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Literature DB >> 363446

Dithiols simulate endotoxin in the Limulus reaction.

Abstract

Dithiothreitol, dithioerythritol and bacterial lipopolysaccharides increase optical absorbance and clot Limulus lysate. Purification of dithiothreitol from possible endotoxin contamination by vacuum sublimation or chromatography does not abolish the reaction with lysate. The dithiols reported active here represent the smallest molecules capable of simulating endotoxin in the Limulus test.

Entities: Chemical

Mesh：

Substances：

Year: 1978 PMID： 363446 DOI： 10.1007/bf01922927

Source DB: PubMed Journal: Experientia ISSN： 0014-4754

17 in total

Review 1. Biology of endotoxin.

Authors: R J Elin; S M Wolff
Journal: Annu Rev Med Date: 1976 Impact factor: 13.739

2. Nonspecificity of the limulus amebocyte lysate test: positive reactions with polynucleotides and proteins.

Authors: R J Elin; S M Wolff
Journal: J Infect Dis Date: 1973-09 Impact factor: 5.226

3. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria.

Authors: E T Yin; C Galanos; S Kinsky; R A Bradshaw; S Wessler; O Lüderitz; M E Sarmiento
Journal: Biochim Biophys Acta Date: 1972-01-28

1. Interaction between limulus amoebocyte lysate and soluble antigens from Pseudomonas aeruginosa and Staphylococcus aureus studied by quantitative immunoelectrophoresis.

Authors: L Baek; N Høiby; J B Hertz; F Espersen
Journal: J Clin Microbiol Date: 1985-08 Impact factor: 5.948

1 in total

Dithiols simulate endotoxin in the Limulus reaction.

Review 1. Biology of endotoxin.

2. Nonspecificity of the limulus amebocyte lysate test: positive reactions with polynucleotides and proteins.

3. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria.

4. A technique for quantitative measurement of endotoxin in human plasma.

5. The Limulus coagulation test for endotoxin. A comparison with other assay methods.

6. A sensitive spectrophotometric method for measurement of plasma endotoxin.

7. Rapid detection of gram-negative bacterial meningitis by the limulus lysate test.

8. Rapid detection of gram-negative bacteriuria by use of the Limulus endotoxin assay.

9. An invertebrate coagulation system activated by endotoxin: evidence for enzymatic mediation.

10. Investigations on the specificity of the Limulus test for the detection of endotoxin.

1. Interaction between limulus amoebocyte lysate and soluble antigens from Pseudomonas aeruginosa and Staphylococcus aureus studied by quantitative immunoelectrophoresis.