Young Kyu Kim1, JuKyung Lee2, Hyeon-Young Kim3, Sung-Hwan Kim3, Jeong Ho Hwang4,5, Han Na Suh6,7. 1. Animal Model Research Group, Korea Institute of Toxicology, 30 Baekhak1-gil, 56212, Jeongeup, Jellabuk-do, Republic of Korea. 2. Department of Medical IT Convergence, Kumoh National Institute of Technology, 39177, Gumi, Gyeongbuk, Korea. 3. Human Health Risk Assessment Center, Korea Institute of Toxicology, 30 Baekhak1-gil, 56212, Jeongeup, Jellabuk-do, Republic of Korea. 4. Animal Model Research Group, Korea Institute of Toxicology, 30 Baekhak1-gil, 56212, Jeongeup, Jellabuk-do, Republic of Korea. jeongho.hwang@kitox.re.kr. 5. Center for Companion Animal New Drug Development, Korea Institute of Toxicology, 30 Baekhak1-gil, 56212, Jeongeup, Jellabuk-do, Republic of Korea. jeongho.hwang@kitox.re.kr. 6. Animal Model Research Group, Korea Institute of Toxicology, 30 Baekhak1-gil, 56212, Jeongeup, Jellabuk-do, Republic of Korea. hanna.suh@kitox.re.kr. 7. Center for Companion Animal New Drug Development, Korea Institute of Toxicology, 30 Baekhak1-gil, 56212, Jeongeup, Jellabuk-do, Republic of Korea. hanna.suh@kitox.re.kr.
Abstract
BACKGROUND: Given its similar structure and immune response to the human skin, porcine is a good model for dermal studies. Here, we sensitized ovalbumin (Ova) on minipig back skin for 2-4 weeks to induce chronic atopic dermatitis (AD). RESULTS: Gross observation, serum cytokine level, epidermal thickness, and epidermal integrity did not change after 4 weeks of Ova induction compared with the control, indicating AD modeling failure. Only the neutrophils in the blood and macrophages in bronchoalveolar lavage fluid changed slightly until 3 or 2 weeks after Ova sensitization, respectively. The successful and failed Ova-induced AD minipig models only differ in age and body weight of the minipigs. The minipigs, 12 months old with a 30-kg median weight, had a two-fold thicker dermis than minipigs 8-10 months old, with an 18.97-kg median weight, resulting in impaired Ova permeability and immune response. CONCLUSION: Age and body weight are key factors that should be considered when developing an Ova-induced AD minipig model.
BACKGROUND: Given its similar structure and immune response to the human skin, porcine is a good model for dermal studies. Here, we sensitized ovalbumin (Ova) on minipig back skin for 2-4 weeks to induce chronic atopic dermatitis (AD). RESULTS: Gross observation, serum cytokine level, epidermal thickness, and epidermal integrity did not change after 4 weeks of Ova induction compared with the control, indicating AD modeling failure. Only the neutrophils in the blood and macrophages in bronchoalveolar lavage fluid changed slightly until 3 or 2 weeks after Ova sensitization, respectively. The successful and failed Ova-induced AD minipig models only differ in age and body weight of the minipigs. The minipigs, 12 months old with a 30-kg median weight, had a two-fold thicker dermis than minipigs 8-10 months old, with an 18.97-kg median weight, resulting in impaired Ova permeability and immune response. CONCLUSION: Age and body weight are key factors that should be considered when developing an Ova-induced AD minipig model.
Authors: Clarissa P Souza; Rod A W Rosychuk; Elena T Contreras; Jennifer R Schissler; Andrew C Simpson Journal: Vet Dermatol Date: 2018-08-23 Impact factor: 1.589
Authors: Kathryn O'Brien; Ayesha Bhatia; Fred Tsen; Mei Chen; Alex K Wong; David T Woodley; Wei Li Journal: PLoS One Date: 2014-12-02 Impact factor: 3.240