Literature DB >> 36180623

Adoptive Transfer and Bone Marrow Chimera Models to Analyze Treg Function and Differentiation.

Vital da Silva Domingues1,2, Iris Caramalho1, Marie-Louise Bergman1, Jocelyne Demengeot3.   

Abstract

Cellular adoptive transfer and mixed bone marrow chimera are cornerstone experimental tools for immuno-biology. Here we describe protocols for adoptive transfer and bone marrow chimera to address the effect of a specific mutation on T regulatory cell (Treg) function and differentiation, respectively. Treg function can be quantitatively measured by analyzing the expansion of conventional CD4 T cells and their differentiation into helper cells. The quantitative measure of Treg differentiation is addressed by analyzing the number and phenotype of Foxp3-expressing cells. The use of congenic markers is instrumental for these approaches.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Adoptive transfer; Bone Marrow; Chimera; Differentiation; Disease; Function; Homeostasis; Mice; Proliferation; Regulatory T cells

Mesh：

Substances：

Year:  2023        PMID： 36180623     DOI： 10.1007/978-1-0716-2647-4_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  CD25+ CD4+ T cells regulate the expansion of peripheral CD4 T cells through the production of IL-10.

Authors:  O Annacker; R Pimenta-Araujo; O Burlen-Defranoux; T C Barbosa; A Cumano; A Bandeira
Journal:  J Immunol       Date:  2001-03-01       Impact factor: 5.422

2.  Control of regulatory T cell development by the transcription factor Foxp3.

Authors:  Shohei Hori; Takashi Nomura; Shimon Sakaguchi
Journal:  Science       Date:  2003-01-09       Impact factor: 47.728

3.  Extrathymically generated regulatory T cells control mucosal TH2 inflammation.

Authors:  Steven Z Josefowicz; Rachel E Niec; Hye Young Kim; Piper Treuting; Takatoshi Chinen; Ye Zheng; Dale T Umetsu; Alexander Y Rudensky
Journal:  Nature       Date:  2012-02-08       Impact factor: 49.962

4.  Regulatory T cells contribute to diabetes protection in lipopolysaccharide-treated non-obese diabetic mice.

Authors:  I Caramalho; L Rodrigues-Duarte; A Perez; S Zelenay; C Penha-Gonçalves; J Demengeot
Journal:  Scand J Immunol       Date:  2011-12       Impact factor: 3.487

5.  CD25+CD4+ regulatory T cells suppress CD4+ T cell-mediated pulmonary hyperinflammation driven by Pneumocystis carinii in immunodeficient mice.

Authors:  Shohei Hori; Thiago L Carvalho; Jocelyne Demengeot
Journal:  Eur J Immunol       Date:  2002-05       Impact factor: 5.532

6.  Foxp3 programs the development and function of CD4+CD25+ regulatory T cells.

Authors:  Jason D Fontenot; Marc A Gavin; Alexander Y Rudensky
Journal:  Nat Immunol       Date:  2003-03-03       Impact factor: 25.606

7.  Foxp3+ CD25- CD4 T cells constitute a reservoir of committed regulatory cells that regain CD25 expression upon homeostatic expansion.

Authors:  Santiago Zelenay; Thiago Lopes-Carvalho; Iris Caramalho; Maria Francisca Moraes-Fontes; Manuel Rebelo; Jocelyne Demengeot
Journal:  Proc Natl Acad Sci U S A       Date:  2005-03-07       Impact factor: 11.205

8.  Homeostasis of peripheral CD4+ T cells: IL-2R alpha and IL-2 shape a population of regulatory cells that controls CD4+ T cell numbers.

Authors:  Afonso R M Almeida; Nicolas Legrand; Martine Papiernik; António A Freitas
Journal:  J Immunol       Date:  2002-11-01       Impact factor: 5.422

9.  Recent thymic emigrants are the preferential precursors of regulatory T cells differentiated in the periphery.

Authors:  Ricardo S Paiva; Andreia C Lino; Marie-Louise Bergman; Iris Caramalho; Ana E Sousa; Santiago Zelenay; Jocelyne Demengeot
Journal:  Proc Natl Acad Sci U S A       Date:  2013-04-01       Impact factor: 11.205

10.  Organ-specific autoimmune diseases induced in mice by elimination of T cell subset. I. Evidence for the active participation of T cells in natural self-tolerance; deficit of a T cell subset as a possible cause of autoimmune disease.

Authors:  S Sakaguchi; K Fukuma; K Kuribayashi; T Masuda
Journal:  J Exp Med       Date:  1985-01-01       Impact factor: 14.307
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