Glycosylated components induced in African swine fever (ASF) virus-infected Vero cells.

African swine fever (ASF) virus production was inhibited more than 100 fold by 5 mM glucosamine, 2 mM 2-deoxyglucose and 3 microM tunicamycin. ASF virus induced in Vero cells the synthesis of 19 glycosylated components of molecular weights ranging from 9K to 220K, the major ones being those of 9K, 13K, 14K, 74K and 220K. At least five of the induced glycosylated components, of molecular weights 13K, 33K, 34K, 38K and 220K, were probably virus-coded glycoproteins, as suggested by a comparative analysis of the time course of synthesis and the antigenicity of these components in extracts from [35S]methionine or [14C]glucosamine-labeled infected cells. The non-protein glycosylated components present in extracellular ASF virus particles had a cellular origin.