Literature DB >> 3621570

Optimized liquid-chromatographic method for fluorometric determination of urinary delta-aminolevulinic acid in workers exposed to lead.

K Tomokuni, M Ichiba, Y Hirai, T Hasegawa.   

Abstract

We developed a fluorometric method for determining delta-aminolevulinic acid (ALA) in urine of lead workers. A high-performance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 micrograms/L (15 pmol of ALA in the 100-microL sample). The working linear range of urinary ALA concentration was 0.1 to 100 mg/L. In 25 lead-exposed workers, ALA values by the present method for urine correlated well with those obtained by a conventional colorimetric method (r = 0.996). The advantage of the present method for micro-determination of urinary ALA is its high sensitivity.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3621570

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  8 in total

1.  Tyrosinaemia type I with normal levels of plasma tyrosine.

Authors:  I T de Almeida; P P Leandro; M F Silva; C Silveira; A da Silva; J S de Sousa; M Duran
Journal:  J Inherit Metab Dis       Date:  1990       Impact factor: 4.982

2.  Motor neuropathy in porphobilinogen deaminase-deficient mice imitates the peripheral neuropathy of human acute porphyria.

Authors:  R L Lindberg; R Martini; M Baumgartner; B Erne; J Borg; J Zielasek; K Ricker; A Steck; K V Toyka; U A Meyer
Journal:  J Clin Invest       Date:  1999-04       Impact factor: 14.808

3.  Effect of lead exposure on some biological indices related to porphyrin metabolism and the activity of erythrocyte pyrimidine 5'-nucleotidase in the mice.

Authors:  K Tomokuni; M Ichiba; Y Hirai
Journal:  Arch Toxicol       Date:  1989       Impact factor: 5.153

4.  A method for determining δ-aminolevulinic acid synthase activity in homogenized cells and tissues.

Authors:  Hector A Bergonia; Michael R Franklin; James P Kushner; John D Phillips
Journal:  Clin Biochem       Date:  2015-05-08       Impact factor: 3.281

5.  Comparison between males and females with respect to the porphyrin metabolic disorders found in workers occupationally exposed to lead.

Authors:  H Oishi; H Nomiyama; K Nomiyama; K Tomokuni
Journal:  Int Arch Occup Environ Health       Date:  1996       Impact factor: 3.015

6.  Comparison between the fluorometric HPLC method and the conventional method for determining urinary delta-aminolevulinic acid and coproporphyrin as indices of lead exposure.

Authors:  K Tomokuni; M Ichiba; Y Hirai; K Sugimoto; T Yoshida; M Hirata
Journal:  Int Arch Occup Environ Health       Date:  1988       Impact factor: 3.015

7.  The effect of aluminum chloride on some steps of heme biosynthesis in rats after oral exposure.

Authors:  J Chmielnicka; M Nasiadek; E Lewandowska-Zyndul
Journal:  Biol Trace Elem Res       Date:  1994-02       Impact factor: 3.738

8.  In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles.

Authors:  Lei Shi; Xiuli Wang; Feng Zhao; Hansen Luan; Qingfeng Tu; Zheng Huang; Hao Wang; Hongwei Wang
Journal:  Int J Nanomedicine       Date:  2013-07-24
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.