| Literature DB >> 3680207 |
J Hesse1, M Thierauf, H Ponstingl.
Abstract
Assembly-competent microtubule protein was directly photoaffinity labeled with [alpha-32P]guanosine triphosphate by UV irradiation. The labeled tubulin was digested with trypsin. The radioactive fragments were isolated and sequenced, revealing beta-tubulin residues 155-174 to be the major labeled region. An antibody to a synthetic peptide comprising residues beta 154-165 inhibits GTP incorporation and tubulin polymerization.Entities:
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Year: 1987 PMID: 3680207
Source DB: PubMed Journal: J Biol Chem ISSN: 0021-9258 Impact factor: 5.157