Literature DB >> 3679699

A new HPLC-method for the simultaneous determination of B1-, B2- and B6-vitamers in serum and whole blood.

B Bötticher1, D Bötticher.   

Abstract

Considering that the B1-, B2- and B6-vitamers in whole blood and serum are partly protein bound and partly in phosphorylated form as co-enzyme, we developed a sample treatment which is able to transduce all three vitamers in their free forms. After cleaning the sample on solid-phase-extraction columns (SPE-column) we are able to separate all three vitamers in one run with HPLC and to quantify them with simultaneous detection (UV and Fluorescence). The following reference values were obtained: (ng/ml) Serum: Thiamin 6.55 +/- 2.38, Riboflavin 4.92 +/- 1.01, vitamin B6 11.21 +/- 3.17. Whole blood: Thiamin 62.3 +/- 20.8, riboflavin 69.8 +/- 20.9, vitamin B6 59.5 +/- 22.4.

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Year:  1987        PMID： 3679699

Source DB:  PubMed          Journal:  Int J Vitam Nutr Res        ISSN： 0300-9831            Impact factor:   1.784


  5 in total

1.  [Vitamin B1, B2, A and E and beta-carotene content in transitional breast milk and comparative studies in maternal and umbilical cord blood].

Authors:  V Böhm; G Peiker; A Starker; E Weske; G Schaarmann; R Schubert; R Bitsch; G Flachowsky
Journal:  Z Ernahrungswiss       Date:  1997-09

2.  Thiamin transport by human erythrocytes and ghosts.

Authors:  D Casirola; C Patrini; G Ferrari; G Rindi
Journal:  J Membr Biol       Date:  1990-10       Impact factor: 1.843

3.  Thiamine transport by erythrocytes and ghosts in thiamine-responsive megaloblastic anaemia.

Authors:  G Rindi; D Casirola; V Poggi; B De Vizia; C Patrini; U Laforenza
Journal:  J Inherit Metab Dis       Date:  1992       Impact factor: 4.982

4.  Status of B-vitamins and homocysteine in diabetic retinopathy: association with vitamin-B12 deficiency and hyperhomocysteinemia.

Authors:  Alleboena Satyanarayana; Nagalla Balakrishna; Sujatha Pitla; Paduru Yadagiri Reddy; Sivaprasad Mudili; Pratti Lopamudra; Palla Suryanarayana; Kalluru Viswanath; Radha Ayyagari; Geereddy Bhanuprakash Reddy
Journal:  PLoS One       Date:  2011-11-01       Impact factor: 3.240

5.  Data representing two separate LC-MS methods for detection and quantification of water-soluble and fat-soluble vitamins in tears and blood serum.

Authors:  Maryam Khaksari; Lynn R Mazzoleni; Chunhai Ruan; Robert T Kennedy; Adrienne R Minerick
Journal:  Data Brief       Date:  2017-02-16
  5 in total

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