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Literature DB >> 361699

Absence of DNA sequences homologous to transposable element Tn5 (Kan) in the chromosome of Escherichia coli K-12.

Abstract

The DNA hybridization procedure of Southern has been used to search for homology between the transposable kanamycin resistance determinant Tn5 and sequences in the chromosome of Escherichia coli K-12. No homology was detected under conditions in which a segment homologous to 5% or more of the 5,300-base pair Tn5 element would have been seen.

Entities: Chemical Species

Mesh：

Substances：
DNA, Bacterial
Kanamycin

Year: 1978 PMID： 361699 PMCID： PMC218674 DOI： 10.1128/jb.136.1.419-422.1978

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

1. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.

Authors: K Murray; N E Murray
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. The nature and origin of a class of essential gene substitutions in bacteriophage lambda.

Authors: D Henderson; J Weil
Journal: Virology Date: 1975-09 Impact factor: 3.616

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

4. DNA sequence at the integration sites of the insertion element IS1.

Authors: M P Calos; L Johnsrud; J H Miller
Journal: Cell Date: 1978-03 Impact factor: 41.582

5. Characterization of bacteriophage lambda reverse as an Escherichia coli phage carrying a unique set of host-derived recombination functions.

Authors: M M Gottesman; M E Gottesman; S Gottesman; M Gellert
Journal: J Mol Biol Date: 1974-09-15 Impact factor: 5.469

6. IS-elements in microorganisms.

Authors: P Starlinger; H Saedler
Journal: Curr Top Microbiol Immunol Date: 1976 Impact factor: 4.291

7. Bacteriophage Mu-1: a tool to transpose and to localize bacterial genes.

Authors: M Faelen; A Toussaint
Journal: J Mol Biol Date: 1976-07-05 Impact factor: 5.469

8. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages.

Authors: K Ippen; J A Shapiro; J R Beckwith
Journal: J Bacteriol Date: 1971-10 Impact factor: 3.490

9. Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.

Authors: G Ketner; T J Kelly
Journal: Proc Natl Acad Sci U S A Date: 1976-04 Impact factor: 11.205

10. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.

Authors: V Hershfield; H W Boyer; C Yanofsky; M A Lovett; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1974-09 Impact factor: 11.205

14 in total

1. Deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms.

Authors: P Nisen; M Purucker; L Shapiro
Journal: J Bacteriol Date: 1979-11 Impact factor: 3.490

Absence of DNA sequences homologous to transposable element Tn5 (Kan) in the chromosome of Escherichia coli K-12.

1. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.

2. The nature and origin of a class of essential gene substitutions in bacteriophage lambda.

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

4. DNA sequence at the integration sites of the insertion element IS1.

5. Characterization of bacteriophage lambda reverse as an Escherichia coli phage carrying a unique set of host-derived recombination functions.

6. IS-elements in microorganisms.

7. Bacteriophage Mu-1: a tool to transpose and to localize bacterial genes.

8. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages.

9. Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.

10. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.

1. Deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms.

2. Construction of a physical map of a kanamycin (Km) transposon, Tn5, and a comparison to another Km transposon, Tn903.

3. The ends of Tn10 are not IS3.

4. Nucleotide sequence analysis of in vivo recombinants between bacteriophage lambda DNA and pBR322.

5. Structural requirement for IS50-mediated gene transposition.

6. Inverted repeats of Tn5 are transposable elements.

7. Sequences essential for transposition at the termini of IS50.

8. In vivo genesis of a transposon carrying the histidine genes of Escherichia coli K-12.

9. A product of the TN5 transposase gene inhibits transposition.

10. Evolution of transposons: natural selection for Tn5 in Escherichia coli K12.