The tragedy of smoking, alcohol, and multiple substance use during pregnancy.

BACKGROUND: Antenatal substance use is a significant public health concern in South Africa (SA). Information on smoking, drinking and drug use during pregnancy was collected prospectively for the Safe Passage Study of the PASS (Prenatal Alcohol in Sudden infant death syndrome and Stillbirth) Network.
OBJECTIVES: Data from 4 926 pregnant women in a community near Tygerberg Academic Hospital, Cape Town, were examined to determine whether associations between different substance use groups and postnatal infant outcomes at birth and 1 year were significant.
METHODS: Gestational age (GA) was determined by earliest ultrasound. Maternal data were collected at enrolment or first antenatal visit. Substance use data were obtained at up to four occasions. Birthweight data were derived from medical records, and birthweight z-scores (BWZs) were specifically calculated using INTERGROWTH-21st study data. Statistical analyses were done with Statistica version 13.  Results. Women who used more substances enrolled later, were younger, and had smaller mid-upper arm circumferences (MUACs), less education and lower monthly income than women who used no substances (control group). Infants born to women who used more substances had lower GA at delivery, birthweight and BWZ than infants from the control group. At 1 year, infants born to women who used more substances had a lower weight, shorter length and smaller head circumference. Education was positively associated with all infant outcomes at birth and 1 year. MUAC was positively associated with infant BWZ, and weight and length at 1 year. Income was negatively associated with BWZ, but positively associated with all 1-year outcomes.
CONCLUSION: Substance use during pregnancy affects infant outcomes at birth and 1 year of age. The addictive properties of substance use make cessation difficult, so prevention strategies should be implemented long before pregnancy. Higher maternal education, associated with better infant outcomes at birth and 1 year and acting as a countermeasure to substance use, is of paramount importance.

Substance use during pregnancy is on the increase worldwide[ and is a significant public health concern.[ In South Africa (SA), use of multiple substances during pregnancy is common. In a survey of 5 232 pregnant women visiting midwife obstetric units in Cape Town, it was found that 36.9% used alcohol and drugs, 34.9% alcohol only, and 1.6% drugs only.[ Also in Cape Town, a substudy of the Safe Passage Study (SPS) of the PASS (Prenatal Alcohol in Sudden infant death syndrome and Stillbirth) Network, on the value of maternal serum alpha-fetoprotein measurements, found that 61% of pregnant women smoked, 55% drank alcohol, and 9% and 5% used marijuana and methamphetamine, respectively. [ Methamphetamine use in pregnancy is associated with poorer neonatal outcomes, especially decreased birthweight, head circumference and body length.[ The effects of marijuana use during pregnancy are less clear, with reports ranging from no adverse effect with regard to the likelihood of prematurity or low birthweight (LBW)[ to a reduction in birthweight, length and head circumference[ and an increase in preterm births and growth restriction (GR).[

The association of marijuana use with poor perinatal outcome seems to be attributable to concomitant use of tobacco and other confounding factors.[ Perinatal outcome is particularly susceptible to socioeconomic conditions affecting lifestyle choices and behaviour.[ Low socioeconomic status and lower educational attainment increase the risk of smoking during pregnancy significantly.[ Smoking is not only associated with complications such as preterm birth, GR and stillbirth,[ but has long-term maternal implications such as lung cancer, cardiovascular and chronic respiratory disease, oral diseases and strokes, and long-term infant implications such as respiratory problems (e.g. childhood asthma), infections, obesity, cleft lip/palate, and neurodevelopmental and behavioural problems.[

Interestingly, only the effect of cocaine on birthweight remained significant after adjusting for confounding variables.[ It is important to note that very few pregnant women use methamphetamine or marijuana on their own; most of them also use nicotine or alcohol, or both. In a study of 12 069 pregnant women, it was found that 45% of marijuana users also smoked.[ The same applied to users of methamphetamine, of whom 78.6%, 42.9% and 39.3% used tobacco, alcohol, and marijuana, respectively.[

Of all three health-compromising behaviours, smoking, alcohol consumption and recreational drug use, cigarette smoking has been most studied and strongly implicated in reduced fetal growth.[ Our previous finding that significantly more pregnant smokers than pregnant non-smokers engaged in heavy alcohol consumption[ is supported by Okah et al.[ They found that pregnant smokers were seven times more likely than non-smokers to use alcohol and/or drugs, and that the rate of heavy smoking and moderate/heavy drinking increased with the number of health-compromising behaviours. Infants antenatally exposed to both alcohol and cigarettes had a substantially higher risk of sudden infant death syndrome compared with those who were unexposed, or exposed to alcohol or cigarettes alone.[

As the information on smoking, drinking and drug use for the SPS was collected prospectively, this database was ideal to examine the interactions of substance use during pregnancy on infant outcome.[

Methods

The SPS was designed to investigate the role of prenatal alcohol exposure in the outcome of 12 000 pregnancies in SA (Cape Town) and the USA (Northern Plains). Women recruited included those with low- and high-risk pregnancies, with a wide range of exposures to alcohol, nicotine, marijuana and methamphetamine. [ The present study was limited to the SA arm of the SPS, where participants were recruited at a community health centre close to Tygerberg Academic Hospital (TAH), Cape Town. Participants were enrolled between August 2007 and January 2015 and infants were followed up until the end of August 2016. Gestational age (GA) was determined by earliest ultrasound before the second antenatal visit. Depending on the GA at enrolment, women had up to three further antenatal visits at TAH, at 20 – 24, 28 – 32 and 34 – 38 weeks. The revised Timeline Followback method was used at up to four occasions to obtain detailed information on drinking, cigarette smoking, and the use of marijuana, amphetamines and other substances during pregnancy.[ Anaemia was based on laboratory results of a haemoglobin value <11 g/dL during pregnancy and obtained from medical chart abstraction (MCA). Demographic and anthropometric information was obtained at enrolment or the first antenatal visit. Maternal weight was measured twice, using a regularly calibrated high-quality scale. For the mid-upper arm circumference (MUAC), the midpoint of the upper arm was first determined and then the circumference measured twice. If any two measurements differed by >1 kg (weight) or 2 mm (MUAC), a third measurement was taken and the mean of the closest two measurements used.

