Wolf's Isotopic Response After Herpes Zoster Infection in Chronic Lichen Sclerosus-Like Graft versus Host Disease: Case Report and Literature Review.

Wolf's isotopic response (WIR) refers to the occurrence of a new skin disease at the exact site of an unrelated skin disease that had previously healed, often subsequent to virus infection. Secondary cutaneous diseases that are frequently observed in WIR include granulomatous reactions, dysimmune reactions, malignancies, and infections. However, secondary chronic graft-versus-host disease (GVHD) is rare. We describe a patient with lichen sclerosus-like GVHD who developed lichen planus-like GVHD lesions secondary to herpes zoster infection.

Case Report

A 36-year-old female with acute lymphoblastic leukemia (B-cell type) underwent an allogeneic hematopoietic stem cell transplantation (allo-HSCT) five years ago from a human leukocyte antigen (HLA) fully matched related donor in Peking University Institute of Hematology, Peking University People’s Hospital. The conditioning regimen with hydroxyurea, cytarabine, busulfan, cyclophosphamide and semustine was used. After HSCT, graft-versus-host disease (GVHD) prophylaxis was performed with cyclosporine and prednisone for 3 months. And then, it was changed to dasatinib for consolidation. The patient did not develop acute GVHD. Eighteen months after HSCT, the patient developed oral leukoplakia and scattered gray-white patches on the bilateral ribs. Dasatinib was discontinued temporarily and was resumed in another center after a diagnosis of “chronic GVHD”. The lesions on the trunk gradually resolved. Fifty-two months after HSCT, the patient felt persistent neuralgia on the right side of the head and face, followed by erythema and clustered blisters in a band-like distribution. She was diagnosed with herpes zoster in other hospitals and received antiviral treatment with valacyclovir. The cutaneous lesions and neuralgia improved in two weeks, although postherpetic pigmentation was observed. Fifty-four months after HSCT, the patient was conscious of the appearance of dark red flat polygonal papules with an overlying network of white lines at the site of postherpetic pigmentation. She then went to our department for further examination and treatment. Physical examination: Poorly defined gray-white sclerotic plaques were observed on the neck and trunk with a slightly cigarette-paper epidermal atrophy. Some sclerotic plaques were surrounded by purple-red patches (Figure 1). A band of dark red, polygonal, flat papules with well-defined boundaries were observed on the right forehead. Hyperpigmentation was visible on the right eyelid (Figure 2). Diagnoses include chronic cutaneous GVHD, lichen sclerosus type on the trunk and lichen planus type on the head and face (Wolf’s isotopic response after herpes zoster infection). The lesions did not spread and were resolved with 5mg of oral prednisone and 0.05% halometasone monohydrate cream.

Figure 1

Scattered light brown, grayish-white patches were observed on the trunk and neck, symmetrically distributed, with poorly defined borders, and the central part of the site may show parchment-like atrophy. (A-D) Trunk; (E) Neck.

Figure 2

A band-like distribution of lichen planus-like lesions was observed on the right forehead, and the right eyelid was heavily pigmented.

Discussion

In 1995, Wolf et al defined the isotopic response as the occurrence of a new skin disease at the exact site of an unrelated skin disease that had previously healed. The interval between the primary trauma and the onset of WIR is variable, ranging from weeks to years, although most cases occur during the period of active disease.1 Wolf et al have recently expanded the definition of healed skin diseases that trigger an isotopic response, which now includes “scars, pigment changes, color changes or various other minimal changes by the first disease”.2 Infection with varicella-zoster virus (VZV) or herpes simplex virus (HSV) is the most common pre-disposing skin disorder for an isotopic response.3 Of all the isotopic responses following a VZV/HSV infection, the most frequent secondary cutaneous disease is granulomatous reaction, followed by malignancies, leukaemic or lymphomatous infiltrations, dysimmune reactions, infections, comedonic-microcystic reactions, and keloid.3 Clinically, the cutaneous manifestations also exhibited various appearances. Psoriasis, bullous pemphigoid, eosinophilic dermatitis, and dermatitis neglecta have also been reported to manifest as isotopic responses.4 However, cutaneous GVHD is rarely reported as the manifestation of WIR.

The pathogenesis of WIR after herpes zoster remains unclear; potential mechanisms include viral-induced, vascular or immunologic disorder, as well as a neural-centered hypothesis (which may be the most widely accepted hypothesis). VZV infection causes changes in antigenicity of keratinocytes that may act as targets for donor effector cells.5 Other hypotheses suggest that viral infection may increase the expression of human leukocyte antigen (HLA)-II antigens and adhesion molecules on keratinocytes, or that antibodies against viral antigens may cross-react with host HLA molecules.5 VZV may also modify the normal host immune response by reversing the ratio of CD4+/CD8+ T lymphocyte and altering the activity of cytotoxic T-lymphocytes and natural-killer cells.5 The density of epidermal nerve endings is reduced at sites previously infected with VZV compared to normal skin. Nerve injury causes neuropeptide release, which leads to immune dysfunction and alters immune control in the affected dermatome. Neuroimmune destabilization in the VZV-infected site may be mediated by the continuous influence of viral DNA.4

The definitive pathophysiologic mechanisms of chronic GVHD are complex. After injury, keratinocytes may express pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs), leading to the release of inflammatory factors from recipient antigen-presenting cells (APC) and the subsequent T-cell activation and proliferation. Chemotherapy, pre-transplantation conditioning, acute GVHD and other processes can damage the recipient’s thymus, resulting in defective recessive selection and the production of autoreactive T cells. The unbalanced proportions of Th1, Th2, and Th17 cells, as well as decreased amounts of the regulatory T cells, regulatory B cells and natural killer cells, contribute to B cell activation and autoantibody production through cytotoxicity, various inflammatory factors and the antigen presentation by APC. Meanwhile, macrophages are activated to generate extracellular matrix, INF-γ, TNF-α, perforin and granzymes, leading to the destruction of recipient tissues, autoantibody secretion after B cell activation and the recruitment of other effector cells. These actions lead to organ damage and tissue fibrosis, resulting in the development of chronic GVHD.6 Accordingly, epithelial cell damage and immune abnormalities after herpes zoster are the most possible causes of secondary chronic GVHD.

