Ultrastructural study of arterial wall repair after argon laser micro-anastomosis.

A carotid end-to-end anastomosis was performed in 50 Wistar rats by means of a Coherent 900 argon laser. The vessel sealing was obtained with laser shots (average 19) of 300 mW power and 5-second duration, the beam being focused in a spot of 150 micron diameter (1,700 W/cm2). From day 0 to day 120, 25 specimens underwent semithin and electron microscopic examinations. The results showed the immediate sealing effect of the argon laser owing to protein denaturation and collagen fusion of the media and adventitia 100 micron in width. Re-endothelialization of the anastomotic line began by day 3, while myofibroblast proliferation was observed in the media by day 10. By day 20 media scar was still occupied by numerous myofibroblasts and surrounded by abundant collagen and elastic fibres. At long term the endothelial repair was complete and the anastomotic strength was assured by medial collagenous network.