Literature DB >> 366602

Distribution of yeast fatty acid synthetase subunits: three-dimensional model of the enzyme.

F Wieland, E A Siess, L Renner, C Verfürth, F Lynen.   

Abstract

Rabbit and goat antibodies against the isolated alpha and beta subunits of yeast fatty acid aynthetase were raised and characterized. The purified IgG fractions were studied as to their capability to precipitate their antigens and the holoenzyme and to inhibit the partial reactions involved in overall fatty acyl-CoA synthesis. The specificity of the antibodies was investigated by immunodiffusion and by immunotitration. Native enzyme was crosslinked with each of the antibodies, and dimeric and oligomeric groups of IgG-crosslinked fatty acid synthetase molecules were isolated by sucrose density gradient centrifugation. Electron microscopic investigation of the crosslinked material as well as other data led us to suggest a three-dimensional model of yeast fatty acid synthetase.

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Year:  1978        PMID： 366602      PMCID： PMC393061          DOI： 10.1073/pnas.75.12.5792

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  Biosynthesis of saturated fatty acids.

Authors:  F LYNEN
Journal:  Fed Proc       Date:  1961-12

2.  Reaction of yeast fatty acid synthetase with iodoacetamide. 1. Kinetics of inactivation and extent of carboxamidomethylation.

Authors:  D Oesterhelt; H Bauer; G B Kresze; L Steber; F Lynen
Journal:  Eur J Biochem       Date:  1977-09-15

3.  Localization of the central and peripheral SH-groups on the same polypeptide chain of yeast fatty acid synthetase.

Authors:  G B Kresze; D Oesterhelt; F Lynen; H Castorph; E Schweizer
Journal:  Biochem Biophys Res Commun       Date:  1976-04-19       Impact factor: 3.575

4.  Reaction of yeast fatty acid synthetase with iodoacetamide. 2. Identification of the amino acid residues reacting with iodoacetamide and primary structure of a peptide containing the peripheral sulfhydryl group.

Authors:  G B Kresze; L Steber; D Oesterhelt; F Lynen
Journal:  Eur J Biochem       Date:  1977-09-15

5.  Localization of three non-thiol binding sites on polypeptide chain beta of yeast fatty acid synthetase.

Authors:  H Engeser; F Wieland; F Lynen
Journal:  FEBS Lett       Date:  1977-10-01       Impact factor: 4.124

6.  Pantetheine-free mutants of the yeast fatty-acid-synthetase complex.

Authors:  E Schweizer; B Kniep; H Castorph; U Holzner
Journal:  Eur J Biochem       Date:  1973-11-15

7.  [Malonyl binding site of yeast fatty acid synthetase complex].

Authors:  E Schweizer; F Piccinini; C Duba; S Günther; E Ritter; F Lynen
Journal:  Eur J Biochem       Date:  1970-09

8.  [Small-angle x-ray scattering study of fatty acid synthetase from yeast].

Authors:  I Pilz; M Herbst; O Kratky; D Oesterhelt; F Lynen
Journal:  Eur J Biochem       Date:  1970-03-01

9.  Crystallization of a multienzyme complex: fatty acid synthetase from yeast.

Authors:  D Oesterhelt; H Bauer; F Lynen
Journal:  Proc Natl Acad Sci U S A       Date:  1969-08       Impact factor: 11.205
  9 in total
  5 in total

1.  Structure-function relationships of the yeast fatty acid synthase: negative-stain, cryo-electron microscopy, and image analysis studies of the end views of the structure.

Authors:  J K Stoops; S J Kolodziej; J P Schroeter; J P Bretaudiere; S J Wakil
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

2.  Location of peptide fragments in the fibrinogen molecule by immunoelectron microscopy.

Authors:  J N Telford; J A Nagy; P A Hatcher; H A Scheraga
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

3.  Molecular structure of the multifunctional fatty acid synthetase gene of Brevibacterium ammoniagenes: its sequence of catalytic domains is formally consistent with a head-to-tail fusion of the two yeast genes FAS1 and FAS2.

Authors:  G Meurer; G Biermann; A Schütz; S Harth; E Schweizer
Journal:  Mol Gen Genet       Date:  1992-03

4.  Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase.

Authors:  S Saitoh; K Takahashi; K Nabeshima; Y Yamashita; Y Nakaseko; A Hirata; M Yanagida
Journal:  J Cell Biol       Date:  1996-08       Impact factor: 10.539

5.  Yeast fatty acid synthetase: structure-function relationship and nature of the beta-ketoacyl synthetase site.

Authors:  J K Stoops; S J Wakil
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205
  5 in total

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