Successful treatment of disseminated nocardiosis diagnosed by metagenomic next-generation sequencing: A case report and review of literature.

BACKGROUND: Nocardia paucivorans is an infrequently found bacterium with the potential to cause severe infection, with a predilection for the central nervous system, both in immunocompromised and immunocompetent individuals. Rapid etiological diagnosis of nocardiosis can facilitate timely and rational antimicrobial treatment. Metagenomic next-generation sequencing (mNGS) can improve the rate and reduce the turnaround time for the detection of Nocardia. CASE
SUMMARY: A 49-year-old man was admitted to hospital with cough and hemoptysis. Imaging revealed pulmonary consolidation as well as multiple brain lesions. Nocardia asiatica and Nocardia beijingensis were rapidly detected by mNGS of bronchoalveolar lavage fluid (BALF) while bacterial culture of BALF and pathological biopsy of lung tissue were negative. In early stages, he was treated with trimethoprim-sulfamethoxazole (TMP-SMZ) and linezolid by individual dose adjustment based on serum concentrations and the adverse effects of thrombocytopenia and leukopenia. The treatment was then replaced by TMP-SMZ and ceftriaxone or minocycline. He was treated with 8 mo of parenteral and/or oral antibiotics, and obvious clinical improvement was achieved with resolution of pulmonary and brain lesions on repeat imaging.
CONCLUSION: mNGS provided fast and precise pathogen detection of Nocardia. In disseminated nocardiosis, linezolid is an important alternative that can give a better outcome with the monitoring of linezolid serum concentrations and platelet count. ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.

Core Tip: Early detection of Nocardia paucivorans can optimize antibiotic management, shorten hospital stays and improve survival rates. We report rapid detection of N. paucivorans by metagenomic next-generation sequencing (mNGS) of BALF. The patient was treated with trimethoprim-sulfamethoxazole (TMP-SMZ) and linezolid by individual dose adjustment based on serum concentration and thrombocytopenia and leukopenia, then replaced by TMP-SMZ and ceftriaxone or minocycline. This case suggests that mNGS is a convenient and efficient technique for detecting Nocardia, especially suitable for rare, novel and atypical etiologies of complicated infectious diseases. Linezolid may be an important alternative in disseminated nocardiosis.

INTRODUCTION

Nocardia paucivorans is a Gram-positive aerobic bacterium of the order Actinomycetales and has become an increasingly important opportunistic pathogen, mostly in immunocompromised individuals[1]. It causes a wide spectrum of illness, including pneumonia, cutaneous or subcutaneous infections, brain or other solid organ abscesses, thus resulting in high mortality[2]. Early detection of N. paucivorans can optimize antibiotic management, shorten hospital stays, improve survival rates and reduce medical costs. Delaying diagnosis may impede targeted therapies and lead to poor prognosis. However, the definitive diagnosis of nocardiosis still depends on the isolation and identification of organisms from the infected site, which may take days to weeks and the positive rates by these methods are very low. As these methods cannot meet the current demand for rapid and accurate diagnosis, metagenome next- generation sequencing (mNGS) has become available in clinical practice[3]. mNGS also known as shotgun deep-sequencing, is a high-throughput sequencing approach with more rapid and accurate diagnostic advantages than traditional methods, especially in culture-negative samples[4,5]. mNGS can simultaneously detect and identify pathogens by extracting only a small amount of DNA or RNA from samples. mNGS can theoretically detect all pathogens in clinical samples, especially for rare, novel, and atypical etiologies of complex infectious diseases[6]. Here, we describe a case of disseminated nocardiosis in a 49-year-old man diagnosed by mNGS of bronchoalveolar lavage fluid (BALF) rather than bacterial culture of BALF and pathological biopsy of lung tissue. He was successfully treated with parenteral or oral antibiotics for 8 mo, with evident clinical improvement and resolution of pulmonary and brain lesions on repeat imaging.

CASE PRESENTATION

Chief complaints

A 49-year-old Chinese man had symptoms of cough and hemoptysis for 2 mo.

