Literature DB >> 3682001

Crystallization and a preliminary X-ray diffraction study of isozyme 3-3 of glutathione S-transferase from rat liver.

M A Sesay1, H L Ammon, R N Armstrong.   

Abstract

Crystals of the homodimeric isozyme 3-3 of glutathione S-transferase from rat liver have been obtained with the hanging drop method of vapor diffusion from ammonium sulfate solutions. The successful crystallization of the enzyme required the presence of both the enzyme inhibitor (9R, 10R)-9, 10-dihydro-9-(S-glutathionyl)-10-hydroxyphenanthrene and the detergent beta-octylglucopyranoside. The crystals belong to the monoclinic space group C2, with cell dimensions of a = 88.24(8) A, b = 69.44(4) A, c = 81.28(5) A, beta = 106.01(6) degrees, and contain four dimeric enzyme molecules per unit cell. The crystals diffract to at least 2.2 A and are suitable for X-ray crystallographic structure determination at high resolution.

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Year:  1987        PMID： 3682001     DOI： 10.1016/0022-2836(87)90133-1

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  6 in total

1.  Substrate specificity of rat liver glutathione S-transferase isoenzymes for a series of glutathione analogues, modified at the gamma-glutamyl moiety.

Authors:  A E Adang; J Brussee; D J Meyer; B Coles; B Ketterer; A van der Gen; G J Mulder
Journal:  Biochem J       Date:  1988-10-15       Impact factor: 3.857

2.  The contribution of the C-terminal sequence to the catalytic activity of GST2, a human alpha-class glutathione transferase.

Authors:  P G Board; B Mannervik
Journal:  Biochem J       Date:  1991-04-01       Impact factor: 3.857

3.  Effects of directed mutagenesis on conserved arginine residues in a human Class Alpha glutathione transferase.

Authors:  G Stenberg; P G Board; I Carlberg; B Mannervik
Journal:  Biochem J       Date:  1991-03-01       Impact factor: 3.857

4.  Site-directed mutagenesis and chemical modification of cysteine residues of rat glutathione S-transferase 3-3.

Authors:  W L Chen; J C Hsieh; J L Hong; S P Tsai; M F Tam
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

5.  Inhibition of glutathione S-transferase 3-3 by glutathione derivatives that bind covalently to the active site.

Authors:  A E Adang; W J Moree; J Brussee; G J Mulder; A van der Gen
Journal:  Biochem J       Date:  1991-08-15       Impact factor: 3.857

6.  The three-dimensional structure of class pi glutathione S-transferase in complex with glutathione sulfonate at 2.3 A resolution.

Authors:  P Reinemer; H W Dirr; R Ladenstein; J Schäffer; O Gallay; R Huber
Journal:  EMBO J       Date:  1991-08       Impact factor: 11.598
  6 in total

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