Simin Liu1,2, Jiali Mi1,2, Kejing Song3, Haishan Qi4,5, Lei Zhang1,2. 1. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, No.135, Yaguan Road, Tianjin, 300072, People's Republic of China. 2. School of Chemical Engineering and Technology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), Tianjin University, No.135, Yaguan Road, Tianjin, 300350, People's Republic of China. 3. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China. 4. Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, No.135, Yaguan Road, Tianjin, 300072, People's Republic of China. hsqi@tju.edu.cn. 5. School of Chemical Engineering and Technology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), Tianjin University, No.135, Yaguan Road, Tianjin, 300350, People's Republic of China. hsqi@tju.edu.cn.
Abstract
OBJECTIVES: 1,5-pentanediamine (cadaverine) is a C5 platform chemical, also an important raw material for bio-polyamide PA5X. With increasing concerns about the depletion of fossil resources and global environmental protection, cadaverine bio-production has attracted more attentions. RESULTS: Here, a microbial consortium consisting of Corynebacterium glutamicum cgl-FDK and Escherichia coli BL-ABST-Spy was constructed to de novo synthesize cadaverine utilizing glycerol as the sole carbon resource. The glycerol utilization pathway was initially constructed in C. glutamicum cgl-FDK to produce lysine from glycerol. Then, the pyridoxal 5'-phosphate (PLP) biosynthesis pathway and SpyTag/SpyCatcher protein-ligation system for lysine decarboxylase (CadA) and cadaverine-lysine antiporter protein (CadB) were introduced into E. coli BL-ABST-Spy to synthesize cadaverine from lysine. Furthermore, the fermentation conditions of microbial consortium were optimized and the cadaverine production reached 9.3 g/L with glycerol as the sole carbon source. CONCLUSIONS: This work provides a promising strategy for efficiently producing cadaverine from glycerol with an artificial microbial consortium.
OBJECTIVES: 1,5-pentanediamine (cadaverine) is a C5 platform chemical, also an important raw material for bio-polyamide PA5X. With increasing concerns about the depletion of fossil resources and global environmental protection, cadaverine bio-production has attracted more attentions. RESULTS: Here, a microbial consortium consisting of Corynebacterium glutamicum cgl-FDK and Escherichia coli BL-ABST-Spy was constructed to de novo synthesize cadaverine utilizing glycerol as the sole carbon resource. The glycerol utilization pathway was initially constructed in C. glutamicum cgl-FDK to produce lysine from glycerol. Then, the pyridoxal 5'-phosphate (PLP) biosynthesis pathway and SpyTag/SpyCatcher protein-ligation system for lysine decarboxylase (CadA) and cadaverine-lysine antiporter protein (CadB) were introduced into E. coli BL-ABST-Spy to synthesize cadaverine from lysine. Furthermore, the fermentation conditions of microbial consortium were optimized and the cadaverine production reached 9.3 g/L with glycerol as the sole carbon source. CONCLUSIONS: This work provides a promising strategy for efficiently producing cadaverine from glycerol with an artificial microbial consortium.
Authors: Marta Perez; Marina Calles-Enríquez; Beatriz Del Rio; Begoña Redruello; Anne de Jong; Oscar P Kuipers; Jan Kok; M Cruz Martin; Victor Ladero; Maria Fernandez; Miguel A Alvarez Journal: Sci Rep Date: 2019-11-14 Impact factor: 4.379
Authors: Walter D Carciochi; Luiz H Moro Rosso; Mario A Secchi; Adalgisa R Torres; Seth Naeve; Shaun N Casteel; Péter Kovács; Dan Davidson; Larry C Purcell; Sotirios Archontoulis; Ignacio A Ciampitti Journal: Sci Rep Date: 2019-12-27 Impact factor: 4.379