Literature DB >> 36205898

Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Released.

Soodabeh Majdi1, Alex S Lima1, Andrew G Ewing2.   

Abstract

We review the methods of vesicle impact electrochemical cytometry, intracellular impact electrochemical cytometry, and single cell amperometry and their application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluate factors related to neuronal function at the cellular level and implications in, for example, cognition.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Electrochemical cytometry; Electrochemistry; Exocytosis; Neurotransmitters; Vesicles

Mesh：

Substances：

Year:  2023        PMID： 36205898     DOI： 10.1007/978-1-0716-2671-9_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  On the mechanism of electrochemical vesicle cytometry: chromaffin cell vesicles and liposomes.

Authors:  Jelena Lovrić; Neda Najafinobar; Johan Dunevall; Soodabeh Majdi; Irina Svir; Alexander Oleinick; Christian Amatore; Andrew G Ewing
Journal:  Faraday Discuss       Date:  2016-12-12       Impact factor: 4.008

2.  Open carbon nanopipettes as resistive-pulse sensors, rectification sensors, and electrochemical nanoprobes.

Authors:  Keke Hu; Yixian Wang; Huijing Cai; Michael V Mirkin; Yang Gao; Gary Friedman; Yury Gogotsi
Journal:  Anal Chem       Date:  2014-09-02       Impact factor: 6.986

Review 3.  Chemical Analysis of Single Cells and Organelles.

Authors:  Keke Hu; Tho D K Nguyen; Stefania Rabasco; Pieter E Oomen; Andrew G Ewing
Journal:  Anal Chem       Date:  2020-12-07       Impact factor: 6.986

4.  Enterochromaffin Cells Are Gut Chemosensors that Couple to Sensory Neural Pathways.

Authors:  Nicholas W Bellono; James R Bayrer; Duncan B Leitch; Joel Castro; Chuchu Zhang; Tracey A O'Donnell; Stuart M Brierley; Holly A Ingraham; David Julius
Journal:  Cell       Date:  2017-06-22       Impact factor: 41.582

5.  Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells.

Authors:  R H Chow; L von Rüden; E Neher
Journal:  Nature       Date:  1992-03-05       Impact factor: 49.962

Review 6.  Neuropods.

Authors:  Rodger A Liddle
Journal:  Cell Mol Gastroenterol Hepatol       Date:  2019-01-30

7.  Dynamic Visualization and Quantification of Single Vesicle Opening and Content by Coupling Vesicle Impact Electrochemical Cytometry with Confocal Microscopy.

Authors:  Ying-Ning Zheng; Tho D K Nguyen; Johan Dunevall; Nhu T N Phan; Andrew G Ewing
Journal:  ACS Meas Sci Au       Date:  2021-08-09

8.  Simultaneous detection of vesicular content and exocytotic release with two electrodes in and at a single cell.

Authors:  Chaoyi Gu; Andrew G Ewing
Journal:  Chem Sci       Date:  2021-04-20       Impact factor: 9.825
  8 in total

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