Literature DB >> 3674409

A colorimetric assay for alpha-hydroxynitrile lyase.

D Selmar1, F J Carvalho, E E Conn.   

Abstract

A colorimetric assay for alpha-hydroxynitrile lyase which utilizes acetone cyanohydrin as a substrate is described. The assay is based on measurement of the HCN formed when the lyase catalyzes the dissociation of acetone cyanohydrin. The procedure was devised for use with the optically inactive acetone cyanohydrin but will be applicable to enzymes utilizing other cyanohydrins.

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Year:  1987        PMID： 3674409     DOI： 10.1016/0003-2697(87)90565-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Evolution of a Catalytic Mechanism.

Authors:  Alissa Rauwerdink; Mark Lunzer; Titu Devamani; Bryan Jones; Joanna Mooney; Zhi-Jun Zhang; Jian-He Xu; Romas J Kazlauskas; Antony M Dean
Journal:  Mol Biol Evol       Date:  2015-12-16       Impact factor: 16.240

2.  Mandelonitrile lyase from Ximenia americana L.: stereospecificity and lack of flavin prosthetic group.

Authors:  G W Kuroki; E E Conn
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

3.  Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta.

Authors:  Hanspeter Lauble; Burkhard Miehlich; Siegfried Förster; Christoph Kobler; Harald Wajant; Franz Effenberger
Journal:  Protein Sci       Date:  2002-01       Impact factor: 6.725

4.  Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from Manihot esculenta in complex with acetone cyanohydrin.

Authors:  H Lauble; B Miehlich; S Förster; H Wajant; F Effenberger
Journal:  Protein Sci       Date:  2001-05       Impact factor: 6.725

5.  Burning vegetation produces cyanohydrins that liberate cyanide and stimulate seed germination.

Authors:  Gavin R Flematti; David J Merritt; Matthew J Piggott; Robert D Trengove; Steven M Smith; Kingsley W Dixon; Emilio L Ghisalberti
Journal:  Nat Commun       Date:  2011-06-21       Impact factor: 14.919

6.  Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism.

Authors:  Bryan J Jones; Zsófia Bata; Romas J Kazlauskas
Journal:  ACS Catal       Date:  2017-05-15       Impact factor: 13.084

7.  Catalytic Promiscuity of Ancestral Esterases and Hydroxynitrile Lyases.

Authors:  Titu Devamani; Alissa M Rauwerdink; Mark Lunzer; Bryan J Jones; Joanna L Mooney; Maxilmilien Alaric O Tan; Zhi-Jun Zhang; Jian-He Xu; Antony M Dean; Romas J Kazlauskas
Journal:  J Am Chem Soc       Date:  2016-01-15       Impact factor: 15.419

8.  The cyanogenic syndrome in rubber tree Hevea brasiliensis: tissue-damage-dependent activation of linamarase and hydroxynitrile lyase accelerates hydrogen cyanide release.

Authors:  Daniel Kadow; Karsten Voß; Dirk Selmar; Reinhard Lieberei
Journal:  Ann Bot       Date:  2012-03-25       Impact factor: 4.357
  8 in total

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