| Literature DB >> 36203143 |
Yeda Chen1, Di Gu1, Yaoan Wen1, Shuxin Yang1, Xiaolu Duan1, Yongchang Lai1, Jianan Yang2, Daozhang Yuan2, Aisha Khan3, Wenqi Wu1, Guohua Zeng4.
Abstract
Entities:
Year: 2022 PMID: 36203143 PMCID: PMC9541067 DOI: 10.1186/s12935-022-02707-7
Source DB: PubMed Journal: Cancer Cell Int ISSN: 1475-2867 Impact factor: 6.429
Fig. 4RT-qPCR and western blotting validation of the expression level of SUCLG1, PCK2, GLDC in OSRC-2 and A498 cell lines. a–c Forty-eight hours after plasmid or vector transfection, qPCR detected the expression level of 3 genes in both OSRC-2 and A498 cell lines. b–f Protein expression was evaluated by Western blot. j–l The values of the band intensity represent the densitometric estimation of each band normalised by β-actin in (b–f, respectively). (*p < 0.01)
Fig. 6Over-expression of SUCLG1, PCK2, GLDC inhibits renal carcinoma cell migration and invasion in vitro. a Transwell cell migration assay was performed after the overexpression in OSRC-2 and A498 cells. b Quantitative analysis to (a). c Matrigel cell invasion assay was performed after the overexpression in OSRC-2 and A498 cells. d Quantitative analysis to (c). e–g Overexpression SUCLG1, PCK2, GLDC suppressed wound healing of OSRC-2 and A498 cell line. f–h Quantitative description to (e) and (g). i Cell cycle of overexpression SUCLG1, PCK2, GLDC after transfection 48 h was analyzed by fow cytometry. Image shows a representative experiment out of three. Data was performed as mean ± SD of three independent experiments. (*p < 0.001)