Literature DB >> 36272085

Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System.

Maritta Schuez1, Tatiana Sandoval-Guzmán2.   

Abstract

The axolotl (Ambystoma mexicanum ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl transgenesis using either I-SceI meganuclease or the mini Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Axolotl eggs; Egg injection; I-SceI meganuclease; Transgenesis; mTol2 transposase

Year:  2023        PMID： 36272085     DOI： 10.1007/978-1-0716-2659-7_21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  I-SceI meganuclease-mediated transgenesis in Xenopus.

Authors:  Fong Cheng Pan; Yonglong Chen; Jana Loeber; Kristine Henningfeld; Tomas Pieler
Journal:  Dev Dyn       Date:  2006-01       Impact factor: 3.780

2.  Generation of transgenic newt Cynops pyrrhogaster for regeneration study.

Authors:  Yoko Ueda; Hisato Kondoh; Nobuhiko Mizuno
Journal:  Genesis       Date:  2005-02       Impact factor: 2.487

3.  Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination.

Authors:  Shahryar Khattak; Prayag Murawala; Heino Andreas; Verena Kappert; Maritta Schuez; Tatiana Sandoval-Guzmán; Karen Crawford; Elly M Tanaka
Journal:  Nat Protoc       Date:  2014-02-06       Impact factor: 13.491

4.  Transposon-mediated BAC transgenesis in zebrafish.

Authors:  Maximiliano L Suster; Gembu Abe; Anders Schouw; Koichi Kawakami
Journal:  Nat Protoc       Date:  2011-12-01       Impact factor: 13.491

5.  A germline GFP transgenic axolotl and its use to track cell fate: dual origin of the fin mesenchyme during development and the fate of blood cells during regeneration.

Authors:  Lidia Sobkow; Hans-Henning Epperlein; Stephan Herklotz; Werner L Straube; Elly M Tanaka
Journal:  Dev Biol       Date:  2006-01-04       Impact factor: 3.582

6.  I-SceI meganuclease mediates highly efficient transgenesis in fish.

Authors:  Violette Thermes; Clemens Grabher; Filomena Ristoratore; Franck Bourrat; André Choulika; Jochen Wittbrodt; Jean-Stéphane Joly
Journal:  Mech Dev       Date:  2002-10       Impact factor: 1.882

7.  Fundamental differences in dedifferentiation and stem cell recruitment during skeletal muscle regeneration in two salamander species.

Authors:  Tatiana Sandoval-Guzmán; Heng Wang; Shahryar Khattak; Maritta Schuez; Kathleen Roensch; Eugeniu Nacu; Akira Tazaki; Alberto Joven; Elly M Tanaka; András Simon
Journal:  Cell Stem Cell       Date:  2013-11-21       Impact factor: 24.633

8.  Germline transgenic methods for tracking cells and testing gene function during regeneration in the axolotl.

Authors:  Shahryar Khattak; Maritta Schuez; Tobias Richter; Dunja Knapp; Saori L Haigo; Tatiana Sandoval-Guzmán; Kristyna Hradlikova; Annett Duemmler; Ryan Kerney; Elly M Tanaka
Journal:  Stem Cell Reports       Date:  2013-06-04       Impact factor: 7.765

9.  Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools.

Authors:  Joshua D Currie; Akane Kawaguchi; Ricardo Moreno Traspas; Maritta Schuez; Osvaldo Chara; Elly M Tanaka
Journal:  Dev Cell       Date:  2016-11-10       Impact factor: 12.270

10.  Transposon-mediated BAC transgenesis in zebrafish and mice.

Authors:  Maximiliano L Suster; Kenta Sumiyama; Koichi Kawakami
Journal:  BMC Genomics       Date:  2009-10-16       Impact factor: 3.969
  10 in total

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