Literature DB >> 3670522

Amino acid content in astroglial primary cultures from different brain regions during cultivation.

A Lehmann1, E Hansson.   

Abstract

Free amino acids in astroglial primary cultures obtained from newborn rat cerebral cortex, striatum and hippocampus were analyzed and compared during cultivation. Glutamate and taurine exhibited the highest concentrations. Aspartate and glutamate showed the highest values after 1 and 3 weeks of cultivation with lower values after 2 weeks in culture, while taurine, beta-alanine/hypotaurine and phosphoethanolamine showed the highest value after 2 weeks in culture. The non-neuroactive amino acids and gamma-aminobutyric acid were present at a low level and the former showed the lowest concentration at 2 weeks of cultivation. Astrocytes from the different regions did generally not differ with respect to amino acid content. We conclude that the morphological and biochemical maturation of glia in culture is accompanied with marked quantitative changes in amino acid pattern.

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Year:  1987        PMID： 3670522     DOI： 10.1007/bf00971518

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  19 in total

1.  High-affinity uptake of taurine and beta-alanine in primary cultures of rat astrocytes.

Authors:  I Holopainen; P Kontro
Journal:  Neurochem Res       Date:  1986-02       Impact factor: 3.996

2.  Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures.

Authors:  J Booher; M Sensenbrenner
Journal:  Neurobiology       Date:  1972

3.  Difference in glutamate uptake in astrocytes cultured from different brain regions.

Authors:  A Schousboe; I Divac
Journal:  Brain Res       Date:  1979-11-16       Impact factor: 3.252

4.  A possible interrelationship between extracellular taurine and phosphoethanolamine in the hippocampus.

Authors:  A Lehmann; A Hamberger
Journal:  J Neurochem       Date:  1984-05       Impact factor: 5.372

5.  Amino acid and monoamine transport in primary astroglial cultures from defined brain regions.

Authors:  E Hansson; P Eriksson; M Nilsson
Journal:  Neurochem Res       Date:  1985-10       Impact factor: 3.996

6.  Is there a "dopaminergic glial cell"?

Authors:  E Hansson; L Rönnbäck; A Sellström
Journal:  Neurochem Res       Date:  1984-05       Impact factor: 3.996

7.  Vasoactive intestinal polypeptide receptors linked to an adenylate cyclase, and their relationship with biogenic amine- and somatostatin-sensitive adenylate cyclases on central neuronal and glial cells in primary cultures.

Authors:  H Chneiweiss; J Glowinski; J Prémont
Journal:  J Neurochem       Date:  1985-03       Impact factor: 5.372

8.  Free amino acids of rat astrocytes in primary culture: changes during cell maturation.

Authors:  I Holopainen; S S Oja; K M Marnela; P Kontro
Journal:  Int J Dev Neurosci       Date:  1986       Impact factor: 2.457

9.  Subcellular distribution of neuroactive amino acids in brains of genetically epileptic rats.

Authors:  D W Bonhaus; S E Lippincott; R J Huxtable
Journal:  Epilepsia       Date:  1984-10       Impact factor: 5.864

10.  Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.

Authors:  K D McCarthy; J de Vellis
Journal:  J Cell Biol       Date:  1980-06       Impact factor: 10.539
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  4 in total

1.  Contribution of organic and inorganic osmolytes to volume regulation in rat brain cells in culture.

Authors:  H Pasantes-Morales; S Alavez; R Sánchez Olea; J Morán
Journal:  Neurochem Res       Date:  1993-04       Impact factor: 3.996

2.  Beta-adrenergic receptor regulation, through cyclic AMP, of nerve growth factor expression in rat cortical and cerebellar astrocytes.

Authors:  J P Schwartz; K Mishler
Journal:  Cell Mol Neurobiol       Date:  1990-09       Impact factor: 5.046

Review 3.  Building biosynthetic schools: reviewing compartmentation of CNS taurine synthesis.

Authors:  John Dominy; Stephanie Eller; Ralph Dawson
Journal:  Neurochem Res       Date:  2004-01       Impact factor: 3.996

4.  Metabolic differences between primary cultures of astrocytes and neurons from cerebellum and cerebral cortex. Effects of fluorocitrate.

Authors:  B Hassel; N Westergaard; A Schousboe; F Fonnum
Journal:  Neurochem Res       Date:  1995-04       Impact factor: 3.996
  4 in total

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