Detection of Melatonin in Tissue Samples.

Melatonin is present in higher quantity in brain during the night. The variation of its quantity is not only a matter of day/night cycle but also a matter of organ and tissues' sublocalization. It is of the highest importance to be able to precisely measure these quantities, and thus, these variations, particularly to better understand the way melatonin signals its presence and the variation thereof through many putative targets. In this chapter, we detail the way these measures can be performed.