History of present illness

A 49-year-old Chinese man presented to the hospital with 2 mo of cough and hemoptysis, but without fever or chest pain. Daily blood loss was estimated to be 20-30mL. During a visit to a different hospital on March 10, 2020, chest computed tomography (CT) demonstrated a mass and atelectasis in the right upper lobe (Figure 1A). BALF was obtained by brochoscopy; however, the bacterial culture of BALF was negative. The patient was diagnosed with pulmonary infection and treated with oral cefixime capsules (0.2g every 12h) and azithromycin (500 mg/d) for 1 wk. However, there was no improvement and the patient was in a poor general status. He had dyspnea when walking hastily or going up stairs and had chest pain. Chest CT on April 23, 2020 showed aggravation of the original lesion, especially in the right upper lobe (Figure 1B). He was admitted to our respiratory department for further evaluation on April 25, 2020.

Figure 1

Computed tomography of right lung. A: The first scan demonstrated a mass and atelectasis in the right upper lobe on March 20, 2020; B: Chest Computed Tomography (CT) showed aggravation of the original lesion on April 23, 2020; C: Positron emission tomography-CT showed that the mass lesions in the posterior segment of the right upper lobe had significantly increased glucose metabolism inhomogeneously on April 27, 2020; D: Primary lesion of the right upper lobe reduced on May 23, 2020; E-J: Repeated pulmonary CT showed primary lesion size of the right upper lobe continued to decrease on different times, involving June 22, 2020 (E), July 29, 2020 (F), September 8, 2020 (G), November 20, 2020 (H), January 7, 2021 (I) and March 25, 2021 (J). CT: Computed tomography.

History of past illness

The patient had no previous medical history, and no history of drug allergy.

Personal and family history

The patient worked as a welder and lived with his wife and children. He had no history of cigarette smoking or alcohol abuse. There was no family history to note.

Physical examination

On admission, the patient was afebrile and vital signs were normal. Breath sounds were decreased in the right lung, and neurological examination was unremarkable.

Laboratory examinations

The oxygenation index in blood gas analysis was 265 mmHg (Table 1). Leukocytosis was 14.36 × 109/L, C-reactive protein was 117.50 mg/L, and procalcitonin was 0.126 ng/mL, and liver and kidney functions were normal. GeneXpert MTB/RIF, quantitative immunoglobulins, 1-3-β-D-glucan, galactomannan (GM), cryptococcal and deep sputum bacteriological culture antigen were all negative.

Table 1

Efficacy before and after treatment

	April 25, 2020	May 23, 2020	June 16, 2020
Cough	2	1	0
Hemoptysis	20-30 mL	0	0
Dyspnea index	2	1	0
Supplemental O2 in L/min	4 L/min	2 L/min	0
Oxygenation index	265 mmHg	388 mmHg	412 mmHg

Cough index: 0: NO cough; 1: Occasionally have a cough; 2: Frequent cough, mild impact on daily life; 3: Frequent cough, serious impact on daily life. Hemoptysis volume: Small amount: < 100 mL/24 h; Moderate amount: 100-500 mL/24 h; Massive amount: > 500 mL/24 h or > 100 mL at one time. Dyspnea index: 0: Asymptomatic while climbing stairs; 1: Symptomatic while climbing stairs; 2: Symptomatic after walking 100 m on flat ground; 3: Symptomatic with the least effort (e.g., talking, getting dressed); 4: Symptomatic in bed, at rest. Oxygenation index: partial blood oxygen divided by oxygen concentration.

Imaging examinations

Positron emission tomography (PET)-CT was ordered on the suspicion of lung cancer. PET-CT showed that most of the mass lesions in the posterior segment of the right upper lobe had significantly increased glucose metabolism inhomogeneously with peripheral exudative changes, which were suspicious for infection on April 27, 2020 (Figure 1C).

Further diagnostic work-up

The patient was inhaled oxygen 4 L/min and treated empirically with simultaneous cefoperazone-tazobactam and teicoplanin for 7 d, but he continued to experience cough and hemoptysis with elevated C-reactive protein (109.92 mg/L) and procalcitonin (0.189 ng/mL). Meropenem and teicoplanin were then administered. In addition, bronchoscopy was performed to obtain specimens including BALF and lung tissue. Transbronchial biopsy of soft tissue of the right upper lung showed chronic inflammation with acute activity and fibrous tissue hyperplasia with focal necrosis. GeneXpert MTB/RIF, GM (1-3)-β-D-Glucan, fungi and acid-fast bacillus stains, and BALF culture for bacteria were negative. However, mNGS analysis of BALF detected N. asiatica and N. beijingensis. The patient developed a new symptom of headache at the same time. Cranial enhanced magnetic resonance imaging (MRI) was ordered on the suspicion of intracranial Nocardia infection and it showed multiple lesions in the left occipital lobe, right cerebellar hemisphere and left frontal lobe on May 13, 2020 (Figure 2A). Cerebrospinal fluid (CSF) obtained by lumbar puncture identified no white or red blood cells, glucose 3.42 mmol/L and protein 0.54 g/L. The bacterial culture, cryptococcal antigen, virus DNA and exfoliative cell examination of CSF were all negative, as was NGS analysis of CSF (Figure 3).

