Literature DB >> 36272086

A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.

Konstantinos Sousounis1, Katharine Courtemanche1, Jessica L Whited2,3,4.   

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Axolotl screening; Axolotls; CRISPR; CRISPR design; CRISPR-Cas9; Genome targeting

Year:  2023        PMID： 36272086     DOI： 10.1007/978-1-0716-2659-7_22

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

1.  Basic local alignment search tool.

Authors:  S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
Journal:  J Mol Biol       Date:  1990-10-05       Impact factor: 5.469

2.  Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease.

Authors:  G Parker Flowers; Andrew T Timberlake; Kaitlin C McLean; James R Monaghan; Craig M Crews
Journal:  Development       Date:  2014-04-24       Impact factor: 6.868

3.  Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum).

Authors:  Ji-Feng Fei; Wilson Pak-Kin Lou; Dunja Knapp; Prayag Murawala; Tobias Gerber; Yuka Taniguchi; Sergej Nowoshilow; Shahryar Khattak; Elly M Tanaka
Journal:  Nat Protoc       Date:  2018-12       Impact factor: 13.491

4.  A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

Authors:  Martin Jinek; Krzysztof Chylinski; Ines Fonfara; Michael Hauer; Jennifer A Doudna; Emmanuelle Charpentier
Journal:  Science       Date:  2012-06-28       Impact factor: 47.728

5.  Eya2 promotes cell cycle progression by regulating DNA damage response during vertebrate limb regeneration.

Authors:  Konstantinos Sousounis; Donald M Bryant; Jose Martinez Fernandez; Samuel S Eddy; Stephanie L Tsai; Gregory C Gundberg; Jihee Han; Katharine Courtemanche; Michael Levin; Jessica L Whited
Journal:  Elife       Date:  2020-03-06       Impact factor: 8.140

6.  Detouring the roadblocks in gene expression.

Authors:  Jan Philipp Junker
Journal:  Nat Rev Mol Cell Biol       Date:  2019-04       Impact factor: 94.444

7.  Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration.

Authors:  Ji-Feng Fei; Maritta Schuez; Dunja Knapp; Yuka Taniguchi; David N Drechsel; Elly M Tanaka
Journal:  Proc Natl Acad Sci U S A       Date:  2017-10-31       Impact factor: 11.205

8.  Lineage tracing of genome-edited alleles reveals high fidelity axolotl limb regeneration.

Authors:  Grant Parker Flowers; Lucas D Sanor; Craig M Crews
Journal:  Elife       Date:  2017-09-16       Impact factor: 8.140

9.  Multiplex CRISPR/Cas screen in regenerating haploid limbs of chimeric Axolotls.

Authors:  Lucas D Sanor; Grant Parker Flowers; Craig M Crews
Journal:  Elife       Date:  2020-01-28       Impact factor: 8.140

10.  CRISPR-mediated genomic deletion of Sox2 in the axolotl shows a requirement in spinal cord neural stem cell amplification during tail regeneration.

Authors:  Ji-Feng Fei; Maritta Schuez; Akira Tazaki; Yuka Taniguchi; Kathleen Roensch; Elly M Tanaka
Journal:  Stem Cell Reports       Date:  2014-08-07       Impact factor: 7.765
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