A pregnancy loss or fetal death before 20 weeks, according to the US definition for the SPS, was defined as a miscarriage, whereas a non-live birth at ≥20 weeks was regarded as a stillbirth.[ Terminations of pregnancies after 20 weeks were done for medical reasons. Death of a liveborn infant before the age of 1 year was defined as an infant death. A social worker, employed for the SPS, was available to all women for counselling if necessary or requested.

Newborns were weighed immediately after birth and the information was entered in the maternal chart, from where it was obtained by MCA after delivery. The GA at delivery, obtained from the electronic data capturing (EDC) system, together with fetal sex was used to determine birthweight z-scores (BWZs) and centiles specifically for us upon request, from the international standards of the INTERGROWTH-21st study (available for GAs from 168 to 299 days, excluding twins).[

The infants were seen at 1 year of age and the assessment date was adjusted for prematurity, e.g. an infant born 10 weeks (70 days) early had a required 1-year age of birth date + 365 + 70 days to birth date + 365 + 70 + 30 days at 1-year assessment. At the beginning of our study, infants born at term were required to have an age of 365 − 30 to 365 + 30 days at their 1-year examination, but this was soon changed to between birth date + 365 days and birth date + 365 + 30 days. Infants were weighed (1YW), and their length (1YL) and head circumference (1YHC) were measured by trained research workers according to a specific protocol. For weighing the infants, a Charder digital baby scale was used (Charder Electronic Co. Ltd, Taiwan). The child, dressed in a clean, dry diaper, with a vest during winter, was weighed to the nearest 0.1 kg. The process was repeated, and if the measurements differed by >0.2 kg, a third measurement was taken. A Seca 416 infantometer (Seca Deutchland, Germany) was used to measure the length to the nearest millimetre. The full procedure was repeated for a second measurement and if it differed by >2 mm, a third measurement was taken. A flexible tape measure was used to measure the head circumference to the nearest millimetre while the child was sitting on the mother’s lap or lying down. The tape measure was placed over the occipital protuberance at the back of the head and around to just over the supraorbital ridge and the forehead in front. The procedure was repeated, and done a third time if the first two measurements differed by >2 mm. All the measurements were entered on a specific case report form, and later on the EDC system.

To examine the effects of various combinations of exposure to nicotine, alcohol, marijuana (Mar) and methamphetamine (Met), 11 different combinations were used, namely no exposure (Control), NoDrugsDrink, NoDrugsSmoke, NoDrugsDrinkSmoke, MarSmoke, MarDrink, MarDrinkSmoke, MetDrink, MetSmoke, MetDrinkSmoke, and All (used all four substances). Since only 12 and 2 participants used only marijuana or only methamphetamine, respectively, separate groups for these drugs were not developed and they were excluded from the cohort. Outcome variables studied were BWZ, 1YW, 1YL and 1YHC. Since we, and others, have shown that MUAC, maternal education and household income play important roles in newborn and 1-year outcomes, these were used as confounders.[

Statistical analyses were performed using the Statistica data analysis software system, version 13 (TIBCO Software Inc., USA). Descriptive statistics were used to describe continuous variables, which were compared between groups with analysis of variance (ANOVA). Bonferroni or least significant difference multiple comparisons identified significant differences between the means in the ANOVA. Non-parametric tests such as the Mann-Whitney U-test or the Kruskal-Wallis test compared differences between groups where responses were not normally distributed. Two-way ANOVAs were used to compare the influence of two factors on continuous response variables. The maximum likelihood χ2 test determined significance in categorical data and was used to compare the substance use groups with the Control group. Spearman correlations measured correlations between ordinal/continuous response variables. A p-value <0.05 indicated statistical significance. The three prespecified confounding variables were used in multiple regression analyses with 11 groups of smoking, drinking, marijuana and methamphetamine combinations for each of the four outcome variables to determine their association and the underlying effect of substance use.

Ethics approval for the study was obtained from the Health Research Ethics Committee of Stellenbosch University (ref. nos N06/10/210 and S19/07/119), as well as from the Western Cape Department of Health. Participants were able to withdraw at any time during the study.

Results

The full cohort consisted of 4 926 pregnant women, of whom 877 (17.8%) used no drugs, cigarettes or alcohol (Control), 825 (16.7%) used no drugs but drank (NoDrugsDrink), 862 (17.5%) used no drugs but smoked (NoDrugsSmoke), 1 801 (36.6%) used cigarettes and alcohol (NoDrugsDrinkSmoke), 64 (1.3%) used marijuana and cigarettes (MarSmoke), 27 (0.5%) used methamphetamine and cigarettes (MetSmoke), 20 (0.4%) used marijuana and alcohol (MarDrink), 11 (0.2%) used methamphetamine and alcohol (MetDrink), 274 (5.6%) used marijuana, alcohol and cigarettes (MarDrinkSmoke), 88 (1.8%) used methamphetamine, alcohol and cigarettes (MetDrinkSmoke), and 77 (1.6%) used all four substances (All). This equated to 65% of women who smoked, 63% of women who drank, 9% of women who used marijuana and 4% of women who used methamphetamine. Excluded from the cohort were twin pregnancies, withdrawals, participants lost to follow-up, women who used marijuana or methamphetamine alone or had missing substance use data, and multiple enrolments. Only the first enrolment of a participant was included in this cohort. Preterm birth (<37 weeks) and very preterm birth (<32 weeks) occurred in 598 (12.1%) and 85 (1.7%) women, respectively. Of the total cohort (4 926 women), 65 (1.3%) were HIV positive, 1 979 (40.2%) were anaemic, 8 (0.2%) had a miscarriage, 7 (0.1%) had a termination of pregnancy, 657 (13.3%) had LBW infants who weighed <2 500 g, 840 (17.1%) had small-for-gestational-age (SGA) infants who fell below the 10th birthweight centile, 44 (0.9%) had a stillbirth, and 45 (0.9%) had an infant death.

