Literature DB >> 364473

A photo-CIDNP study of the interaction of oligonucleotides with gene-5 protein of bacteriophage M13.

G J Garssen, R Kaptein, J G Schoenmakers, C W Hilbers.   

Abstract

It is shown that photo-CIDNP effects (CIDNP, chemically induced dynamic nuclear polarization) can be generated in the 360-MHz proton NMR spectrum of gene-5 protein from bacteriophage M13. This technique is used to determine the number of tyrosyl residues at the surface of the protein and to assign the resonances from the 3,5-ring protons of these residues. The DNA-binding site of the protein is investigated by formation of complexes with oligonucleotides. Complex formation leads to shifting and/or quenching of the photo-CIDNP emission signals of the surface tyrosines, implying that they are involved in DNA-protein interaction. These experiments are complemented by studying the complex formation of Lys-Tyr-Lys to poly(A).

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Year:  1978        PMID： 364473      PMCID： PMC392946          DOI： 10.1073/pnas.75.11.5281

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  Circular dichroism and ultraviolet absorption of a deoxyribonucleic acid binding protein of filamentous bacteriophage.

Authors:  L A Day
Journal:  Biochemistry       Date:  1973-12-18       Impact factor: 3.162

2.  Isolation and characterization of gene 5 protein of filamentous bacterial viruses.

Authors:  B Alberts; L Frey; H Delius
Journal:  J Mol Biol       Date:  1972-07-14       Impact factor: 5.469

3.  Interaction of oligopeptides containing aromatic amino acids with nucleic acids. Fluorescence and proton magnetic resonance studies.

Authors:  C Helene; J L Dimicoli
Journal:  FEBS Lett       Date:  1972-10-01       Impact factor: 4.124

4.  Laser photo-CIDNP as a surface probe for proteins in solution.

Authors:  R Kaptein; K Dijkstra; K Nicolay
Journal:  Nature       Date:  1978-07-20       Impact factor: 49.962

5.  Interaction of aromatic residues of proteins with nucleic acids. Circular dichroism studies of the binding of oligopeptides to poly(adenylic acid).

Authors:  M Durand; J C Maurizot; H N Borazan; C Hélène
Journal:  Biochemistry       Date:  1975-02-11       Impact factor: 3.162

6.  Interactions of aromatic residues of proteins with nucleic acids. Fluorescence studies of the binding of oligopeptides containing tryptophan and tyrosine residues to polynucleotides.

Authors:  F Brun; J J Toulmé; C Hélène
Journal:  Biochemistry       Date:  1975-02-11       Impact factor: 3.162

7.  Gene V protein of fd bacteriophage. Dimer formation and the role of tyrosyl groups in DNA binding.

Authors:  H T Pretorius; M Klein; L A Day
Journal:  J Biol Chem       Date:  1975-12-25       Impact factor: 5.157

8.  Chemical modifications of functional residues of fd gene 5 DNA-binding protein.

Authors:  R A Anderson; Y Nakashima; J E Coleman
Journal:  Biochemistry       Date:  1975-03-11       Impact factor: 3.162

9.  Studies on DNA unwinding. Proton and phosphorus nuclear-magnetic-resonance studies of gene V protein from bacteriophage M13, interacting with d(pC-G-C-G).

Authors:  G J Garssen; C W Hilbers; J G Schoenmakers; J H van Boom
Journal:  Eur J Biochem       Date:  1977-12

10.  Structure of gene 5 protein-oligodeoxynucleotide complexes as determined by 1H, 19F, and 31P nuclear magnetic resonance.

Authors:  J E Coleman; R A Anderson; R G Ratcliffe; I M Armitage
Journal:  Biochemistry       Date:  1976-12-14       Impact factor: 3.162
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  1 in total

1.  Photochemically induced dynamic nuclear polarization investigation of complex formation of the NH2-terminal DNA-binding domain of lac repressor with poly[d(AT)].

Authors:  F Buck; H Rüterjans; R Kaptein; K Beyreuther
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205
  1 in total

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