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Literature DB >> 3662826

Cell type specific expression of pre S 1 antigen and secretion of hepatitis B virus surface antigen. Brief Report.

O Marquardt¹, K H Heermann, M Seifer, W H Gerlich.

Abstract

Production of the three hepatitis B surface (HBs) proteins was studied in a hepatoma cell line (PLC/PRF/5) and two HBs antigen secreting cell lines (HeLa and mouse L-cells), which had been transfected by a viral genome isolated by molecular cloning from PLC/PRF/5 chromosomal DNA. The DNA used for transfection contains the HBs-specific promoters and the enhancer which regulate the expression of HBs genes in the transfected cell lines. All three cell lines expressed well the small and middle HBs protein, but the larger pre S 1 containing protein was barely detectable in the L-cell. In vivo growth of the transfected HeLa cell as nude mouse tumour increased pre S 1 expression and suppressed secretion of HBsAg.

Entities: Disease Gene Species

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Year: 1987 PMID： 3662826 DOI： 10.1007/bf01320964

Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574

24 in total

1. Expression of hepatitis B virus large envelope polypeptide inhibits hepatitis B surface antigen secretion in transgenic mice.

Authors: F V Chisari; P Filippi; A McLachlan; D R Milich; M Riggs; S Lee; R D Palmiter; C A Pinkert; R L Brinster
Journal: J Virol Date: 1986-12 Impact factor: 5.103

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. Demonstration of pre-S polypeptides of hepatitis B virus in infected livers.

Authors: S N Thung; M A Gerber; E J Kasambalides; B K Gilja; W Keh; W H Gerlich
Journal: Hepatology Date: 1986 Nov-Dec Impact factor: 17.425

4. Large surface proteins of hepatitis B virus containing the pre-s sequence.

Authors: K H Heermann; U Goldmann; W Schwartz; T Seyffarth; H Baumgarten; W H Gerlich
Journal: J Virol Date: 1984-11 Impact factor: 5.103

5. Core and E antigen synthesis in rodent cells transformed with hepatitis B virus DNA is associated with greater than genome length viral messenger RNAs.

Authors: N M Gough
Journal: J Mol Biol Date: 1983-04-25 Impact factor: 5.469

6. Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus.

Authors: A R Neurath; S B Kent; N Strick; K Parker
Journal: Cell Date: 1986-08-01 Impact factor: 41.582

7. Signals regulating hepatitis B surface antigen transcription.

Authors: R Cattaneo; H Will; N Hernandez; H Schaller
Journal: Nature Date: 1983 Sep 22-28 Impact factor: 49.962

8. Transcription of the hepatitis B surface antigen gene in cultured murine cells initiates within the presurface region.

Authors: D N Standring; W J Rutter; H E Varmus; D Ganem
Journal: J Virol Date: 1984-05 Impact factor: 5.103

Cell type specific expression of pre S 1 antigen and secretion of hepatitis B virus surface antigen. Brief Report.

1. Expression of hepatitis B virus large envelope polypeptide inhibits hepatitis B surface antigen secretion in transgenic mice.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. Demonstration of pre-S polypeptides of hepatitis B virus in infected livers.

4. Large surface proteins of hepatitis B virus containing the pre-s sequence.

5. Core and E antigen synthesis in rodent cells transformed with hepatitis B virus DNA is associated with greater than genome length viral messenger RNAs.

6. Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus.

7. Signals regulating hepatitis B surface antigen transcription.

8. Transcription of the hepatitis B surface antigen gene in cultured murine cells initiates within the presurface region.

9. Co-transcribed 3' host sequences augment expression of integrated hepatitis B virus DNA.

10. Hepatitis B surface antigen produced by a human hepatoma cell line.

1. Increased growth of permanent mouse fibroblasts in soft agar after transfection with hepatitis B virus DNA.

2. High density culture of HeLa cells in a CelliGen perfusion system.

3. Expression of large hepatitis B envelope protein mutants using a new expression vector.

Review 4. Quantification of HBsAg: basic virology for clinical practice.

Review 5. Prophylactic vaccination against hepatitis B: achievements, challenges and perspectives.

6. Malignant transformation of immortalized transgenic hepatocytes after transfection with hepatitis B virus DNA.