Information on the biometric measurements and socioeconomic conditions is provided in Table 1.

Table 1.

Basic descriptive statistics of all participants

Variables	Valid N	Mean	Median	Minimum	Maximum	Lower quartile	Upper quartile	SD
Gestational age at enrolment (days)	4 926	142	141	38*	276	105	177	49
Maternal age (years)	4 926	24.4	23	16	45	20	28	6.0
Maternal arm circumference (mm)	4 838	276	267	175	535	241	303	46
Maternal body mass index (kg/m2)	4 787	25.6	24.2	13.7	55.9	21.2	28.9	5.8
Gravidity	4 916	2.1	2	1	10	1	3	1.3
Education (years)	4 919	10.1	10	2	13	9	12	1.7
Household income (ZAR/month)	3 500	886	750	45	6 000	500	1 200	607
Gestational age at delivery (days)	4 926	272	275	61[†]	313	267	282	18
Birthweight (g)	4 862	3 016	3 030	190	5 740	2 700	3 380	574
Birthweight z-score	4 847	−0.34	−0.37	−6.34	4.12	−1.04	0.33	1.03
Infant age at 1 year (days)	4 500	372	369	330[‡]	475[§]	366	377	17
Infant weight at 1 year (kg)	4 490	9.4	9.3	5.3	16.9	8.5	10.3	1.4
Infant length at 1 year (cm)	4 408	73.7	73.7	60.7	88.0	71.8	75.6	3.0
Infant head circumference at 1 year (cm)	4 479	46.1	46.0	41.1	54.7[¶]	45.1	47.0	1.5

SD = standard deviation.

Single case that deviated from required 6 weeks, but permission obtained to keep included.

Miscarriages included.

Initial time window minimum that was corrected later.

Time window maximum adjusted for prematurity.

The only excessively large value, not removed.

Table 2 summarises the maternal biometric measurements and socioeconomic conditions that were compared for the different substance use groups. Women in the Control group enrolled the earliest for antenatal care, had the largest MUAC and BMI, and also earned the highest mean income per month. Women in the MetSmoke group enrolled the latest, had the highest gravidity without being the oldest women, had the smallest mean MUAC, had the lowest average monthly income, and had the joint lowest education together with the MarSmoke and All groups. Women in the MarDrink group had the joint lowest gravidity and the highest education. Women in the MetDrink group were the oldest and had the joint highest gravidity. Women in the MarDrinkSmoke group were the youngest, had the joint lowest gravidity, had the lowest BMI, and were significantly the most anaemic.

Table 2.

Biometric measurements and socioeconomic conditions compared in different substance use groups

			Substance use group
Variables	F p-value	Measure	Control (n=877)	NoDrugsDrink (n=825)	NoDrugsSmoke (n=862)	NoDrugsDrinkSmoke (n=1 801)	MarSmoke (n=64)	MetSmoke (n=27)	MarDrink (n=20)	Met Drink (n=11)	MarDrinkSmoke (n=274)	MetDrinkSmoke (n=88)	All (n=77)
Gestational age at enrolment (days)	<0.01*	Letters	d	cd	cd	c	bcd	a	bcd	abc	cd	b	b
		Mean	137[†]	142	142	142	145	184[‡]	151	170	141	154	157
		SD	49	48	50	49	52	47	49	42	45	53	46
Maternal age (years)	<0.01*	Letters	a	bc	ab	cd	de	abcd	bcde	abcd	e	bcd	bcd
		Mean	25.7	24.6	24.9	24.1	22.1	24.7	21.3	26.6[‡]	20.4[†]	23.6	23.0
		SD	6.2	5.8	6.2	5.8	5.8	4.7	4.8	4.7	4.4	4.4	4.7
Maternal arm circumference (mm)	<0.01*	Letters	a	ab	bc	c	d	d	abcd	abcd	d	cd	cd
		Mean	286[‡]	283	276	273	253	250[†]	265	271	253	265	260
		SD	51	49	47	43	32	26	39	31	37	39	42
Body mass index (kg/m2)	<0.01*	Letters	a	a	b	bc	d	bcd	abcd	abcd	d	cd	cd
		Mean	26.8[‡]	26.6	25.6	25.3	23.0	23.1	24.2	25.0	22.7[†]	23.8	23.4
		SD	6.2	6.1	5.8	5.5	4.0	3.8	4.6	3.1	4.1	4.3	5.0
Gravidity	<0.01*	Letters	abce	df	ab	cdef	cdefg	acd	befg	abcdefg	g	abcdef	abcdef
		Mean	2.2	1.9	2.4	2.1	1.8	2.8[†]	1.5[†]	2.8[‡]	1.5*[†]	2.2	2.1
		SD	1.2	1.2	1.4	1.2	1.1	1.4	1.1	1.6	0.9	1.4	1.2
Education (years)	<0.01*	Letters	b	a	c	c	d	d	ab	abcd	d	d	d
		Mean	10.5	10.7	9.9	9.9	9.1[†]	9.1[†]	10.8[‡]	9.9	9.4	9.4	9.1[†]
		SD	1.7	1.6	1.7	1.7	1.6	2.0	1.4	1.7	1.5	1.5	1.6
Household income (ZAR)	<0.01*	Letters	a	ab	bc	c	cd	abcd	abcd	abcd	d	cd	d
		Mean	997[‡]	987	880	844	639	566[†]	902	699	636	720	573
		SD	667	597	601	586	514	483	525	296	460	515	539
Anaemia with haemoglobin <11 g/dL	Compared with Control	N	345	319	350	725	26	12	12	5	127	30	28
		%	39.3	38.7	40.6	40.3	40.6	44.4	60.0	45.5	46.4	34.1	36.4
		χ2 p-value	-	0.776	0.590	0.649	0.839	0.593	0.062	0.680	0.039*	0.336	0.608

Mar = marijuana; Met = methamphetamine; Letters = significance lettering. If the significance lettering between two groups have common letters (e.g. b and bcd), the groups do not differ significantly; SD = standard deviation.

