Literature DB >> 3677069

Characterization of a novel reaction by human O6-alkylguanine-DNA alkyltransferase with 1,3-bis(2-chloroethyl)-1-nitrosourea-treated DNA.

T P Brent1, J S Remack, D G Smith.   

Abstract

The reaction of partially purified human O6-alkylguanine-DNA alkyltransferase with 1,3-bis(2-chloroethyl)-1-nitrosourea-treated DNA produces a covalent DNA-protein complex. Evidence that the complex-forming and the alkyltransferase activities are one and the same includes their copurification, identical heat inactivation kinetics, and similar responses to inhibitors. This novel reaction thus provides a sensitive alternative assay for detecting the alkyltransferase. It also provides support for a model in which N1,O6-ethanoguanine is an intermediate in formation of chloroethylnitrosourea-induced DNA interstrand cross-links.

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Year:  1987        PMID： 3677069

Source DB:  PubMed          Journal:  Cancer Res        ISSN： 0008-5472            Impact factor:   12.701


  4 in total

1.  Evidence that covalent complex formation between BCNU-treated oligonucleotides and E. coli alkyltransferases requires the O6-alkylguanine function.

Authors:  P E Gonzaga; L Harris; G P Margison; T P Brent
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

2.  Covalent capture of a human O(6)-alkylguanine alkyltransferase-DNA complex using N(1),O(6)-ethanoxanthosine, a mechanism-based crosslinker.

Authors:  D M Noll; N D Clarke
Journal:  Nucleic Acids Res       Date:  2001-10-01       Impact factor: 16.971

3.  Chemosensitivity testing of human gliomas using a fluorescent microcarrier technique.

Authors:  A P Bowles; C G Pantazis; W Wansley; M B Allen
Journal:  J Neurooncol       Date:  1990-04       Impact factor: 4.130

4.  Formation of covalent complexes between human O6-alkylguanine-DNA alkyltransferase and BCNU-treated defined length synthetic oligodeoxynucleotides.

Authors:  T P Brent; J S Remack
Journal:  Nucleic Acids Res       Date:  1988-07-25       Impact factor: 16.971
  4 in total

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