Haiyang Xia1,2, Hamza Armghan Noushahi2, Aamir Hamid Khan3, Ying Liu2, Andreea Cosoveanu4, Lingli Cui2, Jing Tang2, Shehzad Iqbal5, Shaohua Shu6. 1. College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China. 2. Bio-Pharmaceuticals Institute , Taizhou University, 317000, Taizhou, China. 3. National Key Laboratory of Crop Genetics Improvement, Huazhong Agricultural University, 430070, Wuhan, China. 4. Department of Botany, Ecology & Plant Physiology, CIPEV Group, Faculty of Science, Biology Section, Universidad de La Laguna, 38206, San Cristobal de La Laguna, Tenerife, Spain. 5. Faculty of Agricultural Sciences, University of Talca, 3460000, Talca, Chile. 6. Bio-Pharmaceuticals Institute , Taizhou University, 317000, Taizhou, China. shushaohua@mail.hzau.edu.cn.
Abstract
BACKGROUND: Colletotrichum gloeosporioides ES026, isolated as an endophytic fungal strain, was found to produce the important medicinal compound HuperzineA (HupA). In a genetic context, ES026 showed potential in elucidating the biosynthetic pathway of HupA. METHODS AND RESULTS: The ES026 strain was sequenced using de-novo Illumina sequencing methods in this study. Assembling the cleaned data resulted in 58,594,804bp, consisting of 404 scaffolds. The G + C mol % content of this genome was 52.53%. The genome progressive-alignment with other 4 Colletotrichum strains revealed that ES026 showed closer relation with 030206, SMCG1#C and Nara gc5. More than 60 putative biosynthetic clusters were predicted with the fungal version antiSMASH4.0 program. More than 33 types I polyketide-related biosynthetic gene clusters were distributed, containing PKS and PKS-NRPS (polyketide-nonribosomal peptides) hybrid gene clusters. Another 8 NRPS biosynthetic gene clusters were distributed among the genome of ES026. The prenyltransferases, probably involved in aromatic prenyl-compounds and terpenoid biosynthesis, were analyzed using bioinformatics tools like MEGA. CONCLUSION: We predicted a new possible biosynthetic pathway for the HupA from the pipecolic acid, based on the published HupA biosynthesis proposed pathway, the biosynthesis and pipecolic acid-derived compounds. We hypothesize that a hybrid PKS-NRPS mega-enzyme was probably involved in the biosynthesis of HupA with the pipecolic acid, the building block of rapamycin, as a HupA precursor. The rapamycin is produced from a polyketide biosynthesis pathway, and the domain incorporating the pipecolic acid is studied.
BACKGROUND: Colletotrichum gloeosporioides ES026, isolated as an endophytic fungal strain, was found to produce the important medicinal compound HuperzineA (HupA). In a genetic context, ES026 showed potential in elucidating the biosynthetic pathway of HupA. METHODS AND RESULTS: The ES026 strain was sequenced using de-novo Illumina sequencing methods in this study. Assembling the cleaned data resulted in 58,594,804bp, consisting of 404 scaffolds. The G + C mol % content of this genome was 52.53%. The genome progressive-alignment with other 4 Colletotrichum strains revealed that ES026 showed closer relation with 030206, SMCG1#C and Nara gc5. More than 60 putative biosynthetic clusters were predicted with the fungal version antiSMASH4.0 program. More than 33 types I polyketide-related biosynthetic gene clusters were distributed, containing PKS and PKS-NRPS (polyketide-nonribosomal peptides) hybrid gene clusters. Another 8 NRPS biosynthetic gene clusters were distributed among the genome of ES026. The prenyltransferases, probably involved in aromatic prenyl-compounds and terpenoid biosynthesis, were analyzed using bioinformatics tools like MEGA. CONCLUSION: We predicted a new possible biosynthetic pathway for the HupA from the pipecolic acid, based on the published HupA biosynthesis proposed pathway, the biosynthesis and pipecolic acid-derived compounds. We hypothesize that a hybrid PKS-NRPS mega-enzyme was probably involved in the biosynthesis of HupA with the pipecolic acid, the building block of rapamycin, as a HupA precursor. The rapamycin is produced from a polyketide biosynthesis pathway, and the domain incorporating the pipecolic acid is studied.
Authors: Ya Wang; Qing Gui Zeng; Zhi Bin Zhang; Ri Ming Yan; Ling Yun Wang; Du Zhu Journal: J Ind Microbiol Biotechnol Date: 2010-11-24 Impact factor: 3.346
Authors: Ugur Damar; Roman Gersner; Joshua T Johnstone; Steven Schachter; Alexander Rotenberg Journal: Med Hypotheses Date: 2016-12-22 Impact factor: 1.538