Dynamics of cancer cell interactions with microvasculature and interstitium.

Interactions of cancer cells with the microvasculature and the interstitium of non-malignant tissue were studied in a rabbit ear chamber preparation using intravital fluorescent microscopy. Injection of VX2 carcinoma cells into the auricular artery feeding the chamber led to mechanical entrapment, adhesion, and in some instances, extravasation of cancer cells. Implantation of VX2 cells in the interstitial space led to increases in the interstitial diffusion coefficients and the microvascular permeability. Our results are compared with those available in literature and directions for future research are pointed out.