Literature DB >> 3638992

Purification and characterization of pancreatic secretory trypsin inhibitor in human gastric mucosa.

T Shibata, M Ogawa, K Matsuda, K Miyauchi, T Yamamoto, T Mori.   

Abstract

Immunoreactive pancreatic secretory trypsin inhibitor (PSTI) was purified to homogeneity from human gastric mucosa by gel chromatography, ion-exchange chromatography, and repeated reverse-phase high-performance liquid chromatography (HPLC). The molecular weight of the purified immunoreactive PSTI in human gastric mucosa was estimated to be 6000. The electrophoretical mobility of purified PSTI was identical with that of the main component of PSTI in human pancreatic juice. It consisted of 56 amino acids and had the same amino acid composition as PSTI in pancreatic juice. It inhibited bovine pancreatic trypsin stoichiometrically, and did not inhibit porcine pancreatic kallikrein or elastase. Heat treatment of immunoreactive PSTI in gastric mucosa showed the same inactivation curve of immunoreactivity as that of pancreatic juice PSTI.

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Year:  1986        PMID： 3638992     DOI： 10.1016/0009-8981(86)90163-4

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  7 in total

1.  Immunoreactive pancreatic secretory trypsin inhibitor in normal, inflammatory and neoplastic gallbladders.

Authors:  H Bohe; M Bohe; C Lindström; K Ohlsson
Journal:  Gastroenterol Jpn       Date:  1991-02

2.  Production and secretion of pancreatic secretory trypsin inhibitor in normal human small intestine.

Authors:  H Bohe; M Bohe; E Lundberg; A Polling; K Ohlsson
Journal:  J Gastroenterol       Date:  1997-10       Impact factor: 7.527

3.  Immunohistochemical demonstration of pancreatic secretory trypsin inhibitor in normal and neoplastic colonic mucosa.

Authors:  H Bohe; M Bohe; C Lindström; K Ohlsson
Journal:  J Clin Pathol       Date:  1990-11       Impact factor: 3.411

4.  Isolation and characterization of a 60-residue intestinal peptide structurally related to the pancreatic secretory type of trypsin inhibitor: influence on insulin secretion.

Authors:  B Agerberth; J Söderling-Barros; H Jörnvall; Z W Chen; C G Ostenson; S Efendić; V Mutt
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

5.  Pancreatic secretory trypsin inhibitor in gastrointestinal mucosa and gastric juice.

Authors:  T C Freeman; R J Playford; C Quinn; K Beardshall; L Poulter; J Young; J Calam
Journal:  Gut       Date:  1990-11       Impact factor: 23.059

6.  Expression pattern of serine protease inhibitor kazal type 3 (Spink3) during mouse embryonic development.

Authors:  Jun Wang; Masaki Ohmuraya; Masahiko Hirota; Hideo Baba; Gang Zhao; Motohiro Takeya; Kimi Araki; Ken-ichi Yamamura
Journal:  Histochem Cell Biol       Date:  2008-04-02       Impact factor: 4.304

7.  Quantification of pancreatic secretory trypsin inhibitor in colonic carcinoma and normal adjacent colonic mucosa.

Authors:  H Bohe; M Bohe; P Jönsson; C Lindström; K Ohlsson
Journal:  J Clin Pathol       Date:  1992-12       Impact factor: 3.411
  7 in total

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