Literature DB >> 3654948

Determination of hippurate hydrolysis by gas-liquid chromatography.

P L Wallace1, C M Patton, C W Moss.   

Abstract

A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37 degrees C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive, and its specificity provides a high degree of reliability for organisms with weak hippuricase activity.

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Year:  1987        PMID： 3654948      PMCID： PMC269324          DOI： 10.1128/jcm.25.9.1766-1768.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Rapid hippurate hydrolysis method for presumptive identification of group B streptococci.

Authors:  M N Hwang; G M Ederer
Journal:  J Clin Microbiol       Date:  1975-01       Impact factor: 5.948

2.  Hippurate hydrolysis by Campylobacter fetus.

Authors:  S M Harvey
Journal:  J Clin Microbiol       Date:  1980-04       Impact factor: 5.948

3.  Rapid, colorimetric test for the determination of hippurate hydrolysis by group B Streptococcus.

Authors:  S C Edberg; S Samuels
Journal:  J Clin Microbiol       Date:  1976-01       Impact factor: 5.948

4.  Gas-liquid chromatography technique for detection of hippurate hydrolysis and conversion of fumarate to succinate by microorganisms.

Authors:  H Kodaka; G L Lombard; V R Dowell
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948

5.  Comparison of four hippurate hydrolysis methods for identification of thermophilic Campylobacter spp.

Authors:  G K Morris; M R el Sherbeeny; C M Patton; H Kodaka; G L Lombard; P Edmonds; D G Hollis; D J Brenner
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948

6.  Two-dimensional thin-layer chromatography for the specific detection of hippurate hydrolysis by microorganisms.

Authors:  J Y Lin; K C Chen; J Hale; P A Totten; K K Holmes
Journal:  J Clin Microbiol       Date:  1986-01       Impact factor: 5.948

7.  DNA relatedness among strains of Campylobacter jejuni and Campylobacter coli with divergent serogroup and hippurate reactions.

Authors:  G A Hébert; P Edmonds; D J Brenner
Journal:  J Clin Microbiol       Date:  1984-07       Impact factor: 5.948
  7 in total
  5 in total

1.  Evaluation of a disk method for detection of hippurate hydrolysis by Campylobacter spp.

Authors:  J B Cacho; P M Aguirre; A Hernanz; A C Velasco
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948

2.  Detection of hippurate hydrolysis by Legionella spp. by using a rapid high-performance liquid chromatography method.

Authors:  D Marmet; N Bornstein; J Fleurette
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

3.  Evaluation of disk method for hippurate hydrolysis by Campylobacter species.

Authors:  M A Nicholson; C M Patton
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

4.  Prevalence and characterization of hippurate-negative Campylobacter jejuni in King County, Washington.

Authors:  P A Totten; C M Patton; F C Tenover; T J Barrett; W E Stamm; A G Steigerwalt; J Y Lin; K K Holmes; D J Brenner
Journal:  J Clin Microbiol       Date:  1987-09       Impact factor: 5.948

5.  Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli.

Authors:  E K Hani; V L Chan
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490
  5 in total

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