Literature DB >> 36272076

COMET Assay for Detection of DNA Damage During Axolotl Tail Regeneration.

Belfran Carbonell1,2, Jennifer Álvarez1, Gloria A Santa-González1,3, Jean Paul Delgado4.   

Abstract

The purpose of this chapter is to evaluate DNA damage during axolotl tail regeneration using an alkaline comet assay. Our method details the isolation of cells from regenerating and non-regenerating tissues and the isolation of peripheral blood for single-cell gel electrophoresis. Also, we detail each of the steps for the development of the comet assay technique which includes mounting the isolated cells on an agarose matrix, alkaline electrophoresis, and DNA damage detection.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Alkaline comet assay; DNA damage; Tail regeneration; single-cell gel electrophoresis

Year:  2023        PMID： 36272076     DOI： 10.1007/978-1-0716-2659-7_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Amputation-induced reactive oxygen species signaling is required for axolotl tail regeneration.

Authors:  Nour W Al Haj Baddar; Adarsh Chithrala; S Randal Voss
Journal:  Dev Dyn       Date:  2018-12-21       Impact factor: 3.780

2.  Culture and transfection of axolotl cells.

Authors:  Jean-François Denis; Fadi Sader; Patrizia Ferretti; Stéphane Roy
Journal:  Methods Mol Biol       Date:  2015

3.  A simple technique for quantitation of low levels of DNA damage in individual cells.

Authors:  N P Singh; M T McCoy; R R Tice; E L Schneider
Journal:  Exp Cell Res       Date:  1988-03       Impact factor: 3.905

4.  Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells.

Authors:  O Ostling; K J Johanson
Journal:  Biochem Biophys Res Commun       Date:  1984-08-30       Impact factor: 3.575

5.  Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest.

Authors:  Gloria A Santa-Gonzalez; Andrea Gomez-Molina; Mauricio Arcos-Burgos; Joel N Meyer; Mauricio Camargo
Journal:  Redox Biol       Date:  2016-07-21       Impact factor: 11.799

6.  OpenComet: an automated tool for comet assay image analysis.

Authors:  Benjamin M Gyori; Gireedhar Venkatachalam; P S Thiagarajan; David Hsu; Marie-Veronique Clement
Journal:  Redox Biol       Date:  2014-01-09       Impact factor: 11.799
  6 in total

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