Literature DB >> 3674373

Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry.

P Dahlén1.   

Abstract

Europium has been used as a label in immunoassays as it can be measured with high sensitivity by means of time-resolved fluorometry. Here we have used streptavidin labeled with europium chelates in the detection of adenovirus type 2 DNA bound to microtiter wells after hybridization with a biotinylated probe. The method gave quantitative results and a sensitivity of about 10 pg of the specific DNA.

Entities:  

Mesh:

Substances:

Year:  1987        PMID: 3674373     DOI: 10.1016/0003-2697(87)90370-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  2 in total

1.  Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid-phase hybridization.

Authors:  P Halonen; E Rocha; J Hierholzer; B Holloway; T Hyypiä; P Hurskainen; M Pallansch
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

2.  Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry.

Authors:  T K Christopoulos; E P Diamandis; G Wilson
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.