Figure 2

Brain magnetic resonance imaging findings. A: Cranial magnetic resonance imaging (MRI) showed multiple lesions in the left occipital lobe, right cerebellar hemisphere and left frontal lobe on May 13, 2020; B: Cranial MRI showed a decrease of the original lesion on June 15, 2020; C: Primary lesions of the brain maintained shrinkage on July 31, 2020; D: Primary lesions of the brain almost completely disappeared on September 10, 2020. MRI: Magnetic resonance imaging.

Figure 3

Chronic inflammation with acute activity and fibrous hyperplasia, with focal necrosis in the right upper lobe. A: A large number of chronic inflammatory cells with a small amount of neutrophil infiltration in hyperplastic fibrous connective tissue (Original magnification: 100 ×; scale bar: 100 μm); B: There are collagen hyperplasia and no obvious alveolar epithelial cells in some areas (Original magnification: 200 ×; scale bar: 100 μm); C and D: In addition, there are phagocytic cells that engulf carbonic acid in some areas, inflammatory cell infiltration and capillary endothelial cells in the interstitium (C: 200 ×; scale bar: 100 μm; D: 400 ×; scale bar: 100 μm).

FINAL DIAGNOSIS

Disseminated nocardiosis affecting the CNS and lungs.

TREATMENT

The patient was immediately treated with oral TMP-SMZ 0.96 g every 8 h and intravenous linezolid 600 mg every 12 h from May 9, 2020. After 2 wk of combined antibiotic therapy, his condition improved and pulmonary CT showed an obvious reduction in consolidation in both lung fields on May 23, 2020 (Figure 1D). The oxygenation index in blood gas analysis was 388mmHg (Table 1). Platelet count showed thrombocytopenia (171 × 109 /L 109 /L vs normal 233 × 109 /L). After 3 wk, because of the higher dose of linezolid in the plasma (9.36 mg/L) and reduction of hemamebas (3.34 × 109/L) and platelets (122 × 109/L), we adjusted the dose of intravenous linezolid (600 mg/d) and oral TMP-SMZ (0.96 g every 12 h) on May 31, 2020 (Table 2). The patient’s blood count recovered gradually, and at the same time his clinical conditions continued to improve. After 5 wk and improvement of his brain lesions on repeat MRI (Figure 2B), as the dose of linezolid in plasma was still high (6.38 mg/L), the treatment was replaced by ceftriaxone (2 g/d) on June 16, 2020 (Table 2). After 1 wk of treatment with ceftriaxone (2 g/d) and TMP-SMZ (0.96 g every 12 h), we shifted the antibiotic therapy to oral minocyline (100 mg every 12 h) and TMP-SMZ (0.96 g every 12 h) for 6.5 mo (Table 2). He completed nearly 8 mo of antibiotic therapy, with resolution of his pulmonary and brain lesions (Figure 1E-J), and complete cure was achieved (Figure 2C and D).

Table 2

Therapeutic strategy and the laboratory examinations

	April 9, 2020	May 30, 2020	May 31, 2020	June 16, 2020	June 23, 2020
Treatment	TMP-SMZ 0.96 g q8 h + linezolid 0.6 g q12 h		TMP-SMZ 0.96 g q12 h + linezolid 0.6 g qd	TMP-SMZ 0.96 g q12 h + ceftriaxone 2 g/d	TMP-SMZ 0.96 g q12 h and minocyline 100 mg q12 h
WBC (109/L)	4.03		3.34	4.07	3.91
PLT (109 /L)	379		130	219	185
The dose of linezolid (mg/L)	No		9.36	6.38	No

TMP-SMZ: Trimethoprim-sulfamethoxazole; WBC: White Blood Cell; PLT: Platelet.