Significant at p<0.05 (F or χ2).

Smallest mean value.

Largest mean value.

Infant outcomes at birth and 1 year were compared in the different substance use groups and are summarised in Table 3. Infants from the Control group were heaviest at birth, had the largest BWZ, and were joint heaviest at 1 year. Infants from the NoDrugsSmoke group were significantly more premature, with more LBW and GR (SGA), and had more deaths compared with the Control group. Infants from the NoDrugsDrink group had the highest GA at birth and were joint heaviest at 1 year, whereas infants from the MarDrink group had the largest mean length and head circumference at 1 year. Infants from the MetDrink group had the lowest mean GA (<37 weeks) and more were premature; they had the lowest birthweight, and more were stillborn. Those alive at 1 year also had the lowest mean weight, lowest mean length and lowest mean head circumference, despite their adjusted age at 1 year. The MetSmoke group had the highest significant rate of infant deaths. Infants from the MarDrinkSmoke group had the lowest BWZ, and compared with the Control group had the highest proportion who had LBW and were SGA.

Table 3.

Infant outcome at birth and 1 year compared in different substance use groups

			Substance use group
Variables	F p-value	Continuous data measure	Control (n=877)	NoDrugsDrink (n=825)	NoDrugsSmoke (n=862)	NoDrugsDrinkSmoke (n=1 801)	MarSmoke (n=64)	MetSmoke (n=27)	MarDrink (n=20)	MetDrink (n=11)	MarDrinkSmoke (n=274)	MetDrinkSmoke (n=88)	All (n=77)
Gestational age at delivery	<0.01*	Letters	b	a	cd	bc	cd	de	abcd	e	bcd	cd	d
		Mean	273	275[‡]	271	272	268	265	269	255[†]	271	269	268
		SD	20	15	18	18	19	12	29	23	16	11	15
Birthweight	<0.01*	Letters	a	a	b	b	cd	abcd	abc	d	cd	bcd	cd
		Mean	3 131[‡]	3 111	2 994	2 976	2 818	2 913	3 029	2 564[†]	2 851	2 932	2 812
		SD	585	536	596	567	536	453	503	772	566	463	531
Birthweight z-score	<0.01*	Letters	a	b	b	c	cd	abc	abcd	abcd	d	abc	cd
		Mean	−0.14[‡]	−0.24	−0.32	−0.43	−0.64	−0.20	−0.44	−0.21	−0.66[†]	−0.32	−0.61
		SD	1.1	1.0	1.0	1.0	0.9	1.0	1.0	1.0	0.9	0.9	0.9
Infant age at 1 year	0.02*	Letters	c	c	ab	abc	b	b	abc	abc	ab	abc	ac
		Mean	371	371	373	372	376	379	375	382[‡]	373	373	369*[†]
		SD	16	15	18	17	17	16	11	35	19	16	17
Infant weight at 1 year	<0.01*	Letters	ab	a	cd	c	bcde	e	abcde	e	e	de	e
		Mean	9.6[‡]	9.6[‡]	9.4	9.4	9.2	8.6	9.3	8.3[†]	9.2	9.1	8.9
		SD	1.4	1.4	1.3	1.4	1.2	1.1	1.3	0.9	1.3	1.5	1.2
Infant length at 1 year	<0.01*	Letters	a	a	b	b	bc	c	ab	abc	c	c	c
		Mean	74.2	74.2	73.7	73.6	73.2	72.3	74.6[‡]	72.1[†]	73.0	72.7	72.5
		SD	2.9	3.0	3.0	3.0	2.6	2.6	2.8	2.4	3.1	2.7	3.3
Infant head circumference at year	<0.01*	Letters	ab	b	ac	c	cd	cd	abc	d	cd	abc	cd
		Mean	46.2	46.2	46.0	46.0	45.7	45.6	46.4[‡]	44.9[†]	45.9	46.0	45.7
		SD	1.5	1.5	1.4	1.5	1.3	1.4	1.5	1.4	1.5	1.4	1.5
			Substance use group
Variables	χ2 p-value	Categorical data measure	Control (n=877)	NoDrugsDrink (n=825)	NoDrugsSmoke (n=862)	NoDrugsDrinkSmoke (n=1 801)	MarSmoke (n=64)	MetSmoke (n=27)	MarDrink (n=20)	MetDrink (n=11)	MarDrinkSmoke (n=274)	MetDrinkSmoke (n=88)	All (n=77)
Preterm birth <37 weeks	Compared with Control	N	93	68	129	224	10	4	3	5	34	15	13
		%	10.6	8.2	15.0	12.4	15.6	14.8	15.0	45.5[§]	12.4	17.0	16.9
		χ2 p-value	-	0.096	0.006*	0.168	0.214	0.486	0.530	<0.001*	0.405	0.068	0.093
Very preterm birth <32 weeks	Compared with Control	N	16	8	18	32	2	0	1	1	6	0	1
		%	1.8	1.0	2.1	1.8	3.1	0.0	5.0	9.1	2.2	0.0	1.3
		χ2 p-value	-	0.135	0.691	0.931	0.463	0.479	0.303	0.080	0.700	0.201	0.738
Low birthweight <2 500 g	Compared with Control	N	87	75	123	267	10	4	4	3	56	13	15
		%	9.9	9.1	14.3	14.8	15.6	14.8	20.0	27.3	20.4[§]	14.8	19.5
		χ2 p-value	-	0.560	0.005*	<0.001*	0.147	0.405	0.140	0.058	<0.001*	0.154	0.009*
Growth-restricted infant <10th centile	Compared with Control	N	116	106	146	350	13	3	4	1	69	13	19
		%	13.2	12.8	16.9	19.4	20.3	11.1	20.0	9.1	25.2[§]	14.8	24.7
		χ2 p-value	-	0.817	0.031*	<0.001*	0.112	0.749	0.379	0.687	<0.001*	0.685	0.006*
Miscarriage <20 weeks	Compared with Control	N	3	0	1	3	0	0	1	0	0	0	0
		%	0.3	0.0	0.1	0.2	0.0	0.0	5.0	0.0	0.0	0.0	0.0
		χ2 p-value	-	0.093	0.325	0.367	0.639	0.761	0.002*	0.846	0.332	0.583	0.607
Termination of pregnancy	Compared with Control	N	2	2	0	3	0	0	0	0	0	0	0
		%	0.2	0.2	0.0	0.2	0.0	0.0	0.0	0.0	0.0	0.0	0.0
		χ2 p-value	-	0.951	0.161	0.729	0.702	0.804	0.831	0.874	0.429	0.654	0.675
Stillbirth	Compared with Control	N	8	4	6	17	1	0	0	1	2	2	3
		%	0.9	0.5	0.7	0.9	1.6	0.0	0.0	9.1[§]	0.7	2.3	3.9
		χ2 p-value	-	0.292	0.614	0.936	0.606	0.618	0.668	0.007*	0.777	0.230	0.019*
Infant death	Compared with Control	N	3	1	14	20	0	2	0	0	2	2	1
		%	0.3	0.1	1.6	1.1	0.0	7.4[§]	0.0	0.0	0.7	2.3	1.3
		χ2 p-value	-	0.347	0.007*	0.043*	0.639	<0.001*	0.793	0.846	0.394	0.016*	0.213

