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Literature DB >> 3674411

A method for the production of highly specific polyclonal antibodies.

Abstract

Two polyclonal antibodies directed against paramyosin and tropomyosin from Owenia fusiformis (a marine polychete annelid) were obtained using a new method of immunization. After purification by two-dimensional gel electrophoresis, proteins were transferred onto a nitrocellulose sheet using the Western blot technique. The proteins bound to their cellulose support were injected into rabbits without Freund's adjuvant and without solubilization of nitrocellulose with dimethyl sulfoxide. Highly specific polyclonal antibodies were generated.

Entities: Chemical Species

Mesh：

Substances：
Tropomyosin

Year: 1987 PMID： 3674411 DOI： 10.1016/0003-2697(87)90568-9

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

12 in total

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2. Lectin localization in human nerve by biochemically defined lectin-binding glycoproteins, neoglycoprotein and lectin-specific antibody.

Authors: H J Gabius; B Wosgien; M Hendrys; A Bardosi
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4. Cloning and expression in Escherichia coli of the dnaK gene of Zymomonas mobilis.

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Journal: J Bacteriol Date: 1993-05 Impact factor: 3.490

5. Cloning, characterization, and functional studies of a human 40-kDa catecholamine-regulated protein: implications in central nervous system disorders.

Authors: Joseph Gabriele; Giuseppe F Pontoriero; Nancy Thomas; Christy A Thomson; Kevin Skoblenick; Zdenek B Pristupa; Ram K Mishra
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6. Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus.

Authors: M A Stein; S A McAllister; B E Torian; D L Diedrich
Journal: J Bacteriol Date: 1992-05 Impact factor: 3.490

A method for the production of highly specific polyclonal antibodies.

1. Immunological crossreactivity between the retinal Na(+)-Ca2+,K+ and the cardiac Na(+)-Ca2+ exchanger proteins.

2. Lectin localization in human nerve by biochemically defined lectin-binding glycoproteins, neoglycoprotein and lectin-specific antibody.

3. Amalgam is a ligand for the transmembrane receptor neurotactin and is required for neurotactin-mediated cell adhesion and axon fasciculation in Drosophila.

4. Cloning and expression in Escherichia coli of the dnaK gene of Zymomonas mobilis.

5. Cloning, characterization, and functional studies of a human 40-kDa catecholamine-regulated protein: implications in central nervous system disorders.

6. Acquisition of apparently intact and unmodified lipopolysaccharides from Escherichia coli by Bdellovibrio bacteriovorus.

7. GEBF-I in Drosophila species and hybrids: the co-evolution of an enhancer and its cognate factor.

8. Scanning independent ribosomal initiation of the Sendai virus Y proteins in vitro and in vivo.

9. Fish zona radiata (eggshell) protein: a sensitive biomarker for environmental estrogens.

10. Vectorial targeting of an endogenous apical membrane sialoglycoprotein and uvomorulin in MDCK cells.