OUTCOME AND FOLLOW-UP

He completed nearly 8 mo of antibiotic therapy, with resolution of his pulmonary and brain lesions (Figure 1E-J), and complete cure was achieved (Figure 2C and D).

DISCUSSION

Disseminated nocardiosis may affect many organs, especially the lungs, CNS, skin and even the pericardium. Among disseminated infections, 44% of patients had CNS involvement, highlighting the neurotrophic features of Nocardia spp. CNS manifestations of Nocardia infection can include isolated or multifocal brain abscesses and meningitis[7-9]. Consistent with the previous reports, our patient presented with disseminated disease spreading from a primary pulmonary infection to the central nervous system. He had a neurological symptom of headache and MRI revealed multiple lesions in the left frontal lobe, occipital lobe and right cerebellar hemisphere. Although bacterial culture and NGS analysis of CSF were both negative, his headache disappeared and MRI showed that the craniocerebral lesions were significantly reduced or even disappeared after antibiotic therapy, which confirmed that he had cerebral nocardiosis.

A definitive diagnosis of nocardiosis requires the isolation and identification of the organism from a clinical specimen. The traditional detection methods in specimens for microbial culture, histopathology, or smear microscopy, have low yield and are time-consuming; therefore, NGS has emerged[3]. NGS can detect unknown pathogens and a variety of mixed infectious pathogens by one-stop precision sequencing, therefore simplifying the detection process, improving pathogen detection sensitivity, and shortening detection time[4,5]. In this case, N. asiatica and N. beijingensis were rapidly detected by the mNGS of BALF, while the bacterial culture of BALF and the pathological biopsy of lung tissue were negative, contributing to prompt and accurate antibiotic treatment, and obvious clinical improvement was observed with resolution of pulmonary and brain lesions on repeat imaging. As reported, NGS can enable in-depth identification and classification of pathogens and their abilities to resist current treatment methods. In addition, the analysis of drug genes and virulence factors has an incomparable advantage over traditional detection methods[10,11]. However, as mNGS usually detects more than one pathogen in one test, clinicians need to comprehensively evaluate the clinical status and sequencing results of the patients to distinguish pathogens, symbiotic flora and pollutants. Although this method could detect the common resistant genes, it was unable to provide direct formation about antibiotic sensitivity. Furthermore, the high cost and low accessibility of NGS still limit its application in clinical practice. In the future, optimization of sample acquisition, sample preparation and bioinformatics analysis will be essential to the application of mNGS in clinical diagnosis. We hope that in the near future, this technology will benefit more patients.

Treatment of CNS nocardiosis is largely based upon expert opinion. Popular treatment strategies include empirical combination intravenous therapy with agents such as imipenem, TMP-SMZ, linezolid and amikacin for several weeks followed by antibiogram-guided oral therapy[12,13]. Consistent with these strategies, our patient initiated treatment with parenteral and oral antibiotics for 6 wk and had definite clinical improvement, followed by switching to oral minocyline and TMP-SMZ for 6.5 mo. He completed nearly 8 mo of antibiotic therapy, with resolution of his brain and pulmonary lesions and achieved complete cure. Furthermore, this successful case confirmed that in some cases of disseminated nocardiosis, linezolid may be an important alternative that can give a better outcome. However, it should be emphasized that the risk of adverse effects might be increased if linezolid was combined with other antibiotics. Postmarketing studies have reported higher rates of linezolid-associated thrombocytopenia, ranging from 15% to 50%[14,15]. The higher dose of linezolid in the plasma and prolonged duration of therapy have been suggested as more important risk factors[16-19]. Consistent with these findings, our study identified longer duration of therapy as a risk factor. The first thrombocytopenic platelet count occurred after 14 d of linezolid therapy. The severity of thrombocytopenia increased with the duration of treatment. Similarly, in this case, thrombocytopenia was closely related to the higher dose of linezolid in the plasma. The combined treatment of linezolid and TMP-SMZ may aggravate the myelosuppressive effects of reversible neutropenia and thrombocytopenia. Selection of appropriate antibiotics given at the optimal time is hence crucial for effective therapy. Although NGS can detect the presence of drug resistant genes in pathogens, it may not be consistent with clinical drug susceptibility results. Furthermore, there are still many drug resistant genes that have not yet been discovered and false negative results may exist.

