Literature DB >> 3663183

A rapid separation method for inositol phosphates and their isomers.

K A Wreggett1, R F Irvine.   

Abstract

A technique is described using ACCELL QMA anion-exchange SEP-PAKs (Waters Associates) with ammonium formate-based solutions, whereby a sample can be processed within minutes to yield resolution of inositol phosphates. Isomers of inositol trisphosphate can then be separated by using this technique in combination with a rapid (5-6 min) isocratic h.p.l.c. procedure. The use of QMA SEP-PAKs offers a degree of reproducibility comparable with that of h.p.l.c. while maintaining the capacity for automation, allowing large numbers of samples to be processed rapidly.

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Year:  1987        PMID： 3663183      PMCID： PMC1148182          DOI： 10.1042/bj2450655

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

1.  Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses.

Authors:  R M DAWSON; N HEMINGTON; J B DAVENPORT
Journal:  Biochem J       Date:  1962-09       Impact factor: 3.857

2.  Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.

Authors:  G Rouser; S Fkeischer; A Yamamoto
Journal:  Lipids       Date:  1970-05       Impact factor: 1.880

3.  A high-performance liquid chromatographic method to measure 32P incorporation into phosphorylated metabolites in cultured cells.

Authors:  E S Sharps; R L McCarl
Journal:  Anal Biochem       Date:  1982-08       Impact factor: 3.365

4.  Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.

Authors:  I R Batty; S R Nahorski; R F Irvine
Journal:  Biochem J       Date:  1985-11-15       Impact factor: 3.857

5.  Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands.

Authors:  M J Berridge; C P Downes; M R Hanley
Journal:  Biochem J       Date:  1982-09-15       Impact factor: 3.857

Review 6.  Inositol trisphosphate, a novel second messenger in cellular signal transduction.

Authors:  M J Berridge; R F Irvine
Journal:  Nature       Date:  1984 Nov 22-28       Impact factor: 49.962

7.  Specificity of inositol trisphosphate-induced calcium release from permeabilized Swiss-mouse 3T3 cells.

Authors:  R F Irvine; K D Brown; M J Berridge
Journal:  Biochem J       Date:  1984-08-15       Impact factor: 3.857

8.  The polyphosphoinositide phosphodiesterase of erythrocyte membranes.

Authors:  C P Downes; R H Michell
Journal:  Biochem J       Date:  1981-07-15       Impact factor: 3.857

9.  Alkaline O leads to N-transacylation. A new method for the quantitative deacylation of phospholipids.

Authors:  N G Clarke; R M Dawson
Journal:  Biochem J       Date:  1981-04-01       Impact factor: 3.857

10.  Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.

Authors:  M J Berridge; R M Dawson; C P Downes; J P Heslop; R F Irvine
Journal:  Biochem J       Date:  1983-05-15       Impact factor: 3.857
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  21 in total

1.  Phosphatidylinositol 4,5-bisphosphate hydrolysis in human sperm stimulated with follicular fluid or progesterone is dependent upon Ca2+ influx.

Authors:  P Thomas; S Meizel
Journal:  Biochem J       Date:  1989-12-01       Impact factor: 3.857

2.  Synthesis of polyphosphoinositides in nuclei of Friend cells. Evidence for polyphosphoinositide metabolism inside the nucleus which changes with cell differentiation.

Authors:  L Cocco; R S Gilmour; A Ognibene; A J Letcher; F A Manzoli; R F Irvine
Journal:  Biochem J       Date:  1987-12-15       Impact factor: 3.857

3.  Inositol 1,4,5-trisphosphorothioate, a stable analogue of inositol trisphosphate which mobilizes intracellular calcium.

Authors:  C W Taylor; M J Berridge; A M Cooke; B V Potter
Journal:  Biochem J       Date:  1989-05-01       Impact factor: 3.857

4.  A novel metal-dye detection system permits picomolar-range h.p.l.c. analysis of inositol polyphosphates from non-radioactively labelled cell or tissue specimens.

Authors:  G W Mayr
Journal:  Biochem J       Date:  1988-09-01       Impact factor: 3.857

5.  Extraction and recovery of inositol phosphates from tissues.

Authors:  K A Wreggett; L R Howe; J P Moore; R F Irvine
Journal:  Biochem J       Date:  1987-08-01       Impact factor: 3.857

6.  Phosphatidylinositol 4,5-bisphosphate phosphodiesterase in higher plants.

Authors:  W C McMurray; R F Irvine
Journal:  Biochem J       Date:  1988-02-01       Impact factor: 3.857

7.  Roles of threonine 192 and asparagine 382 in agonist and antagonist interactions with M1 muscarinic receptors.

Authors:  X P Huang; P I Nagy; F E Williams; S M Peseckis; W S Messer
Journal:  Br J Pharmacol       Date:  1999-02       Impact factor: 8.739

8.  Phosphoinositides in Barley (Hordeum vulgare L.) Aleurone Tissue.

Authors:  C. A. Brearley; D. E. Hanke
Journal:  Plant Physiol       Date:  1994-04       Impact factor: 8.340

9.  The phosphoinositide signaling cycle in myelin requires cooperative interaction with the axon.

Authors:  G Chakraborty; A Drivas; R Ledeen
Journal:  Neurochem Res       Date:  1999-02       Impact factor: 3.996

10.  Construction of a novel bifunctional biogenic amine receptor by two point mutations of the H2-histamine receptor.

Authors:  J DelValle; I Gantz; L Wang; Y J Guo; G Munzert; T Tashiro; Y Konda; T Yamada
Journal:  Mol Med       Date:  1995-03       Impact factor: 6.354
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