| Literature DB >> 3619032 |
Y Shimomura, R Paxton, T Ozawa, R A Harris.
Abstract
A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain alpha-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of E1 alpha, E1 beta, and E2 subunits, appeared homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contained endogenous kinase activity for phosphorylation and inactivation of the complex.Entities:
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Year: 1987 PMID： 3619032 DOI： 10.1016/0003-2697(87)90094-7
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365