| Literature DB >> 3654825 |
Abstract
Adenovirus (Type 2)-infected HeLa cells were sonicated and treated with 1,1,2-trichlorotrifluoroethane. The water-soluble extract was ultracentrifuged and the supernatant, containing the dissociated proteins, was subjected to anion-exchange high-performance liquid chromatography on a Mono Q column in 50 mM bis-Tris-HCl (pH 6.5). Elution with a linear salt gradient resulted in a major peak, containing the hexon protein.Entities:
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Year: 1987 PMID： 3654825 DOI： 10.1016/s0021-9673(01)85015-9
Source DB: PubMed Journal: J Chromatogr