As with many other inflammatory dermatoses, immune dysregulation was considered the predominant mechanism for the development of lichen planus (LP). Activated T cells, principally cytotoxic CD8+ T cells, launch an immune attack on basal keratinocytes with the help of CD4+ helper T cells secreting Th1 cytokines, resulting in LP-like lesions.7 LP has also been associated with numerous viral infections, including hepatitis C virus, hepatitis B virus, and VZV.7,8 In addition, genetic and environmental factors may also play roles in pathogenesis of LP.7 The development of lichen sclerosus (LS) lesions may be associated with excess T lymphocyte clones, with increased expression of autoimmune-related inflammatory cytokines (interleukins IL-1, IL-12, IL-2, IL-5, IL-10, tumor necrosis factor-α, interferon-γ).9 Upregulation of microRNA-155 levels reduced the suppression of CD4+ T cells by regulatory T cells and possibly promoted the proliferation of fibroblasts, leading to the epidermal sclerosis.9 The previously unrecognized existence of resident memory CD8+ T cells may also contribute to the pathogenesis of lichenoid reactions in postherpetic WIR lesions.10 Thus, the ratio of CD4+ and CD8+ T lymphocytes may be associated with the type of skin lesions. Chronic GVHD caused by the WIR after herpes zoster infection manifests as lichenoid-like lesions, which may indicate that CD8+ T lymphocytes are mainly up-regulated after VZV infection, but the specific mechanisms require further research.

WIR after herpes zoster infection manifested by cutaneous GVHD is rarely reported. We reviewed a total of 9 cases with this diagnosis, according to the current report (lichenoid type in 6 cases, sclerotic type in 2 cases and unknown in 1 case). Specific clinical features, the presence or absence of acute GVHD, and the interval of the herpes zoster infection and the setting of chronic GVHD are shown in Table 1. In our case, the isotopic response manifested as a typical lichen planus-like lesion, a diagnostic manifestation of chronic GVHD, therefore no histopathological examination was performed. When cutaneous lesions appear as erythematous, papules, and blisters, the differential diagnosis of persistent herpes zoster should be considered. Persistent herpes zoster often occurs under long-term immunosuppression.11 Serum VZV-IgM, IgG detection, histopathological examination and skin tissue herpes zoster virus polymerase chain reaction (PCR) detection can be performed when the identification is difficult.

Table 1

Reported Cases of Chronic Cutaneous GVHD After Herpes Zoster Infection as a Result of Wolf’s Isotopic Response

Source	Number of Patients	Sex	Age/Years	Primary Disease	Transplant
Our case	1	F	36	ALL	PBHSC, ARD
Palacios 201512	1	F	62	MDS	BM, ARD
Mehra 201213	1	M	69	MDS	BM, ADU
Martires 201114	1	M	60	BCL	PBHSC, ADU
Raymond 201115	1	M	47	PV	PBHSC, ARD
Kroth 201116	1	M	34	AML	PBHSC, ADU
Sanli 200317	2	F	23; 47	CML	BM, ARD
Lacour 199918	1	M	4	ALL	BM, AURD
Baselga 199619	1	F	16	MLD	BM, ARD
Source	Number of Patients	Acute GVHD	Site	Type of Chronic GVHD	Interval/Months
Our case	1	No	Head	Lichenoid	2
Palacios 201512	1	No	Trunk	Lichenoid	1
Mehra 201213	1	No	Trunk	Lichenoid	8
Martires 201114	1	/	Trunk	Sclerotic	38
Raymond 201115	1	Yes	Upper limb	Sclerotic	2
Kroth 201116	1	/	Trunk	/	12
Sanli 200317	2	No; Yes	Trunk; Neck	Lichenoid	3
Lacour 199918	1	Yes	Trunk and lower limb	Lichenoid	6
Baselga 199619	1	Yes	Neck	Lichenoid	1

Abbreviations: ALL, acute lymphoblastic leukemia; MDS, myelodysplastic syndrome; BCL, B-cell lymphoma; PV, polycythemia vera; AML, acute myeloid leukemia; CML, chronic myeloid leukemia; MLD, metachromatic leukodystrophy; PBHSC, peripheral blood hematopoietic stem cell; BM, bone marrow; ADU, allogeneic, donor unknown; ARD, allogeneic related donor; AURD, allogeneic unrelated donor; GVHD, graft-versus-host disease; Interval, time from the resolution of the herpes zoster virus infection and the onset of chronic GVHD.

Conclusion

We describe a patient with chronic GVHD with an LS-like primary lesion who developed a typical WIR at the site of herpes zoster infection, presenting as LP-like GVHD. After literature review, we found that the ratio of CD4+ and CD8+ T lymphocytes may be related to the type of skin lesions. Chronic GVHD with WIR after herpes zoster infection mostly manifests as lichenoid lesions, which may indicate an upregulated proportion of CD8+ T lymphocytes.