| Literature DB >> 368065 |
N J Oppenheimer, M Singh, C C Sweeley, S J Sung, P A Srere.
Abstract
The structure of the prosthetic group of citrate lyase (Klebsiella aerogenes) was studied by nuclear magnetic resonance and mass spectrometry. The spectra at 360 MHz of the nucleoside moiety (2'-ribosyladenosine) show the absence of 2'-hydroxyl proton, thus confirming the 2' position as the site of attachment of the second ribose moiety to the dephospho-CoA. This glycosidic linkage is found to be alpha(1" leads to 2') and is identical to that of poly(ADP-ribose). Studies of permethylation products by mass spectrometry support the above conclusion regarding the location of the ribosidic linkage.Entities:
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Year: 1979 PMID: 368065
Source DB: PubMed Journal: J Biol Chem ISSN: 0021-9258 Impact factor: 5.157