Mar = marijuana; Met = methamphetamine; Letters = significance lettering. If the significance lettering between two groups have common letters (e.g. b and bcd), the groups do not differ significantly; SD = standard deviation.

Significant at p<0.05 (F or χ2).

Smallest mean value.

Largest mean value.

Highest significant rate.

The maternal measures of GA at enrolment, age, MUAC and education as found in 11 substance use groups are presented in Figs 1, 2, 3 and 4, respectively. The birth outcomes of GA at delivery, birthweight and BWZ in the different substance use groups are shown in Figs 5, 6 and 7, respectively. The 1-year visit outcomes of 1YW, 1YL and 1YHC in the different substance use groups are shown in Figs 8, 9 and 10, respectively.

Fig. 1.

Gestational age at enrolment compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Gestational age at enrolment differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Fig. 2.

Maternal age compared among different substance use groups (weighted means). Vertical bars denote 0.95 confidence intervals. Maternal age differed significantly between any two groups when there was no overlap of letters above the vertical bars. (Mar = marijuana; Met = methamphetamine.)

Fig. 3.

Maternal mid-upper arm circumference compared among different substance use groups (weighted means). Vertical bars denote 0.95 confidence intervals. Maternal arm circumference differed significantly between any two groups when there was no overlap of letters above the vertical bars. (Mar = marijuana; Met = methamphetamine.)

Fig. 4.

Maternal education compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Maternal education differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Fig. 5.

Gestational age at delivery compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Gestational age at delivery differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Fig. 6.

Birthweight compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Birthweight differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Fig. 7.

Birthweight z-score compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Birthweight z-score differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Fig. 8.

Infant 1-year weight compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Infant 1-year weight differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Fig. 9.

Infant 1-year length compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Infant 1-year length differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Fig. 10.

Infant 1-year head circumference compared among different substance use groups (least-square means). Vertical bars denote 0.95 confidence intervals. Infant 1-year head circumference differed significantly between any two groups when there was no overlap of letters above the vertical bars. (ANOVA = analysis of variance; Mar = marijuana; Met = methamphetamine.)

Table 4 summarises the multiple regression results for BWZ. There was a positive association between BWZ and MUAC for all the groups that did not use drugs. The strongest associations were in the Control and the NoDrugsDrink groups, which also had the largest MUACs. BWZ was positively associated with education in only two groups, NoDrugsDrink and MarDrink, and these two groups also had the highest education. BWZ was negatively associated with income in the MetDrink group only. In this group, a higher income was associated with a lower BWZ, whereas a lower income was associated with a higher BWZ.

Table 4.

Multiple regression summary for birth outcome variable birthweight z-score

		MUAC		Education		Income
Substance use group	n	bz	p-value	bz	p-value	bz	p-value
Control	646	0.31	<0.001*	−0.00	0.929	0.04	0.272
NoDrugsDrink	596	0.26	<0.001*	0.10	0.021*	−0.06	0.151
NoDrugsSmoke	590	0.18	<0.001*	0.01	0.898	0.02	0.664
NoDrugsDrinkSmoke	1 208	0.16	<0.001*	0.05	0.072	0.04	0.152
MarSmoke	41	0.29	0.090	−0.07	0.683	0.15	0.392
MetSmoke	16	0.45	0.244	−0.30	0.354	−0.28	0.445
MarDrink	11	−0.17	0.614	1.04	0.046*	−0.66	0.214
MetDrink	7	−0.05	0.865	0.62	0.058	−0.98	0.043*
MarDrinkSmoke	154	0.10	0.221	−0.02	0.788	0.04	0.683
MetDrinkSmoke	56	0.18	0.182	0.09	0.505	0.16	0.239
All	50	0.16	0.291	0.03	0.856	−0.04	0.830

MUAC = mid-upper arm circumference; bz = standardised regression coefficient; Mar = marijuana; Met = methamphetamine.

Significant at p<0.05.

