Sealed vesicles prepared by fusing Mycoplasma gallisepticum membranes and preformed lipid vesicles.

Sealed vesicles were obtained by fusing Mycoplasma gallisepticum membranes with asolectin-cholesterol vesicles. The fusion was induced by freezing and thawing followed by a brief sonication treatment and was detected using a fluorescence sedimentation behavior in a sucrose density gradient and were shown to be impermeable to small solutes. The advantages of such fused preparations for transport studies in mycoplasmas are discussed.