A PubMed/MEDLINE search of literature found a total of 17 cases within a decade except for the references already cited in the article (Table 3)[20-36]. For patients with underlying conditions (87.5% in immunocompromised individuals), affected organs (lung involvement is most common, followed by the brain), and positive results (mostly from culture and histopathology, but not the NGS), TMP-SMZ is the most commonly used and effective drug.

Table 3

Cases of pulmonary nocardiosis reported in recent 10 yr

Ref.	Predisposing or underlying condition	Affected organ (s)	Positive specimen (s)	Anti-biotic therapy	Outcome
Komiya et al[20], 2013	Hypersensitivity pneumonitis	Lung	sputum	Meropenem, TMP-SMZ	Improved
Yasar et al[21], 2014	No	Lung	Lung tissue	TMP-SMZ	Improved
Cooper et al[22], 2014	Smoking and alcohol consumption	Lung +Brain	Tissue of lung and brain	Vancomycin, Ceftriaxone, Amphotericin B, TMP-SMZ	Dead
Yagi et al[23], 2014	Mycobacterium avium	Lung	Sputum	TMP-SMZ	Improved
Ibrahim et al[24], 2016	Hematopoietic stem cell transplant	Lung +Brain	Lung tissue	TMP-SMZ	Improved
Mendonca et al[25], 2016	Multiple Myeloma	Lung	BALF	TMP-SMZ, Meropenem, Tigecycline	Improved
Jayaschandran et al[26], 2016	Idiopathic CD4 T-lymphocytopenia	Lung	BALF	Meropenemz, TMP-SMZ	Improved
Khadka et al[27], 2017	Renal transplant, Pulmonary tuber-culosis	Lung+Brain+ Celiac lymph nodes	BALF	TMP-SMZ	Improved
Kobayashi et al[28], 2018	Olfactory neuroblastoma, Ectopic adrenocorticotropic hormone	Lung	BALF	TMP-SMZ	Improved
Khaliq et al[29], 2019	Cladophialophora bantiana Cerebral Phaeohyphomycosis	Lung	Lung tissue	TMP-SMZ, Meropenem, Voriconazole, Liposomal amphotericin	Died
Miyaoka et al[30], 2019	Smoking	Lung	BALF	Meropenem, TMP-SMZ	Improved
Kato et al[31], 2019	Radiation pneumonitis treated with prednisolone [PSL (50 mg/d)]	Lung	The PCR of 16S rRNA gene of the BALF	TMP-SMZ, Doripenem	Improved
Deterding et al[32], 2020	heart transplant recipient requiring dialysis	lung	BALF	Amikacin, Imipenem	Improved
Campoli et al[33], 2020	Liver transplant, Cutaneous infection by Alternaria alternata	Lung	BALF	TMP-SMZ, Linezolid, Ceftriaxone	Improved
Meena et al[34], 2020	Pulmonary tuberculosis and COPD	Lung	BALF	TMP-SMZ	Improved
Wu et al[35], 2021	Pulmonary alveolar proteinosis	Lung	Lung tissue	TMP-SMZ	Improved
Atemnkeng et al[36], 2021	COVID-19 Pneumonia	Lung +Brain	BALF	Meropenem, TMP-SMZ, Linezolid	Improved

TMP-SMZ: Trimethoprim-sulfamethoxazole; BALF: Bronchoalveolar lavage fluid; PCR: Polymerase Chain Reaction; COPD: Chronic obstructive pulmonary disease.

CONCLUSION

This case illustrates that Nocardia may cause severe disseminated infection in immunocompetent patients, which often involves the CNS and lungs. mNGS can establish a more rapid and accurate diagnosis than traditional methods. It is a convenient and efficient technique for the detection of Nocardia, and is especially suitable for rare, novel and atypical etiologies of complicated infectious diseases. This successfully treated case confirmed that, in some patients with disseminated nocardiosis, TMP-SMZ is still the first choice of treatment, but linezolid may be an important alternative that can give a better outcome with the monitoring of linezolid serum concentrations and platelet count. Notable risk factors for linezolid-induced thrombocytopenia included high daily weight-based dose and prolonged duration of therapy (some patients experience thrombocytopenia within 14 d of treatment). Clinicians should monitor patients for linezolid-induced thrombocytopenia throughout therapy.