Literature DB >> 3652820

Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs.

G Harauz1, L Borland, G F Bahr, E Zeitler, M van Heel.   

Abstract

A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was fairly homogeneous within the chromatids and neither a hollow nor a dense core was observed. The distribution and course of fibers observed are most consistent with a looping model of chromosome structure.

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Year:  1987        PMID： 3652820     DOI： 10.1007/BF00293184

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  24 in total

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Authors:  G F BAHR; E ZEITLER
Journal:  Lab Invest       Date:  1965-06       Impact factor: 5.662

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Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

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Authors:  H M Golomb; G F Bahr
Journal:  Science       Date:  1971-03-12       Impact factor: 47.728

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Authors:  K W Adolph; L R Kreisman; R L Kuehn
Journal:  Biophys J       Date:  1986-01       Impact factor: 4.033

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Authors:  G Harauz; F P Ottensmeyer
Journal:  Science       Date:  1984-11-23       Impact factor: 47.728

6.  Electron microscope tomography: transcription in three dimensions.

Authors:  D E Olins; A L Olins; H A Levy; R C Durfee; S M Margle; E P Tinnel; S D Dover
Journal:  Science       Date:  1983-04-29       Impact factor: 47.728

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Authors:  K W Adolph
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316

Review 8.  Nucleosome structure.

Authors:  J D McGhee; G Felsenfeld
Journal:  Annu Rev Biochem       Date:  1980       Impact factor: 23.643

9.  High-order structure of metaphase chromosomes: evidence for a multiple coiling model.

Authors:  T Taniguchi; S Takayama
Journal:  Chromosoma       Date:  1986       Impact factor: 4.316

10.  Cryo-electron microscopy of vitrified chromosomes in situ.

Authors:  A W McDowall; J M Smith; J Dubochet
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598
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  8 in total

1.  Chromosome inner structure investigation by electron tomography and electron diffraction in a transmission electron microscope.

Authors:  Rinyaporn Phengchat; Marek Malac; Misa Hayashida
Journal:  Chromosome Res       Date:  2021-03-18       Impact factor: 5.239

2.  Packing of the 30 nm chromatin fiber in the human metaphase chromosome.

Authors:  L Borland; G Harauz; G Bahr; M van Heel
Journal:  Chromosoma       Date:  1988       Impact factor: 4.316

3.  The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography.

Authors:  Peter König; Michael B Braunfeld; John W Sedat; David A Agard
Journal:  Chromosoma       Date:  2007-02-28       Impact factor: 2.919

4.  Lamin B distribution and association with peripheral chromatin revealed by optical sectioning and electron microscopy tomography.

Authors:  A S Belmont; Y Zhai; A Thilenius
Journal:  J Cell Biol       Date:  1993-12       Impact factor: 10.539

Review 5.  Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes.

Authors:  Mohammed Yusuf; Kohei Kaneyoshi; Kiichi Fukui; Ian Robinson
Journal:  Chromosoma       Date:  2018-09-03       Impact factor: 4.316

6.  Full-length human cytomegalovirus terminase pUL89 adopts a two-domain structure specific for DNA packaging.

Authors:  Janine Theiß; Min Woo Sung; Andreas Holzenburg; Elke Bogner
Journal:  PLoS Pathog       Date:  2019-12-06       Impact factor: 6.823

7.  Visualization of G1 chromosomes: a folded, twisted, supercoiled chromonema model of interphase chromatid structure.

Authors:  A S Belmont; K Bruce
Journal:  J Cell Biol       Date:  1994-10       Impact factor: 10.539

8.  Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.

Authors:  Hanfei Yan; Evgeny Nazaretski; Kenneth Lauer; Xiaojing Huang; Ulrich Wagner; Christoph Rau; Mohammed Yusuf; Ian Robinson; Sebastian Kalbfleisch; Li Li; Nathalie Bouet; Juan Zhou; Ray Conley; Yong S Chu
Journal:  Sci Rep       Date:  2016-02-05       Impact factor: 4.379
  8 in total

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