Yongju Yang1,2, Yuqian Zhang3, Dongyu Min4, Heshan Yu5, Xuefeng Guan6. 1. The Ministry of National Education Key Lab for Traditional Chinese Medicine Visceral Manifestations Theory and Application, Liaoning University of Traditional Chinese Medicine, Shenyang, China. 2. Department of Orthopaedic Rehabilitation, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, China. 3. Academic Office, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, China. 4. Animal Experiment Center, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, China. 5. Laboratory Department, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, China. 6. Party and Government Office, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
Abstract
Background: At present, the progression mechanism of knee osteoarthritis (KOA) has not been fully elucidated, and there is a clinical need for late KOA-specific diagnostic markers to provide reference for preventive treatment. This study aimed to analyze the sequencing results of early- and late-stage KOA synovial tissue based on the key genes of late-stage KOA in combination with a machine learning algorithm. Methods: The whole transcriptome sequencing results of synovial tissue from KOA patients (GSE176223 and GSE32317) were downloaded from the gene expression omnibus (GEO) database. Thirty-nine early KOA synovial tissue samples and 31 late KOA synovial tissue samples were included in this study. The diagnostic criteria and baseline data balance of early and late KOA were referred to the data source literature, and the two groups of data had good baseline data balance. R software (V3.5.1) and R packages were used for screening and enrichment analysis of differentially expressed genes (DEGs). The key genes were screened by weighted correlation network analysis (WGCNA) and least absolute shrinkage and selection operator (LASSO) regression analysis. A receiver operating characteristic curve (ROC) curve was used to evaluate the diagnostic efficacy of key genes for advanced KOA. Results: A total of 211 DEGs related to knee arthritis were screened out. Compared with synovial tissue of early knee arthritis, 111 genes were upregulated and 100 genes were downregulated in the synovial tissue of late knee arthritis. Sixty-six key genes were screened out through WGCNA and 34 key genes were screened out in the LASSO analysis. The genes obtained by the two algorithms combined with three overlapping genes, namely interleukin- 6 (IL-6), C-X-C chemokine ligand 12 (CXCL12), and macrophage migration inhibitor factor (MIF). The areas under the ROC curves of CXCL12, IL-6, and MIF were 0.96, 0.944, and 0.961, respectively (P<0.001). Conclusions: IL-6, CXCL12, and MIF are the key pathogenic genes of KOA, which have good diagnostic efficacy for advanced KOA. 2022 Annals of Translational Medicine. All rights reserved.
Background: At present, the progression mechanism of knee osteoarthritis (KOA) has not been fully elucidated, and there is a clinical need for late KOA-specific diagnostic markers to provide reference for preventive treatment. This study aimed to analyze the sequencing results of early- and late-stage KOA synovial tissue based on the key genes of late-stage KOA in combination with a machine learning algorithm. Methods: The whole transcriptome sequencing results of synovial tissue from KOA patients (GSE176223 and GSE32317) were downloaded from the gene expression omnibus (GEO) database. Thirty-nine early KOA synovial tissue samples and 31 late KOA synovial tissue samples were included in this study. The diagnostic criteria and baseline data balance of early and late KOA were referred to the data source literature, and the two groups of data had good baseline data balance. R software (V3.5.1) and R packages were used for screening and enrichment analysis of differentially expressed genes (DEGs). The key genes were screened by weighted correlation network analysis (WGCNA) and least absolute shrinkage and selection operator (LASSO) regression analysis. A receiver operating characteristic curve (ROC) curve was used to evaluate the diagnostic efficacy of key genes for advanced KOA. Results: A total of 211 DEGs related to knee arthritis were screened out. Compared with synovial tissue of early knee arthritis, 111 genes were upregulated and 100 genes were downregulated in the synovial tissue of late knee arthritis. Sixty-six key genes were screened out through WGCNA and 34 key genes were screened out in the LASSO analysis. The genes obtained by the two algorithms combined with three overlapping genes, namely interleukin- 6 (IL-6), C-X-C chemokine ligand 12 (CXCL12), and macrophage migration inhibitor factor (MIF). The areas under the ROC curves of CXCL12, IL-6, and MIF were 0.96, 0.944, and 0.961, respectively (P<0.001). Conclusions: IL-6, CXCL12, and MIF are the key pathogenic genes of KOA, which have good diagnostic efficacy for advanced KOA. 2022 Annals of Translational Medicine. All rights reserved.
Authors: Marieke L A Landsmeer; Jos Runhaar; Marienke van Middelkoop; Edwin H G Oei; Dieuwke Schiphof; Patrick J E Bindels; Sita M A Bierma-Zeinstra Journal: J Am Board Fam Med Date: 2019 Jul-Aug Impact factor: 2.657
Authors: Qian Wang; Andrew L Rozelle; Christin M Lepus; Carla R Scanzello; Jason J Song; D Meegan Larsen; James F Crish; Gurkan Bebek; Susan Y Ritter; Tamsin M Lindstrom; Inyong Hwang; Heidi H Wong; Leonardo Punzi; Angelo Encarnacion; Mehrdad Shamloo; Stuart B Goodman; Tony Wyss-Coray; Steven R Goldring; Nirmal K Banda; Joshua M Thurman; Reuben Gobezie; Mary K Crow; V Michael Holers; David M Lee; William H Robinson Journal: Nat Med Date: 2011-11-06 Impact factor: 53.440
Authors: Monica Singh; Srishti Valecha; Rubanpal Khinda; Nitin Kumar; Surinderpal Singh; Pawan K Juneja; Taranpal Kaur; Mario Di Napoli; Jatinder S Minhas; Puneetpal Singh; Sarabjit Mastana Journal: Int J Environ Res Public Health Date: 2021-05-31 Impact factor: 3.390