Table 5 summarises the multiple regression results for 1YW. There was a positive association between infant weight at 1 year and MUAC for all the groups that did not use drugs, apart from the All group. Mothers in the All group had 4th-lowest MUAC, that was associated with the 3rd-lowest weight at 1 year. There was also a positive association between 1-year weight of the infant and education of the mother for the Control, NoDrugsDrink, NoDrugsSmoke, NoDrugsDrinkSmoke, MarDrinkSmoke and MetDrinkSmoke groups. There was a positive association between 1-year weight of the infant and income of the mother in the NoDrugsDrinkSmoke group. These mothers earned the 5th-highest income and had infants with the 3rd-largest weights at 1 year.

Table 5.

Multiple regression summary for outcome variable infant weight at 1 year

		MUAC		Education		Income
Substance use group	n	bz	p-value	bz	p-value	bz	p-value
Control	608	0.14	<0.001*	0.12	0.003*	0.02	0.668
NoDrugsDrink	550	0.13	0.003*	0.15	0.001*	−0.00	0.916
NoDrugsSmoke	538	0.11	0.013*	0.09	0.047*	0.09	0.060
NoDrugsDrinkSmoke	1 109	0.14	<0.001*	0.12	<0.001*	0.11	<0.001*
MarSmoke	36	0.21	0.236	0.13	0.489	0.23	0.225
MetSmoke	14	0.56	0.178	−0.34	0.313	0.15	0.726
MarDrink	10	−0.40	0.347	0.73	0.184	−0.47	0.426
MetDrink	6	0.42	0.692	0.15	0.837	0.66	0.564
MarDrinkSmoke	145	0.08	0.319	0.21	0.015*	0.12	0.180
MetDrinkSmoke	48	0.08	0.573	0.42	0.003*	0.09	0.515
All	43	0.35	0.029*	−0.07	0.707	0.06	0.718

MUAC = mid-upper arm circumference; bz = standardised regression coefficient; Mar = marijuana; Met = methamphetamine.

Significant at p<0.05.

Table 6 summarises the multiple regression results for 1YL. A positive association between infant length at 1 year and MUAC was only found for the Control and NoDrugsSmoke groups. The Control group had the largest MUACs, which was associated with the tallest infants at 1 year, whereas the NoDrugsSmoke group had significantly smaller MUACs and significantly shorter infants at 1 year when compared with the Control group. Infant length at 1 year was also positively associated with education of mothers in the Control, NoDrugsDrink, NoDrugsSmoke, NoDrugsDrinkSmoke and MetDrinkSmoke groups. Education was highest in the Control and NoDrugsDrink groups, with the tallest infants at 1 year, and lowest in smoking plus drug use groups, and these infants were also significantly shorter at 1 year, as seen in the MetDrinkSmoke group. There was a positive association between 1YL of the infant and income of the mother for the NoDrugsSmoke and NoDrugsDrinkSmoke groups. Those who had a higher income in these groups had taller infants at 1 year.

Table 6.

Multiple regression summary for outcome variable infant length at 1 year

		MUAC		Education		Income
Substance use group	n	bz	p-value	bz	p-value	bz	p-value
Control	592	0.13	0.001*	0.10	0.014*	0.02	0.557
NoDrugsDrink	545	0.06	0.144	0.18	<0.001*	0.07	0.095
NoDrugsSmoke	522	0.09	0.045*	0.10	0.035*	0.12	0.011*
NoDrugsDrinkSmoke	1 089	0.05	0.104	0.14	<0.001*	0.14	<0.001*
MarSmoke	35	−0.05	0.765	0.33	0.073	0.24	0.193
MetSmoke	14	0.44	0.313	−0.44	0.223	0.16	0.721
MarDrink	10	−0.24	0.582	0.78	0.181	−0.51	0.424
MetDrink	6	0.37	0.742	0.34	0.670	0.08	0.943
MarDrinkSmoke	143	0.08	0.362	0.16	0.058	0.16	0.075
MetDrinkSmoke	47	0.21	0.096	0.45	0.001*	0.18	0.159
All	42	0.19	0.233	0.29	0.113	−0.05	0.786

MUAC = mid-upper arm circumference; bz = standardised regression coefficient; Mar = marijuana; Met = methamphetamine.

Significant at p<0.05.

Table 7 summarises the multiple regression results for 1YHC. Infant head circumference at 1 year was not associated with MUAC, but was positively associated with maternal education for the Control, NoDrugsDrink, NoDrugsDrinkSmoke, MarDrinkSmoke and MetDrinkSmoke groups. Higher education was associated with larger head circumferences and lower education was associated with smaller head circumferences in these groups. In the NoDrugsDrinkSmoke group, 1YHC was positively associated with income. Those who had a higher income in this group also had infants with larger 1YHC.

Table 7.

Multiple regression summary for outcome infant head circumference at 1 year

		MUAC		Education		Income
Substance use group	n	bz	p-value	bz	p-value	bz	p-value
Control	604	0.07	0.077	0.10	0.013*	0.04	0.378
NoDrugsDrink	549	0.01	0.730	0.13	0.003*	−0.01	0.823
NoDrugsSmoke	536	0.00	0.930	0.08	0.090	0.09	0.060
NoDrugsDrinkSmoke	1 105	0.03	0.391	0.11	0.001*	0.08	0.015*
MarSmoke	37	0.12	0.519	0.15	0.438	−0.11	0.576
MetSmoke	15	0.45	0.304	−0.30	0.391	−0.25	0.580
MarDrink	10	−0.01	0.977	0.80	0.181	−0.44	0.498
MetDrink	6	0.75	0.278	0.50	0.303	0.93	0.223
MarDrinkSmoke	145	−0.02	0.775	0.23	0.007*	0.07	0.404
MetDrinkSmoke	47	0.03	0.823	0.66	<0.001*	0.14	0.201
All	43	0.28	0.089	−0.05	0.801	−0.11	0.563

MUAC = mid-upper arm circumference; bz = standardised regression coefficient; Mar = marijuana; Met = methamphetamine.

Significant at p<0.05.

