Literature DB >> 3674377

Elimination of CoASH interference from acetyl-CoA cycling assay by maleic anhydride.

A Szutowicz1, H Bielarczyk.   

Abstract

A method for the removal of CoASH from tissue extracts by maleic anhydride is described. It eliminates CoASH interference in the acetyl-CoA cycling assay using phosphotransacetylase and citrate synthase. Maleyl-CoA thioether does not hydrolyze under the conditions of the assay and allows a reduction in the number of blank samples during acetyl-CoA determination. The levels of acetyl-CoA in whole rat brain, isolated synaptosomes, and mitochondria were found to be 61, 8.6, and 31.3 pmol/mg of protein, respectively.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3674377     DOI： 10.1016/0003-2697(87)90495-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

1.  Phenotype-dependent susceptibility of cholinergic neuroblastoma cells to neurotoxic inputs.

Authors:  A Szutowicz; H Bielarczyk; S Gul; A Ronowska; T Pawełczyk; A Jankowska-Kulawy
Journal:  Metab Brain Dis       Date:  2006-05-25       Impact factor: 3.584

2.  Analysis of Coenzymes and Antioxidants in Tissue and Blood Using 1D 1H NMR Spectroscopy.

Authors:  G A Nagana Gowda; Daniel Raftery
Journal:  Methods Mol Biol       Date:  2019

3.  Acetate-dependent mechanisms of inborn tolerance to ethanol.

Authors:  Sergey M Zimatkin; Nikolay A Oganesian; Yury V Kiselevski; Richard A Deitrich
Journal:  Alcohol Alcohol       Date:  2011-02-24       Impact factor: 2.826

4.  Evidence for the regulatory function of synaptoplasmic acetyl-CoA in acetylcholine synthesis in nerve endings.

Authors:  H Bielarczyk; A Szutowicz
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

5.  Extending the Scope of 1H NMR Spectroscopy for the Analysis of Cellular Coenzyme A and Acetyl Coenzyme A.

Authors:  G A Nagana Gowda; Lauren Abell; Rong Tian
Journal:  Anal Chem       Date:  2019-01-17       Impact factor: 6.986

6.  Coenzyme A and short-chain acyl-CoA species in control and ischemic rat brain.

Authors:  Joseph Deutsch; Stanley I Rapoport; Thad A Rosenberger
Journal:  Neurochem Res       Date:  2002-12       Impact factor: 3.996

7.  Effect of dichloroacetate on acetyl-CoA content and acetylcholine synthesis in rat brain synaptosomes.

Authors:  A Szutowicz; H Bielarczyk; H Skulimowska
Journal:  Neurochem Res       Date:  1994-09       Impact factor: 3.996

Review 8.  The Regulatory Effects of Acetyl-CoA Distribution in the Healthy and Diseased Brain.

Authors:  Anna Ronowska; Andrzej Szutowicz; Hanna Bielarczyk; Sylwia Gul-Hinc; Joanna Klimaszewska-Łata; Aleksandra Dyś; Marlena Zyśk; Agnieszka Jankowska-Kulawy
Journal:  Front Cell Neurosci       Date:  2018-07-10       Impact factor: 5.505

Review 9.  Acetyl-CoA the key factor for survival or death of cholinergic neurons in course of neurodegenerative diseases.

Authors:  Andrzej Szutowicz; Hanna Bielarczyk; Agnieszka Jankowska-Kulawy; Tadeusz Pawełczyk; Anna Ronowska
Journal:  Neurochem Res       Date:  2013-05-16       Impact factor: 3.996

10.  The Impact of Acetyl-CoA and Aspartate Shortages on the N-Acetylaspartate Level in Different Models of Cholinergic Neurons.

Authors:  Marlena Zyśk; Monika Sakowicz-Burkiewicz; Piotr Pikul; Robert Kowalski; Anna Michno; Tadeusz Pawełczyk
Journal:  Antioxidants (Basel)       Date:  2020-06-13
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.