Literature DB >> 3620505

Isolation of dermatan sulfate with high heparin cofactor II-mediated thrombin-inhibitory activity from porcine spleen.

H Munakata, C C Hsu, C Kodama, J Aikawa, M Sakurada, N Ototani, M Isemura, Z Yosizawa, N Hayashi.   

Abstract

We prepared dermatan sulfate specimens from various porcine tissues, and compared their heparin cofactor II-mediated thrombin-inhibitory activities and chemical natures, including disaccharide composition. Electrophoresis of the specimens on cellulose acetate membrane indicated that spleen dermatan sulfate was the most acidic of the dermatan sulfates prepared from the various porcine tissues. Analysis of the disaccharide units of the dermatan sulfate specimens by high-performance liquid chromatography revealed that spleen dermatan sulfate was rich in 4,6-di-O-sulfated N-acetylgalactosamine residues as compared with those of the other tissues. Spleen dermatan sulfate exhibited the highest thrombin-inhibitory activity, which may be related to its high content of the disulfated N-acetylgalactosamine residue.

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Year:  1987        PMID： 3620505     DOI： 10.1016/0304-4165(87)90198-x

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  5 in total

1.  Structural determination of novel tetra- and hexasaccharide sequences isolated from chondroitin sulfate H (oversulfated dermatan sulfate) of hagfish notochord.

Authors:  C Ueoka; S Nadanaka; N Seno; K H Khoo; K Sugahara
Journal:  Glycoconj J       Date:  1999-06       Impact factor: 2.916

Review 2.  Glycosaminoglycans and the regulation of blood coagulation.

Authors:  M C Bourin; U Lindahl
Journal:  Biochem J       Date:  1993-01-15       Impact factor: 3.857

3.  Effect of bitter gourd and spent turmeric on constituents of glycosaminoglycans in different tissues in streptozotocin induced diabetic rats.

Authors:  G Suresh Kumar; B Vijayalakshmi; P V Salimath
Journal:  Mol Cell Biochem       Date:  2006-03-11       Impact factor: 3.396

4.  1H-n.m.r. investigation of naturally occurring and chemically oversulphated dermatan sulphates. Identification of minor monosaccharide residues.

Authors:  V Bossennec; M Petitou; B Perly
Journal:  Biochem J       Date:  1990-05-01       Impact factor: 3.857

5.  Structure and contribution to the heparin cofactor II-mediated inhibition of thrombin of naturally oversulphated sequences of dermatan sulphate.

Authors:  G Mascellani; L Liverani; P Bianchini; B Parma; G Torri; A Bisio; M Guerrini; B Casu
Journal:  Biochem J       Date:  1993-12-15       Impact factor: 3.857
  5 in total

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