Literature DB >> 36180702

Measurement of NQO2 Catalytic Activity and of Its Inhibition by Melatonin.

Gilles Ferry1, Jean A Boutin2,3.   

Abstract

The third melatonin binding site MT3 turned out to be an enzyme, NQO2 (E.C. 1.6.99.2). Its catalytic activity is inhibited by melatonin with an IC50 in the 50-100 μM range. Some of the functions of melatonin at pharmacological concentrations (1 μM and above) might be explained by this inhibition capacity of melatonin at NQO2. In order to determine precisely these parameters, it is required to comprehend the basic enzymology of this enzyme. In the following chapter, we present the basic conditions of measuring NQO2 catalytic activities and inhibition.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Catalytic activity; MT3; Melatonin inhibition; NQO2

Mesh：

Substances：

Year:  2022        PMID： 36180702     DOI： 10.1007/978-1-0716-2593-4_33

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  22 in total

1.  Redox properties of the [2Fe-2S] center in the 24 kDa (NQO2) subunit of NADH:ubiquinone oxidoreductase (complex I).

Authors:  Yanbing Zu; Salvatore Di Bernardo; Takao Yagi; Judy Hirst
Journal:  Biochemistry       Date:  2002-08-06       Impact factor: 3.162

2.  Mitochondrial oxidation of extra-mitochondrial TPNH1 mediated by purified DT diaphorase.

Authors:  T E CONOVER; L ERNSTER
Journal:  Biochem Biophys Res Commun       Date:  1960-01       Impact factor: 3.575

3.  Journal of Pineal Research guideline for authors: Defining and characterizing melatonin targets.

Authors:  Erika Cecon; Céline Legros; Jean A Boutin; Ralf Jockers
Journal:  J Pineal Res       Date:  2020-12-17       Impact factor: 13.007

Review 4.  NRH:quinone reductase 2: an enzyme of surprises and mysteries.

Authors:  Fanny Vella; Gilles Ferry; Philippe Delagrange; Jean A Boutin
Journal:  Biochem Pharmacol       Date:  2005-10-25       Impact factor: 5.858

5.  Characterization of 2-[125I]iodomelatonin binding sites in hamster brain.

Authors:  M J Duncan; J S Takahashi; M L Dubocovich
Journal:  Eur J Pharmacol       Date:  1986-12-16       Impact factor: 4.432

6.  Identification of the melatonin-binding site MT3 as the quinone reductase 2.

Authors:  O Nosjean; M Ferro; F Coge; P Beauverger; J M Henlin; F Lefoulon; J L Fauchere; P Delagrange; E Canet; J A Boutin
Journal:  J Biol Chem       Date:  2000-10-06       Impact factor: 5.157

7.  2-[125I]iodomelatonin binding sites in hamster brain membranes: pharmacological characteristics and regional distribution.

Authors:  M J Duncan; J S Takahashi; M L Dubocovich
Journal:  Endocrinology       Date:  1988-05       Impact factor: 4.736

Review 8.  Is There Sufficient Evidence that the Melatonin Binding Site MT3 Is Quinone Reductase 2?

Authors:  Jean A Boutin; Gilles Ferry
Journal:  J Pharmacol Exp Ther       Date:  2018-11-02       Impact factor: 4.030

9.  Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress.

Authors:  Manoj Sonavane; Faisal Hayat; Mikhail Makarov; Marie E Migaud; Natalie R Gassman
Journal:  PLoS One       Date:  2020-11-09       Impact factor: 3.240
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