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