Literature DB >> 36163548

Engineered CRISPR prime editors with compact, untethered reverse transcriptases.

Julian Grünewald1,2,3,4,5,6,7, Bret R Miller8,9, Regan N Szalay8,9, Peter K Cabeceiras8,9, Christopher J Woodilla8,9, Eliza Jane B Holtz8,9, Karl Petri8,9,10, J Keith Joung11,12,13.   

Abstract

The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing.
© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.

Entities:  

Year:  2022        PMID： 36163548     DOI： 10.1038/s41587-022-01473-1

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN： 1087-0156            Impact factor:   68.164


  37 in total

1.  Substitution of Asp114 or Arg116 in the fingers domain of moloney murine leukemia virus reverse transcriptase affects interactions with the template-primer resulting in decreased processivity.

Authors:  J Gu; R A Villanueva; C S Snyder; M J Roth; M M Georgiadis
Journal:  J Mol Biol       Date:  2001-01-12       Impact factor: 5.469

2.  Enhanced prime editing systems by manipulating cellular determinants of editing outcomes.

Authors:  Peter J Chen; Jeffrey A Hussmann; Jun Yan; Friederike Knipping; Purnima Ravisankar; Pin-Fang Chen; Cidi Chen; James W Nelson; Gregory A Newby; Mustafa Sahin; Mark J Osborn; Jonathan S Weissman; Britt Adamson; David R Liu
Journal:  Cell       Date:  2021-10-14       Impact factor: 66.850

3.  Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition.

Authors:  Benjamin P Kleinstiver; Michelle S Prew; Shengdar Q Tsai; Nhu T Nguyen; Ved V Topkar; Zongli Zheng; J Keith Joung
Journal:  Nat Biotechnol       Date:  2015-11-02       Impact factor: 54.908

4.  A design optimized prime editor with expanded scope and capability in plants.

Authors:  Wen Xu; Yongxing Yang; Biying Yang; Christopher J Krueger; Qianlin Xiao; Si Zhao; Lu Zhang; Guiting Kang; Feipeng Wang; Hongmei Yi; Wen Ren; Lu Li; Xiaoqing He; Chuanmao Zhang; Bo Zhang; Jiuran Zhao; Jinxiao Yang
Journal:  Nat Plants       Date:  2021-12-23       Impact factor: 17.352

5.  GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases.

Authors:  Shengdar Q Tsai; Zongli Zheng; Nhu T Nguyen; Matthew Liebers; Ved V Topkar; Vishal Thapar; Nicolas Wyvekens; Cyd Khayter; A John Iafrate; Long P Le; Martin J Aryee; J Keith Joung
Journal:  Nat Biotechnol       Date:  2014-12-16       Impact factor: 54.908

6.  BE-PIGS: a base-editing tool with deaminases inlaid into Cas9 PI domain significantly expanded the editing scope.

Authors:  Yanhong Wang; Lifang Zhou; Nan Liu; Shaohua Yao
Journal:  Signal Transduct Target Ther       Date:  2019-09-20

7.  Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure.

Authors:  Xiaosa Li; Lina Zhou; Bao-Qing Gao; Guangye Li; Xiao Wang; Ying Wang; Jia Wei; Wenyan Han; Zixian Wang; Jifang Li; Runze Gao; Junjie Zhu; Wenchao Xu; Jing Wu; Bei Yang; Xiaodong Sun; Li Yang; Jia Chen
Journal:  Nat Commun       Date:  2022-03-29       Impact factor: 14.919

8.  High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.

Authors:  Benjamin P Kleinstiver; Vikram Pattanayak; Michelle S Prew; Shengdar Q Tsai; Nhu T Nguyen; Zongli Zheng; J Keith Joung
Journal:  Nature       Date:  2016-01-06       Impact factor: 49.962

9.  Search-and-replace genome editing without double-strand breaks or donor DNA.

Authors:  Andrew V Anzalone; Peyton B Randolph; Jessie R Davis; Alexander A Sousa; Luke W Koblan; Jonathan M Levy; Peter J Chen; Christopher Wilson; Gregory A Newby; Aditya Raguram; David R Liu
Journal:  Nature       Date:  2019-10-21       Impact factor: 69.504

10.  CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells.

Authors:  Karl Petri; Weiting Zhang; Junyan Ma; Andrea Schmidts; Hyunho Lee; Joy E Horng; Daniel Y Kim; Ibrahim C Kurt; Kendell Clement; Jonathan Y Hsu; Luca Pinello; Marcela V Maus; J Keith Joung; Jing-Ruey Joanna Yeh
Journal:  Nat Biotechnol       Date:  2021-04-29       Impact factor: 54.908
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