Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland.

Literature DB >> 3636812

The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland.

Abstract

Mouse submaxillary gland contains many proteolytic enzymes, the most widely studied of which are the kallikreins. This gland also contains high levels of nerve growth factor (NGF), which is isolated as a complex of three subunits, alpha, beta, and gamma. We report here the cloning and sequence analysis of a novel kallikrein from mouse submaxillary gland. Antibodies directed against the alpha subunit precipitate the product of this clone, but do not precipitate the homologous gamma subunit. This new kallikrein is therefore closely related to alpha NGF, yet in contrast to the alpha subunit, its sequence suggests it has proteolytic activity.

Entities: Gene Species

Mesh：

Substances：

Year: 1986 PMID： 3636812 PMCID： PMC311494 DOI： 10.1093/nar/14.12.4823

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

28 in total

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors: M Grunstein; D S Hogness
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

2. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

3. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors: R D Palmiter
Journal: Biochemistry Date: 1974-08-13 Impact factor: 3.162

The sequence of a cDNA clone coding for a novel kallikrein from mouse submaxillary gland.

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

2. A new method for sequencing DNA.

3. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

4. Multiple forms of the nerve growth factor protein and its subunits.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Comparison of the arginine esteropeptidases associated with the nerve and epidermal growth factor.

7. Enzymatic in vitro synthesis of globin genes.

8. Evidence for pro-beta-nerve growth factor, a biosynthetic precursor to beta-nerve growth factor.

9. Growth factor-binding proteases in the murine submaxillary gland: isolation of a cDNA clone.

10. Genes for the alpha and gamma subunits of mouse nerve growth factor are contiguous.

1. The NGF complex from the African rat Mastomys natalensis.