Bishnu Maya Bashyal1, Kirti Rawat2, Pooja Parmar2, Ashish Kumar Gupta3, Sangeeta Gupta2, S Gopala Krishnan4, Ravish Choudhary5, Sezai Ercisli6, Antonija Kovacevic7, Rashmi Aggarwal2. 1. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India. bishnumayabashyal@gmail.com. 2. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India. 3. ICAR-National Institute of Plant Biotechnology, 110012, New Delhi, India. 4. Division of Genetics, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India. 5. Division of Seed Science and Technology, Indian Agricultural Research Institute, 110012, New Delhi, India. 6. Department of Horticulture, Agricultural Faculty, Ataturk University, 25240, Erzurum, Turkey. 7. BIOTECH - Center for Biotechnological Research and Development, Ivana Cankara 76, 35 000, Slavonski Brod, Croatia.
Abstract
BACKGROUND: Fusarium fujikuroi causing bakanae is one of the most significant pathogens of rice and much responsible for yield losses thereby emerging as a major risk to food security. METHODS: In the present study transcriptomic analysis was conducted between two contrasting resistant (C101A51) and susceptible (Rasi) genotypes of rice with the combinations of C101A51 control (CC) vs. C101A51 inoculated (CI); Rasi control (RC) vs. Rasi inoculated (RI) and C101A51 inoculated (CI) vs. Rasi inoculated (RI). RESULTS: In CC vs. CI commonly expressed genes were 12,764. Out of them 567 (4%) were significantly upregulated and 1399 (9%) genes were downregulated. For the RC vs. RI 14, 333 (79%) genes were commonly expressed. For CI vs. RI 13,662 (72%) genes were commonly expressed. Genes related to cysteine proteinase inhibitor 10, disease resistance protein TAO1-like, oleosin 16 kDa-like, pathogenesis-related protein (PR1), (PR4), BTB/POZ and MATH domain-containing protein 5-like, alpha-amylase isozyme were upregulated in resistant genotype C101A51. Whereas, genes related to GDSL esterase/lipase, serine glyoxylate aminotransferase, CASP-like protein 2C1, WAT1-related protein, Cytoplasmic linker associated proteins, xyloglucan endotransglucosylase/hydrolase protein and β-D xylosidase 7 were upregulated in susceptible genotype Rasi. Gene ontology analysis showed functions related to defence response (GO:0006952), regulation of plant hypersensitive type response (GO:0010363), Potassium ion transmembrane activity (GO:0015079), chloroplast (GO:0009507), response to wounding (GO:0009611), xylan biosynthetic process (GO:0045492) were upregulated in resistant genotype C101A51 under inoculated conditions. CONCLUSION: Real time PCR based validation of the selected DEGs showed that the qRT-PCR was consistent with the RNA-Seq results. This is the first transcriptomic study against bakanae disease of rice in Indian genotypes. Further, functional studies on identified genes and their utilization through different methodology will be helpful for the development of bakanae disease management strategies.
BACKGROUND: Fusarium fujikuroi causing bakanae is one of the most significant pathogens of rice and much responsible for yield losses thereby emerging as a major risk to food security. METHODS: In the present study transcriptomic analysis was conducted between two contrasting resistant (C101A51) and susceptible (Rasi) genotypes of rice with the combinations of C101A51 control (CC) vs. C101A51 inoculated (CI); Rasi control (RC) vs. Rasi inoculated (RI) and C101A51 inoculated (CI) vs. Rasi inoculated (RI). RESULTS: In CC vs. CI commonly expressed genes were 12,764. Out of them 567 (4%) were significantly upregulated and 1399 (9%) genes were downregulated. For the RC vs. RI 14, 333 (79%) genes were commonly expressed. For CI vs. RI 13,662 (72%) genes were commonly expressed. Genes related to cysteine proteinase inhibitor 10, disease resistance protein TAO1-like, oleosin 16 kDa-like, pathogenesis-related protein (PR1), (PR4), BTB/POZ and MATH domain-containing protein 5-like, alpha-amylase isozyme were upregulated in resistant genotype C101A51. Whereas, genes related to GDSL esterase/lipase, serine glyoxylate aminotransferase, CASP-like protein 2C1, WAT1-related protein, Cytoplasmic linker associated proteins, xyloglucan endotransglucosylase/hydrolase protein and β-D xylosidase 7 were upregulated in susceptible genotype Rasi. Gene ontology analysis showed functions related to defence response (GO:0006952), regulation of plant hypersensitive type response (GO:0010363), Potassium ion transmembrane activity (GO:0015079), chloroplast (GO:0009507), response to wounding (GO:0009611), xylan biosynthetic process (GO:0045492) were upregulated in resistant genotype C101A51 under inoculated conditions. CONCLUSION: Real time PCR based validation of the selected DEGs showed that the qRT-PCR was consistent with the RNA-Seq results. This is the first transcriptomic study against bakanae disease of rice in Indian genotypes. Further, functional studies on identified genes and their utilization through different methodology will be helpful for the development of bakanae disease management strategies.
Authors: Ednar G Wulff; Jens L Sørensen; Mette Lübeck; Kristian F Nielsen; Ulf Thrane; Jan Torp Journal: Environ Microbiol Date: 2009-11-25 Impact factor: 5.491
Authors: Bishnu M Bashyal; Kirti Rawat; Sapna Sharma; Deepika Kulshreshtha; S Gopala Krishnan; Ashok K Singh; Himanshu Dubey; Amolkumar U Solanke; T R Sharma; Rashmi Aggarwal Journal: Front Plant Sci Date: 2017-11-27 Impact factor: 5.753
Authors: Paolo Bagnaresi; Chiara Biselli; Luigi Orrù; Simona Urso; Laura Crispino; Pamela Abbruscato; Pietro Piffanelli; Elisabetta Lupotto; Luigi Cattivelli; Giampiero Valè Journal: PLoS One Date: 2012-12-12 Impact factor: 3.240
Authors: R Abdul Fiyaz; Ashutosh K Yadav; S Gopala Krishnan; Ranjith K Ellur; Bishnu M Bashyal; Nitasha Grover; Prolay K Bhowmick; M Nagarajan; K K Vinod; Nagendra K Singh; Kumble V Prabhu; Ashok K Singh Journal: Rice (N Y) Date: 2016-09-13 Impact factor: 4.783