Literature DB >> 36223267

Characterizing diversity among human oral stem-like cancer cells using flow cytometry.

Subhashis Ghosh1, Paromita Mitra1, Uday Saha1, Kavya Vipparthi1, Sandeep Singh2.   

Abstract

We have developed the protocol of flow cytometry for characterizing diversity among oral stem-like cancer cells (SLCCs) using CD44, CD24, and aldehyde dehydrogenase (ALDH) in oral tumors. We are also reporting the protocol for tumor-derived explant cultures to develop oral cancer cell lines and enriching these diverse hybrid states of cancer cells in 3D spheroids from established cell lines. For complete details on the use and execution of this protocol, please refer to Vipparthi et al. (2022) and Vipparthi et al. (2021).
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Cancer; Cell biology; Cell culture; Cell differentiation; Cell separation/fractionation; Flow cytometry/Mass cytometry; Single cell; Stem cells

Year:  2022        PMID： 36223267      PMCID： PMC9576741          DOI： 10.1016/j.xpro.2022.101763

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  7 in total

1.  Two novel cell culture models of buccal mucosal oral cancer from patients with no risk-habits of tobacco smoking or chewing.

Authors:  Kavya Vipparthi; Ankit Kumar Patel; Subhashis Ghosh; Subrata Das; Chitrarpita Das; Koyeli Das; Anwesha Sarkar; Venu Thatikonda; Biswajoy Pal; Arun Sasi Kumaran Nair Remani; Neeraj Arora; Mayur Parihar; Maleppillil Vavachan Vijayakumar; Manoj Kumar Bhat; Ramanamurthy Boppana; Samsiddhi Bhattacharjee; Nidhan Kumar Biswas; Pattatheyil Arun; Rajeev Sharan; Sandeep Singh
Journal:  Oral Oncol       Date:  2020-12-30       Impact factor: 5.337

2.  Oral cancer: risk factors and molecular pathogenesis.

Authors:  Hari Ram; Jayanta Sarkar; Hemant Kumar; Rituraj Konwar; M L B Bhatt; Shadab Mohammad
Journal:  J Maxillofac Oral Surg       Date:  2011-04-22

3.  Intratumor genetic heterogeneity in squamous cell carcinoma of the oral cavity.

Authors:  Dan P Zandberg; Luke J Tallon; Sushma Nagaraj; Lisa K Sadzewicz; Yuji Zhang; Maxwell B Strome; Xuechu E Zhao; Kranthi Vavikolanu; Xiaoyu Zhang; John C Papadimitriou; Fleesie A Hubbard; Søren M Bentzen; Scott E Strome; Claire M Fraser
Journal:  Head Neck       Date:  2019-03-14       Impact factor: 3.147

Review 4.  Management of gingivobuccal complex cancer.

Authors:  Sanjeev Misra; Arun Chaturvedi; N C Misra
Journal:  Ann R Coll Surg Engl       Date:  2008-08-12       Impact factor: 1.891

5.  Dissociation of human oral mucosal tissue for single-cell applications.

Authors:  Teresa Greenwell-Wild; Drake Winslow Williams; Niki Maria Moutsopoulos
Journal:  STAR Protoc       Date:  2021-10-22

6.  Emergence of hybrid states of stem-like cancer cells correlates with poor prognosis in oral cancer.

Authors:  Kavya Vipparthi; Kishore Hari; Priyanka Chakraborty; Subhashis Ghosh; Ankit Kumar Patel; Arnab Ghosh; Nidhan Kumar Biswas; Rajeev Sharan; Pattatheyil Arun; Mohit Kumar Jolly; Sandeep Singh
Journal:  iScience       Date:  2022-04-27

Review 7.  Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Authors:  Caleb Jensen; Yong Teng
Journal:  Front Mol Biosci       Date:  2020-03-06
  7 in total

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