Shuang Wu1, Yan Tang1, Shaojun Su2, Wenbing Ding3,4, Hualiang He1, Jin Xue1, Qiao Gao1, Lin Qiu1, Youzhi Li5,6,7. 1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China. 2. Agricultural Bureau of Dingcheng County, Changde, 415006, China. 3. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. 4. Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing, Changsha, 410128, China. 5. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China. liyouzhi@hunau.edu.cn. 6. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. liyouzhi@hunau.edu.cn. 7. Hunan Agricultural University, Changsha, 410128, China. liyouzhi@hunau.edu.cn.
Abstract
BACKGROUND: The nutritional signaling pathway regulates an insect's size, development, and lifespan, as well as playing a vital role in reproduction. The insulin/insulin-like growth factor signaling (IIS) pathway plays a key role in the nutrition signaling pathway. As an integral component of the IIS pathway, insulin receptor (InR), a receptor tyrosine kinase, plays a role in the insulin pathway by controlling reproduction in many insect species. However, the precise molecular function of InR in non-model insect reproduction is poorly understood. METHODS: In our study, Chilo suppressalis, a well-known rice pest, was used as a molecular system to determine the role of InR in insect reproduction. Sequencing the InR gene of C. suppressalis, comparing the amino acid sequence-specific structure, and constructing a phylogenetic tree revealed that this gene has four main domains: ligand binding L domain, Furin-like region, fibronectin type III domains, and Tyrosine kinase catalytic domain, which were all highly conserved in insects. RESULTS: By characterizing the spatiotemporal expression profile of InR in different developmental stages and tissues, we found that InR gene expression was highest on the 3-day old in female pupae, 6th instar larvae, and fat body on the 6-day old in female pupae. InR gene expression may promote the molting and pupation of larvae and play a role in reproduction in the fat body. Furthermore, the RNA interference knockdown of InR dramatically reduced yolk deposition and blocked oocyte maturation. After suppression of InR, the expression of several other genes fluctuated to varying degrees. CONCLUSION: In conclusion, InR is vital to reproduction and is expected to become a new target for pest management.
BACKGROUND: The nutritional signaling pathway regulates an insect's size, development, and lifespan, as well as playing a vital role in reproduction. The insulin/insulin-like growth factor signaling (IIS) pathway plays a key role in the nutrition signaling pathway. As an integral component of the IIS pathway, insulin receptor (InR), a receptor tyrosine kinase, plays a role in the insulin pathway by controlling reproduction in many insect species. However, the precise molecular function of InR in non-model insect reproduction is poorly understood. METHODS: In our study, Chilo suppressalis, a well-known rice pest, was used as a molecular system to determine the role of InR in insect reproduction. Sequencing the InR gene of C. suppressalis, comparing the amino acid sequence-specific structure, and constructing a phylogenetic tree revealed that this gene has four main domains: ligand binding L domain, Furin-like region, fibronectin type III domains, and Tyrosine kinase catalytic domain, which were all highly conserved in insects. RESULTS: By characterizing the spatiotemporal expression profile of InR in different developmental stages and tissues, we found that InR gene expression was highest on the 3-day old in female pupae, 6th instar larvae, and fat body on the 6-day old in female pupae. InR gene expression may promote the molting and pupation of larvae and play a role in reproduction in the fat body. Furthermore, the RNA interference knockdown of InR dramatically reduced yolk deposition and blocked oocyte maturation. After suppression of InR, the expression of several other genes fluctuated to varying degrees. CONCLUSION: In conclusion, InR is vital to reproduction and is expected to become a new target for pest management.
Authors: Sandrien Van de Velde; Liesbeth Badisco; Ilse Claeys; Peter Verleyen; Xi Chen; Luc Vanden Bosch; Jozef Vanden Broeck; Guy Smagghe Journal: Gen Comp Endocrinol Date: 2007-05-08 Impact factor: 2.822
Authors: David S Richard; Robert Rybczynski; Thomas G Wilson; Yue Wang; Marta L Wayne; You Zhou; Linda Partridge; Lawrence G Harshman Journal: J Insect Physiol Date: 2005-04 Impact factor: 2.354
Authors: Susan J Broughton; Matthew D W Piper; Tomoatsu Ikeya; Timothy M Bass; Jake Jacobson; Yasmine Driege; Pedro Martinez; Ernst Hafen; Dominic J Withers; Sally J Leevers; Linda Partridge Journal: Proc Natl Acad Sci U S A Date: 2005-02-11 Impact factor: 11.205