Liqin Jiang1, Xinyu Liu1, Lei Zhou1,2, Joanna M Fianza Busoy1, Myo Thu Khine1, Yee Shan Dan1, Mengyuan Ke1, Noel A Brennan3, Karen J V Catbagan1, Leopold Schmetterer1,4, Veluchamy A Barathi1,2, Quan V Hoang1,2,5. 1. Singapore Eye Research Institute, Singapore National Eye Centre, Duke-NUS Medical School, Singapore. 2. Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. 3. Johnson & Johnson Vision, Jacksonville, Florida, United States. 4. School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore. 5. Department of Ophthalmology, Columbia University, New York, New York, United States.
Abstract
Purpose: To identify choroidal characteristics associated with susceptibility to development of naturally occurring and experimentally induced myopia. Methods: We compared choroidal properties between pigmented and albino guinea pig (GP) strains. Biometry, cycloplegic refractive error (RE), and eye wall sublayer thickness were measured from 171 GPs at postnatal day (P)6, 14, and 28. Forty-three P14 GPs underwent two-week monocular form-deprivation myopia (FDM). En face images of choroidal vasculature were obtained with a customized swept-source optical coherence tomography. Multivariate regression analyses were performed, with P28 RE as the outcome and P14 choroidal thickness (ChT) as the main predictor variable. Proteomic analysis was performed on choroidal tissue from P14 albino and pigmented GPs. Results: At P14, RE was correlated with thickness of the choroid (β = 0.06), sclera (β = 0.12), and retina (β = 0.27; all P < 0.001). P14 ChT was correlated with P28 RE both with (β = 0.06, P = 0.0007) and without FDM (β = 0.05, P = 0.008). Multivariate regression analysis, taking into account FDM (versus physiological growth) and strain, revealed that for every 10-µm greater ChT at P14, P28 RE was 0.50D more positive (P = 0.005, n = 70). En face images of choroidal sublayers showed that albino choroids were relatively underdeveloped, with frequent avascular regions. Consistent with this finding, proteomic analysis suggested abnormalities of the nitric oxide system in the albino GP choroid. Conclusions: Current results are consistent with the notion that greater ChT could protect from or delay the onset of myopia, while lower ChT is associated with greater susceptibility to myopia development. The underlying mechanism could be related to dysfunction of the choroidal vascular system.
Purpose: To identify choroidal characteristics associated with susceptibility to development of naturally occurring and experimentally induced myopia. Methods: We compared choroidal properties between pigmented and albino guinea pig (GP) strains. Biometry, cycloplegic refractive error (RE), and eye wall sublayer thickness were measured from 171 GPs at postnatal day (P)6, 14, and 28. Forty-three P14 GPs underwent two-week monocular form-deprivation myopia (FDM). En face images of choroidal vasculature were obtained with a customized swept-source optical coherence tomography. Multivariate regression analyses were performed, with P28 RE as the outcome and P14 choroidal thickness (ChT) as the main predictor variable. Proteomic analysis was performed on choroidal tissue from P14 albino and pigmented GPs. Results: At P14, RE was correlated with thickness of the choroid (β = 0.06), sclera (β = 0.12), and retina (β = 0.27; all P < 0.001). P14 ChT was correlated with P28 RE both with (β = 0.06, P = 0.0007) and without FDM (β = 0.05, P = 0.008). Multivariate regression analysis, taking into account FDM (versus physiological growth) and strain, revealed that for every 10-µm greater ChT at P14, P28 RE was 0.50D more positive (P = 0.005, n = 70). En face images of choroidal sublayers showed that albino choroids were relatively underdeveloped, with frequent avascular regions. Consistent with this finding, proteomic analysis suggested abnormalities of the nitric oxide system in the albino GP choroid. Conclusions: Current results are consistent with the notion that greater ChT could protect from or delay the onset of myopia, while lower ChT is associated with greater susceptibility to myopia development. The underlying mechanism could be related to dysfunction of the choroidal vascular system.
Authors: Rebecca J Sardell; Muneeswar G Nittala; Larry D Adams; Reneé A Laux; Jessica N Cooke Bailey; Denise Fuzzell; Sarada Fuzzell; Lori Reinhart-Mercer; Laura J Caywood; Violet Horst; Tine Mackay; Debbie Dana; SriniVas R Sadda; William K Scott; Dwight Stambolian; Jonathan L Haines; Margaret A Pericak-Vance Journal: Ophthalmology Date: 2016-10-19 Impact factor: 12.079
Authors: Jamin S Brown; D Ian Flitcroft; Gui-shuang Ying; Ellie L Francis; Gregor F Schmid; Graham E Quinn; Richard A Stone Journal: Invest Ophthalmol Vis Sci Date: 2008-08-21 Impact factor: 4.799