Discussion

Maternal measures and trends

We found a significant trend in the GA at enrolment, when women booked for antenatal care, from the earliest GA in women who took no substances to a later GA in those who used all substances, but the MetSmoke and MetDrink groups enrolled even later (Fig. 1). The finding of McCalla et al.[ that, although recreational drug users had a wide range of social problems that compromised fetal growth and development and were in greater need of prenatal care, they were less likely to make use of antenatal care services, supports our finding.

There was also a trend in maternal age (Fig. 2), with the oldest women in the Control group to the youngest in the All group, except for the MarSmoke, MarDrink and MarDrinkSmoke groups. Women who used marijuana were the youngest. Our finding that marijuana users are young is in agreement with other researchers.[

The trend in MUAC (Fig. 3), from no substance users to users of all substances, was significantly smaller MUACs, but MUACs were even smaller in the MarSmoke, MetSmoke and MarDrinkSmoke groups. Our finding that women who smoked, whether combined with drugs and/or alcohol or not, had significantly smaller MUACs, has been confirmed by two previous studies.[ The reduced MUAC, associated with cigarette smoking and indicating poorer nutritional status, was associated with an increased risk of spontaneous preterm birth as well as a lower infant BWZ.[

The trend in education (Fig. 4) and income (Table 2) from Control to All was lower education and lower income with more substances used. Women who smoked, in any combination, all had significantly lower education when compared with the Control group or drinkers only. Numerous studies that have reported on the association of cigarette smoking with a lower level of education[ and income[ support our finding. Compared with the women in the Control group, women in the NoDrugsDrink group had a higher education, and women who drank combined with marijuana or methamphetamine, but did not smoke, did not differ significantly. Women in the NoDrugsDrink, MarDrink and Control groups had the highest mean education, ranging from 10.5 to 10.8 years. This finding is validated by research by Patrick et al.,[ who reported that young adults with the highest family education and income were most prone to alcohol and marijuana use, and by Rees,[ who found little evidence that drinking affected educational attainment.

Birth outcomes and trends

Gestation at delivery declined as the number of substances increased, although this did not apply to alcohol use alone. Compared with the Control group, GA at delivery was significantly lower for methamphetamine users and for smoking on its own or in combination with marijuana, while it was significantly higher for the NoDrugsDrink group (Fig. 5), with the highest mean GA of 39 weeks and 2 days. There was no significant difference between the Control and NoDrugsDrinkSmoke, MarDrink or MarDrinkSmoke groups. Our previous study also found that alcohol use alone was associated with a higher GA, while alcohol seemed to counteract the negative association of smoking with GA,[ and lends support to our findings. The highest significant difference in GA was found when we compared the NoDrugsDrink group (highest GA) with the MetDrink group (lowest GA). This suggests a combined effect of methamphetamine and alcohol on GA. Not only did the MetDrink group have the most preterm births, but it also had the highest significant rate of stillbirths, despite being such a small group. Our results endorse the findings of other researchers that methamphetamine was associated with a lower GA at birth[ and with preterm birth.[ However, according to England et al.,[ little is known about the co-use of other substances by women who drink during pregnancy. It appears that the combined effect of methamphetamine and alcohol on GA has not been reported previously. It is interesting that Sowell et al. [ found that brain morphology was affected in children with prenatal methamphetamine and alcohol exposure above and beyond the effects of alcohol exposure alone, suggesting a synergistic effect between methamphetamine and alcohol.

The trend in birthweight from Control to All was lower birthweight with more substances used (Fig. 6). Okah et al.[ reported that women with alcohol and/or drug use during pregnancy did not appear to be at greater risk of giving birth to a term LBW infant than women who reported abstinence. However, the addition of smoking to either behaviour produces placental vasoconstriction that will decrease oxygen delivery to the fetus, limit fetal growth,[ and increase the risk of LBW by 2- to 4-fold. Gibson et al.[ found that infants born to smokers had lower birthweights and were more prone to GR. These reports support our findings of significantly more infants with LBW in the smoking groups (NoDrugsSmoke, NoDrugsDrinkSmoke, MarDrinkSmoke and All) and of the non-smoking groups (all but one) being the only groups with a mean birthweight >3 000 g (Table 3). The MetDrink group, being the exception, had the lowest mean birthweight and also the lowest mean GA at delivery (<37 weeks), with 45.5% of infants being preterm. Many researchers have found that methamphetamine was associated with lower birthweight,[ and Black et al. [ found antenatal drug use to increase the risk of LBW infants above that related to cigarette smoking. Odendaal et al.[ and Jackson et al.[ reported that the combined use of cigarettes and alcohol during pregnancy had a synergistic effect for LBW and GR, which also concurs with our findings.

The trend in BWZ from Control to All was lower BWZs with more substances used. The lowest BWZs were associated with marijuana and smoking, but not methamphetamine (Fig. 7). Significant GR was detected in the infants from the smoking groups (NoDrugsSmoke, NoDrugsDrinkSmoke, MarDrinkSmoke, and All), with >25% of the MarDrinkSmoke group being affected. El Marroun et al. [ reported that marijuana use during pregnancy resulted in more pronounced GR than tobacco use, while Sturrock et al.[ also found that cigarette smoking was associated with a lower BWZ, but that women who both smoked and used marijuana during pregnancy had infants with a lower BWZ than those who used cigarettes alone. Spinillo et al.[ reported on fetal GR among women who smoked throughout pregnancy, while Hayatbakhsh et al.,[ after controlling for smoking, alcohol consumption and other drugs, showed that marijuana use in pregnancy was associated with SGA infants with lower BWZs. The abovementioned researchers all validate our findings.

One-year outcomes and trends

The trend in infant weight from Control to All was lower infant weight at 1 year with more substances used (Fig. 8). The lowest weights were in the methamphetamine-using groups, especially the MetDrink group, which had the lowest mean weight, with the most preterm births and infant ages adjusted for prematurity, and the MetSmoke group. In previous studies, weight and growth were reported as significantly decreased in methamphetamine-exposed children at ages 1 – 4 years,[ which endorses our results.

The trend in infant length from Control to All was shorter infant length at 1 year with more substances used (Fig. 9). Smoking only, or smoking combined with drugs and/or alcohol, was associated with significantly shorter infants at 1 year. Many studies have shown a long-term negative effect of maternal smoking during pregnancy on height of infants, from birth to adolescence,[ which supports our finding. Zabaneh et al.,[ Smith et al.[ and Eriksson et al.[ reported decreased height velocity throughout the first 3 years of life in methamphetamine-exposed children, corroborating our findings that infants from the MetDrink and MetSmoke groups, although adjusted for prematurity, had the shortest and second-shortest mean length at 1 year, respectively (Table 3).

The trend in infant head circumference from Control to All was a smaller 1YHC with more substances used. The smallest head circumferences were in the MetDrink group, despite adjustment for prematurity (Fig. 10). Other researchers have found that infants prenatally exposed to methamphetamine tended to show a significantly smaller head circumference at birth or 1 year,[ supporting our findings.

Effects of combined drug use, smoking and drinking on maternal measures, birth and 1-year outcome

Many significant differences were found when the MarDrinkSmoke and MetDrinkSmoke groups, who used three substances, were compared with the Control group. Women using three substances (methamphetamine or marijuana with smoking and drinking) were younger, had a smaller MUAC, lower education and smaller income, and had infants with lower birthweight, 1-year weight and 1-year height than those from the Control group. These results are supported by the findings of other researchers.[ Although polysubstance use in pregnancy is common,[ there is little information available, and the full range of substance combinations and their health impacts remain incompletely understood.[ Alcohol, tobacco and drug co-use during pregnancy is particularly problematic and compounds the adverse effects on fetal growth.[

Women in the methamphetamine three-substance (MetDrinkSmoke) group enrolled much later and had a lower GA at birth than Controls. They were also older than marijuana users but younger than abstainers. Smith et al.[ found that infants exposed to methamphetamine or tobacco during pregnancy were 3.5 times or 2 times more likely, respectively, to be SGA compared with unexposed infants, suggesting more GR if the infant was exposed to methamphetamine and smoking. GR together with our finding of lower GA in the MetDrinkSmoke group (17% preterm births, which was second highest after the 45.5% in the MetDrink group) supports the association of methamphetamine with preterm birth.

Women in the marijuana three-substance (MarDrinkSmoke) group were much younger (also younger than methamphetamine users), had lower gravidity, were significantly more anaemic, had infants with a lower BWZ and smaller head circumference, and had more LBW and SGA infants when compared with the Control group. Interestingly, Chabarria et al.[ and Grzeskowiak et al.[ reported decreased head circumference at birth to be associated with maternal marijuana use combined with smoking, or independent of tobacco use, respectively. This may help explain the association found between MarDrinkSmoke and smaller head circumference of infants at 1 year in our study. Although we agree with others that marijuana use in pregnancy is harmful to the fetus in that it was associated with low infant birthweight[ and SGA infants,[ our findings support those of Conner et al.[ and Forray et al.,[ who reported that the association between maternal marijuana use and adverse outcomes appears to be attributable to comorbid substance use. Our findings are consistent with many reports of marijuana users being younger,[ of lower parity, better educated, and more likely to use alcohol, cigarettes and hard drugs.[ However, we found no direct association between marijuana use and spontaneous preterm birth, as others have reported.[

Confounders

Our finding that a larger MUAC, indicative of better nutritional status, was associated with a higher BWZ was supported by Smith et al.,[ who found that lower maternal weight gain during pregnancy was more likely to result in an SGA infant. A larger MUAC was also associated with a taller, heavier infant at 1 year.

Higher education was positively associated with outcomes at birth (BWZ) and all outcomes at 1 year, resulting in a larger infant who weighed more, was taller and had a larger head circumference. Numerous researchers have reported a strong inverse relationship between education and cigarette smoking[ and drug use.[ By decreasing substance use, academic outcomes may improve, and therefore also birth and 1-year outcomes.

Higher income was associated with a lower BWZ, perhaps suggesting more methamphetamine and alcohol use while pregnant, but was also associated with a larger infant at 1 year who weighed more, was taller and had a larger head circumference.

Study strengths and limitations

The SPS was a unique, large study performed in population groups with similar socioeconomic circumstances and known to have a high incidence of antenatal substance use. A wealth of maternal, fetal and infant data were collected prospectively over a 9-year period. Substance use exposure data were collected on up to four occasions throughout pregnancy, and infant assessments were done at up to three time points throughout the first year of life. All measurements were taken twice, and we used validated recognised instruments and adjusted 1-year infant age for prematurity.

Limitations include that despite this being a large study with a high incidence of substance use, the small numbers in certain substance use groups limit the strength of the findings. Substance use was self-reported and may therefore be under-reported. Although we have detailed smoking and drinking exposure continuous data, drug information was not quantified, limiting us to nominal (yes or no) data for the various substances used.

Conclusion

The tragedy of substance use during pregnancy not only affects maternal and fetal health during pregnancy, but also infant growth and wellbeing at 1 year of age. Given that these substances are modifiable risk factors,[ and that detailed information on the preventable adverse effects of smoking and drinking during pregnancy was not effective in the population studied,[ it is clearly a major public health problem. The co-use of methamphetamine and alcohol (smallest group) seemed to have a confounding negative association with infant birth and 1-year outcomes, but reasons for this remain unknown. The addictive properties of substance use make cessation difficult, so prevention strategies should rather be addressed. As the prevalence of tobacco use among 13 – 15-year-old females in SA was 20% in 2002,[ prevention strategies should be implemented long before pregnancy in order to limit the uptake of addictive substance use among young women. Higher maternal education, associated with better infant outcomes at birth and 1 year and acting as a countermeasure to substance use, is of paramount importance.